【摘要】：目的研究牽張力作用下瘢痕疙瘩間充質(zhì)干細(xì)胞(keloid-derived mesenchymal stem cells,KD-MSCs)培養(yǎng)上清對瘢痕疙瘩成纖維細(xì)胞(keloid fibroblasts,KFs)增殖和膠原合成的影響,探討牽張力及KD-MSCs在瘢痕疙瘩形成過程中的作用。方法培養(yǎng)原代KD-MSCs和KFs,于72h收集牽張力作用下和未施加牽張力的KD-MSCs培養(yǎng)上清,并分別孵育KFs 24h,48h,72h,96h,倒置相差顯微鏡觀察細(xì)胞的形態(tài)學(xué)變化,CCK-8法檢測KFs的增殖活力,Real-time PCR檢測KFs中Ⅰ型和Ⅲ型膠原和CTGF mRNA轉(zhuǎn)錄情況,羥脯氨酸消化法檢測膠原水平的變化。結(jié)果培養(yǎng)上清刺激后,兩組間KFs形態(tài)未見明顯差異;CCK-8結(jié)果提示牽張力組KFs增殖明顯強(qiáng)于無牽張力組(P0.05);Real-time PCR結(jié)果示牽張力組KFsⅠ型膠原的mRNA表達(dá)明顯高于無牽張力組(P0.01),牽張力組KFsⅢ型膠原的mRNA表達(dá)低于無牽張力組(P0.05),牽張力組KFs CTGF的mRNA表達(dá)明顯低于無牽張力組(P0.01);羥脯氨酸檢測提示牽張力組KFs羥脯氨酸含量明顯高于無牽張力組(P0.05)。結(jié)論牽張力作用可明顯增強(qiáng)KD-MSCs培養(yǎng)上清對KFs增殖和膠原合成的促進(jìn)作用,提示牽張力作用下KD-MSCs可發(fā)生旁分泌促進(jìn)成纖維細(xì)胞增生和分泌,從而參與瘢痕疙瘩的形成,其具體機(jī)制仍需深入研究。
[Abstract]:Objective to study the effects of the culture supernatant of keloid mesenchymal stem cells (keloid-derived mesenchymal stem cells,KD-MSCs) on the proliferation and collagen synthesis of keloid fibroblasts (keloid fibroblasts,KFs) and the role of KD-MSCs in the process of keloid formation. Methods the supernatants of primary KD-MSCs and KFs, were collected under and without stretch for 72 h. The morphological changes of cells were observed by inverted phase contrast microscope. The proliferative activity of KFs was detected by CCK-8 method Real-time PCR was used to detect the transcription of type 鈪，

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