麻風(fēng)和HIV合并感染者IL-10、IL-12、CCR5、CXCR4表達(dá)研究
發(fā)布時(shí)間：2018-11-15 19:34
【摘要】：目的：初步探討麻風(fēng)病和HIV合并感染后機(jī)體免疫狀態(tài)的變化,為進(jìn)一步做好麻風(fēng)病和艾滋病的防治工作提供科學(xué)的依據(jù)。方法：收集麻風(fēng)和HIV合并感染者3例、HIV感染者9例、麻風(fēng)感染者10例、健康對(duì)照9例,采用流式細(xì)胞術(shù)檢測CD4+和CD8+T細(xì)胞表面趨化因子受體CCR5和CXCR4表達(dá)情況；雙抗體夾心ELISA方法檢測血漿細(xì)胞因子IL-10、IL-12的含量；實(shí)時(shí)熒光定量PCR(RT-PCR)方法檢測HIV病毒載量。結(jié)果：(1)麻風(fēng)和HIV合并感染組、HIV感染組CD4+T細(xì)胞絕對(duì)計(jì)數(shù)和CD4/CD8比值均顯著降低(p0.05),與麻風(fēng)和HIV合并感染組對(duì)比,HIV感染組CD4/CD8比值顯著降低(p0.05),進(jìn)一步對(duì)HIV感染組分層后分析無統(tǒng)計(jì)學(xué)意義(p0.05)。(2)麻風(fēng)和HIV合并感染組、HIV感染組CD4+T細(xì)胞表面的CCR5表達(dá)輕度升高,CD8+T細(xì)胞表面的CCR5表達(dá)輕度降低,但均無統(tǒng)計(jì)學(xué)意義(p0.05)；HIV感染組CD4+T細(xì)胞表面CCR5表達(dá)與病毒載量無相關(guān)性(r=0.119,p0.05)。(3)麻風(fēng)和HIV合并感染組、麻風(fēng)感染組、HIV感染組、健康對(duì)照組CD4+T細(xì)胞表面趨化因子受體CXCR4的表達(dá)無顯著性差異(p0.05)。(4)麻風(fēng)和HIV合并感染組、HIV感染組、麻風(fēng)感染組IL-10表達(dá)水平均升高,但是與健康對(duì)照組比較無統(tǒng)計(jì)學(xué)意義(p0.05)；HIV感染組血漿IL-10與CD4+T細(xì)胞CCR5表達(dá)呈正相關(guān)(r=0.762p=0.028)。(5)麻風(fēng)和HIV合并感染組、HIV感染組血漿IL-12水平均呈降低趨勢,但是無統(tǒng)計(jì)學(xué)意義(p0.05)。結(jié)論：(1)麻風(fēng)和HIV合并感染者、HIV感染者機(jī)體免疫功能減弱,與HIV感染者對(duì)比,麻風(fēng)和HIV合并感染者可能未出現(xiàn)更嚴(yán)重的免疫功能異常。(2)麻風(fēng)和HIV合并感染者CD4+T細(xì)胞表面CCR5高表達(dá),CXCR4低表達(dá),提示HIV感染可能是影響麻風(fēng)和HIV合并感染者CD4+T細(xì)胞表面CCR5和CXCR4表達(dá)的主要因素。(3)麻風(fēng)和HIV合并感染者、HIV感染者血漿IL-10輕度升高,IL-12水平降低,盡管與健康者對(duì)照無統(tǒng)計(jì)學(xué)意義,仍可初步說明兩組感染者Th0細(xì)胞向Th1細(xì)胞優(yōu)勢分化能力減弱,存在Th1/Th2平衡向Th2偏移的趨勢。(4)HIV感染者血漿IL-10與CD4+T細(xì)胞表面CCR5表達(dá)呈正相關(guān)。(5)本實(shí)驗(yàn)研究麻風(fēng)和HIV合并感染組樣本量較小,結(jié)果有待擴(kuò)大樣本含量進(jìn)一步探討。
[Abstract]:Objective: to explore the changes of immune status after leprosy and HIV infection in order to provide scientific basis for further prevention and control of leprosy and AIDS. Methods: the expression of chemokine receptor CCR5 and CXCR4 on CD4 and CD8 T cells was detected by flow cytometry in 3 cases of leprosy and HIV infection, 9 cases of HIV infection, 10 cases of leprosy infection and 9 cases of healthy control. The content of plasma cytokine IL-10,IL-12 was detected by double antibody sandwich ELISA and the viral load of HIV was detected by real-time fluorescence quantitative PCR (RT-PCR). Results: (1) in leprosy and HIV co-infection group, CD4 T cell absolute count and CD4/CD8 ratio were significantly decreased in HIV infection group (p0.05). Compared with leprosy and HIV co-infection group, CD4/CD8 ratio in HIV infected group was significantly lower (p0.05). After stratification of HIV infection group, there was no significant difference between leprosy and HIV co-infection group (p0.05). (2). In HIV infected group, CCR5 expression on CD4 T cell surface was slightly increased, CCR5 expression on CD8 T cell surface was slightly decreased. But there was no statistical significance (p0.05). There was no correlation between the expression of CCR5 on the surface of CD4 T cells and viral load in HIV infected group (r = 0.119 p0.05). (3), among leprosy and HIV co-infection group, leprosy infection group and HIV infection group. There was no significant difference in the expression of chemokine receptor CXCR4 on the surface of CD4 T cells in healthy control group (p0.05). (4). The expression of IL-10 in leprosy and HIV co-infection group, HIV infection group and leprosy infection group were all increased. But there was no significant difference compared with the healthy control group (p0.05). There was a positive correlation between plasma IL-10 and CD4 T cell CCR5 expression in HIV infection group (r=0.762p=0.028). (5) and HIV co-infection group. The plasma IL-12 level in HIV infected group was decreased, but there was no significant difference (p0.05). Conclusion: (1) in patients with leprosy and HIV infection, the immune function of HIV infected patients was weakened, compared with that of HIV infected patients. Leprosy and HIV co-infected patients may not have more serious immune dysfunction. (2) the expression of CCR5 on CD4 T cells was high and CXCR4 was low in leprosy and HIV co-infected patients. The results suggest that HIV infection may be the main factor affecting the expression of CCR5 and CXCR4 on CD4 T cells in patients with leprosy and HIV co-infection. (3) in patients with leprosy and HIV co-infection, the plasma IL-10 level is slightly higher and IL-12 level is lower in HIV infected patients. Although there was no significant difference between the two groups in comparison with the healthy controls, the ability of Th0 cells in the two groups to differentiate into Th1 cells was decreased. There was a tendency of Th1/Th2 balance shifting to Th2. (4) there was a positive correlation between plasma IL-10 and CCR5 expression on CD4 T cells in HIV infected patients. (5) the sample size of leprosy and HIV co-infection group was small. Results the sample size should be expanded further.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R755;R512.91
[Abstract]:Objective: to explore the changes of immune status after leprosy and HIV infection in order to provide scientific basis for further prevention and control of leprosy and AIDS. Methods: the expression of chemokine receptor CCR5 and CXCR4 on CD4 and CD8 T cells was detected by flow cytometry in 3 cases of leprosy and HIV infection, 9 cases of HIV infection, 10 cases of leprosy infection and 9 cases of healthy control. The content of plasma cytokine IL-10,IL-12 was detected by double antibody sandwich ELISA and the viral load of HIV was detected by real-time fluorescence quantitative PCR (RT-PCR). Results: (1) in leprosy and HIV co-infection group, CD4 T cell absolute count and CD4/CD8 ratio were significantly decreased in HIV infection group (p0.05). Compared with leprosy and HIV co-infection group, CD4/CD8 ratio in HIV infected group was significantly lower (p0.05). After stratification of HIV infection group, there was no significant difference between leprosy and HIV co-infection group (p0.05). (2). In HIV infected group, CCR5 expression on CD4 T cell surface was slightly increased, CCR5 expression on CD8 T cell surface was slightly decreased. But there was no statistical significance (p0.05). There was no correlation between the expression of CCR5 on the surface of CD4 T cells and viral load in HIV infected group (r = 0.119 p0.05). (3), among leprosy and HIV co-infection group, leprosy infection group and HIV infection group. There was no significant difference in the expression of chemokine receptor CXCR4 on the surface of CD4 T cells in healthy control group (p0.05). (4). The expression of IL-10 in leprosy and HIV co-infection group, HIV infection group and leprosy infection group were all increased. But there was no significant difference compared with the healthy control group (p0.05). There was a positive correlation between plasma IL-10 and CD4 T cell CCR5 expression in HIV infection group (r=0.762p=0.028). (5) and HIV co-infection group. The plasma IL-12 level in HIV infected group was decreased, but there was no significant difference (p0.05). Conclusion: (1) in patients with leprosy and HIV infection, the immune function of HIV infected patients was weakened, compared with that of HIV infected patients. Leprosy and HIV co-infected patients may not have more serious immune dysfunction. (2) the expression of CCR5 on CD4 T cells was high and CXCR4 was low in leprosy and HIV co-infected patients. The results suggest that HIV infection may be the main factor affecting the expression of CCR5 and CXCR4 on CD4 T cells in patients with leprosy and HIV co-infection. (3) in patients with leprosy and HIV co-infection, the plasma IL-10 level is slightly higher and IL-12 level is lower in HIV infected patients. Although there was no significant difference between the two groups in comparison with the healthy controls, the ability of Th0 cells in the two groups to differentiate into Th1 cells was decreased. There was a tendency of Th1/Th2 balance shifting to Th2. (4) there was a positive correlation between plasma IL-10 and CCR5 expression on CD4 T cells in HIV infected patients. (5) the sample size of leprosy and HIV co-infection group was small. Results the sample size should be expanded further.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R755;R512.91
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相關(guān)期刊論文 前3條
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