云南漢族多形性日光疹與HLA-DQA1、DQB1等位基因相關(guān)性研究
發(fā)布時間：2018-12-14 18:54
【摘要】：目的探討多形性日光疹(Polymorphic light eruption,PLE)與HLA-DQA1、DQB1等位基因的相關(guān)性,擬明確PLE可能的HLA易感等位基因和(或)保護性基因。 方法采用經(jīng)典的酚-氯仿法從110例PLE患者和170例健康對照的外周靜脈血白細胞中提取基因組DNA,應(yīng)用聚合酶鏈反應(yīng)-直接測序(polymerase chain reaction sequence-based typing,PCR-SBT)的方法對PLE患者和健康對照組進行HLA-DQA1、DQB1等位基因的檢測。 結(jié)果與對照組相比,病例組中HLA-DQA1*0102,DQB1*0302和DQB1*0601(OR=3.000,Pc=0.0045；OR=7.485,Pc10-3；OR=14.153,Pc10-4)等位基因頻率顯著升高,而DQB1*0201頻率顯著降低(OR=0.354,Pc=0.0280)。 結(jié)論HLA-DQA1*0102,DQB1*0302和DQB1*0601可能是云南漢族PLE的易感基因或與實際的易感基因連鎖,而HLA-DQB1*0201可能為云南漢族PLE的保護性基因。
[Abstract]:Objective to investigate the relationship between (Polymorphic light eruption,PLE and HLA-DQA1,DQB1 alleles and to identify the possible HLA susceptibility alleles and / or protective genes in PLE. Methods Genomic DNA, was extracted from peripheral venous leukocytes of 110 patients with PLE and 170 healthy controls by classical phenol-chloroform method. Polymerase chain reaction-direct sequencing (polymerase chain reaction sequence-based typing, was used. PCR-SBT was used to detect HLA-DQA1,DQB1 alleles in PLE patients and healthy controls. Results compared with the control group, HLA-DQA1*0102,DQB1*0302 and DQB1*0601 (OR=3.000,Pc=0.0045;OR=7.485,Pc10-3;) were found in the case group. The allele frequency of OR=14.153,Pc10-4 increased significantly, while the frequency of DQB1*0201 decreased significantly (OR=0.354,Pc=0.0280). Conclusion HLA-DQA1*0102,DQB1*0302 and DQB1*0601 may be susceptible genes of PLE or linked to actual susceptibility genes in Yunnan Han nationality, while HLA-DQB1*0201 may be the protective gene of PLE in Yunnan Han nationality.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R758.14
本文編號：2379145
[Abstract]:Objective to investigate the relationship between (Polymorphic light eruption,PLE and HLA-DQA1,DQB1 alleles and to identify the possible HLA susceptibility alleles and / or protective genes in PLE. Methods Genomic DNA, was extracted from peripheral venous leukocytes of 110 patients with PLE and 170 healthy controls by classical phenol-chloroform method. Polymerase chain reaction-direct sequencing (polymerase chain reaction sequence-based typing, was used. PCR-SBT was used to detect HLA-DQA1,DQB1 alleles in PLE patients and healthy controls. Results compared with the control group, HLA-DQA1*0102,DQB1*0302 and DQB1*0601 (OR=3.000,Pc=0.0045;OR=7.485,Pc10-3;) were found in the case group. The allele frequency of OR=14.153,Pc10-4 increased significantly, while the frequency of DQB1*0201 decreased significantly (OR=0.354,Pc=0.0280). Conclusion HLA-DQA1*0102,DQB1*0302 and DQB1*0601 may be susceptible genes of PLE or linked to actual susceptibility genes in Yunnan Han nationality, while HLA-DQB1*0201 may be the protective gene of PLE in Yunnan Han nationality.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R758.14
【參考文獻】
相關(guān)期刊論文 前3條
1 范麗安,周光炎;人類基因組MHC測序及其免疫學(xué)意義[J];上海免疫學(xué)雜志;1999年06期
2 鄧丹琪,韓云濤,陳浩,李海英;云南省不同地區(qū)多形性日光疹、慢性光線性皮炎患病率調(diào)查[J];中華皮膚科雜志;2005年07期
3 ;Polymorphism of the second exon of human leukocyte antigen-DQA1, -DQB1 gene and genetic susceptibility to idiopathic dilated cardiomyopathy in people of the Han nationality in northern China[J];Chinese Medical Journal;2005年03期
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