【摘要】：目的：通過對2%酮康唑乳膏、2%硝酸舍他康唑乳膏、1%聯(lián)苯芐唑乳膏的體外藥敏實驗分析3種咪唑類抗真菌藥物的體外抗真菌活性。從而為這3種藥物的臨床使用提供依據(jù)，指導(dǎo)臨床用藥。 方法：選用臨床分離致病真菌共5種50株，用瓊脂擴散法進行體外藥敏實驗。制備含2%瓊脂的馬鈴薯瓊脂含菌培養(yǎng)基，將3種藥物等體積的打入含菌平板的加藥孔。28℃恒溫培養(yǎng)10天后測量每種藥周圍的抑菌環(huán)半徑，綜合數(shù)據(jù)進行統(tǒng)計學(xué)分析。 結(jié)果：2%酮康唑乳膏、2%硝酸舍他康唑乳膏、1%聯(lián)苯芐唑乳膏對3種皮膚癬菌菌株40株、2種念珠菌菌株10株共5種50株菌株均形成抑菌圈。2%酮康唑乳膏的抑菌圈半徑均數(shù)為13.72mm，2%硝酸舍他康唑乳膏的抑菌圈半徑均數(shù)為12.35mm、1%聯(lián)苯芐唑乳膏的抑菌圈半徑均數(shù)為6.94mm。 結(jié)論：通過對2%酮康唑乳膏、2%硝酸舍他康唑乳膏、1%聯(lián)苯芐唑乳膏的體外藥敏實驗，得出對于所有實驗菌株，3種抗真菌藥物的抗真菌活性均有統(tǒng)計學(xué)差異，抗真菌活性由強到弱依次為：2%酮康唑乳膏2%硝酸舍他康唑乳膏1%聯(lián)苯芐唑乳膏。對于皮膚癬菌3種藥的抗真菌活性為：2%酮康唑乳膏2%硝酸舍他康唑乳膏1%聯(lián)苯芐唑乳膏；對于念珠菌3種藥的抗真菌活性為：2%酮康唑乳膏與2%舍他康唑乳膏差異無統(tǒng)計學(xué)意義，，但二者均優(yōu)于1%聯(lián)苯芐唑乳膏。
[Abstract]:Aim: to analyze the in vitro antifungal activity of three imidazole antifungal agents by in vitro drug sensitivity test of 2% ketoconazole cream and 2% seraconazole nitrate cream and 1% bifonazole cream. So as to provide the basis for the clinical use of these three drugs, guiding clinical use. Methods: 50 strains of 5 kinds of pathogenic fungi were isolated and tested by Agar diffusion in vitro. Potato Agar culture medium containing 2% Agar was prepared. After 10 days of constant temperature culture, the radius of bacteriostasis ring around each drug was measured, and the comprehensive data were analyzed statistically. Results 2% 2% ketoconazole cream and 2% sertaconazole nitrate 1% bifonazole cream formed inhibition circle radius of 3 strains of dermatophytes 40 strains, 2 strains of Candida, 10 strains of 5 species of 50 strains. 2% ketoconazole cream. 2% ketoconazole cream, 2% ketoconazole cream. The mean radius of bacteriostasis circle was 12.35 mm ~ (-1) bifonazole cream with a mean of 13.72 mm ~ (-2) nitrateconazole nitrate and 6.94 mm ~ (-1) bifonazole cream. Conclusion: the in vitro drug sensitivity test of 2% ketoconazole cream and 2% seraconazole nitrate cream and 1% bifonazole cream showed that there were significant differences in the antifungal activities among the three antifungal drugs against all experimental strains. The order of antifungal activity from strong to weak is: 1% bifonazole cream 2% ketoconazole cream 2% sertaconazole nitrate cream 1% bifonazole cream. The antifungal activity of these three drugs was 1% bifonazole cream, 2% ketoconazole cream, 2% saltaconazole nitrate cream, 1% bifonazole cream, 2% ketoconazole cream, 2% oxaconazole cream, 1% bifonazole cream. There was no significant difference in the antifungal activity of 2% ketoconazole cream and 2% sertaconazole cream against Candida, but both of them were superior to 1% bifonazole cream.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R756

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