本文選題：FICZ + AHR��； 參考：《應(yīng)用激光》2017年05期

【摘要】：研究紫外線誘導(dǎo)的光產(chǎn)物FICZ(6-Formylindolo[3,2-b]carbazole)對體外培養(yǎng)的人皮脂腺細(xì)胞芳香烴受體(AHR)信號通路影響及相關(guān)炎癥因子mRNA表達(dá)的影響,探討紫外線誘導(dǎo)痤瘡發(fā)生的機制。方法:Real-time qPCR檢測分析永生化的SZ95人皮脂腺細(xì)胞暴露于紫外線光產(chǎn)物FICZ后,檢測AHR及其下游基因CYP1A1表達(dá),以及炎癥基因IL-1α、IL-1β、RELA等表達(dá)。結(jié)果:FICZ組AHR表達(dá)豐度是CONTROL組(未加入FICZ組,以下均稱對照組)的1.412倍(P0.01);FICZ組CYP1A1基因表達(dá)豐度是對照組的142.338倍(P0.01);FICZ組IL-1α基因表達(dá)豐度是對照組的4.039倍(P0.01);FICZ組IL-1β基因表達(dá)豐度是對照組的4.245倍(P0.01);FICZ組RELA基因表達(dá)豐度是對照組的1.432倍(P0.01)。結(jié)論:紫外線光產(chǎn)物FICZ激活A(yù)HR信號通路,從而影響皮脂腺細(xì)胞的炎癥因子IL-1α、IL-1β、RELA等產(chǎn)生,這可能是紫外線誘導(dǎo)痤瘡發(fā)生的機制之一。
[Abstract]:To investigate the effect of UV-induced light product FICZ (6-Formylindolo [32-b] carbazole) on the signal pathway of aromatic hydrocarbon receptor (AHR) and the expression of related inflammatory factors in cultured human sebaceous gland cells, and to explore the mechanism of UV-induced acne. Methods the expression of AHR and its downstream gene CYP1A1, and the inflammatory gene IL-1 偽, IL-1 尾 -rela, were detected in SZ95 human sebaceous gland cells exposed to ultraviolet light product (FICZ) by 1: Real-time qPCR. Results the expression abundance of AHR in FICZ group was Contral group (FICZ group was not added to FICZ group). The abundance of CYP1A1 gene expression in FICZ group was 142.338 times higher than that in control group (P0.01). The abundance of IL-1 尾 gene expression in FICZ group was 4.039 times higher than that in control group (P0.01). The abundance of IL-1 尾 gene expression in FICZ group was 4.245 times higher than that in FICZ group (P0.01), and the abundance of rela gene in FICZ group was 1.432 times higher than that in control group (P0.01). Conclusion: ultraviolet light product FICZ activates the AHR signaling pathway, thus affecting the production of IL-1 偽, IL-1 尾 -triarela and so on in sebaceous gland cells, which may be one of the mechanisms of UV induced acne.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬仁濟醫(yī)院皮膚科;德國德紹醫(yī)學(xué)中心皮膚性病和變態(tài)反應(yīng)免疫科;
【基金】：國家自然科學(xué)基金資助項目(項目編號:81472894);國家自然科學(xué)基金資助項目(項目編號:81602772) 上海市自然科學(xué)基金資助項目(項目編號:14ZR1424900)
【分類號】：R758.733

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