本文選題：梅毒 + TP15　； 參考：《山西醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的比較性研究一期,二期、潛伏梅毒及血清固定型梅毒患者對(duì)梅毒螺旋體外膜脂蛋白TP15、TP17、TP47抗原特異性體液免疫和細(xì)胞免疫反應(yīng)。了解梅毒螺旋體外膜脂蛋白TP15,、TP17、TP47抗原在梅毒特異性細(xì)胞免疫和體液免疫中的作用。 方法采集正常對(duì)照者、一期梅毒、二期梅毒、潛伏梅毒患者以及治療后兩年RPR未轉(zhuǎn)陰血清固定型梅毒患者的外周血,分離血清和外周血單個(gè)核細(xì)胞,將梅毒螺旋體TP15、TP17、TP47蛋白作為特異性梅毒抗原,采用immuno-slot blot method方法檢測(cè)梅毒患者血清中TP15、TP17和TP47特異性抗體狀況。通過酶聯(lián)免疫斑點(diǎn)技術(shù)(enzyme-linked immuno-spot, Elispot)檢測(cè)梅毒患者中TP15、TP17和TP47刺激細(xì)胞產(chǎn)生細(xì)胞因子(INF-γ)。 結(jié)果本試驗(yàn)通過immun-solt bolt方法對(duì)8例一期梅毒,15例二期梅毒,15例潛伏梅毒和13例血清固定型梅毒患者TP15、TP17和TP47進(jìn)行檢測(cè),結(jié)果：一期梅毒中TP15的陽性率約為25%,TP17為100%,TP47為87.5%。TP15與TP17間χ2=4.17,P0.05,有統(tǒng)計(jì)學(xué)意義；TP15與TP47間χ2=4.0,P0.05,存在統(tǒng)計(jì)學(xué)差異；TP17與TP47之間χ2=0.00,P0.05,差異無顯著性；二期梅毒中,TP15陽性率為80%,TP17為100%,TP15與TP17和TP47間χ2=1.75,P0.05,無統(tǒng)計(jì)學(xué)差異。TP47的陽性率的檢出率也很高,約為93.4%。各組別間陽性率比較較高的為二期梅毒。本研究利用ELISPOT技術(shù)檢測(cè)三種梅毒外膜脂蛋白,結(jié)果：與正常對(duì)照組相比,二期梅毒各抗原刺激的差異均有顯著性,一期、二期和血清固定型梅毒針對(duì)TP17抗原產(chǎn)生的細(xì)胞因子INF-γ與正常對(duì)照組相比存在統(tǒng)計(jì)學(xué)差異,潛伏梅毒對(duì)三種抗原產(chǎn)生的INF-γ與正常對(duì)照組相比均無統(tǒng)計(jì)學(xué)差異。 結(jié)論用TP15、TP17、TP47三種重組蛋白作為抗原刺激物通過,Immun-solt blod技術(shù)對(duì)各期梅毒進(jìn)行體液免疫檢測(cè),Elispot技術(shù)進(jìn)行細(xì)胞免疫檢測(cè),發(fā)現(xiàn)這三種抗原可引起廣泛的特異性體液免疫,而特異性細(xì)胞免疫反應(yīng)相對(duì)較弱。為梅毒患者在發(fā)病中的免疫變化情況提供實(shí)驗(yàn)室依據(jù),為探討梅毒的發(fā)病機(jī)制提供新的思路。
[Abstract]:Objective to compare the specific humoral and cellular immune responses of patients with latent syphilis and serum-fixed syphilis to TP15 TP17 TP47 antigen of Treponema pallidum. Objective: to investigate the role of TP17 / TP47 antigen of Treponema pallidum outer membrane lipoprotein TP15 in specific cellular and humoral immunity of syphilis. Methods Peripheral blood samples were collected from normal controls, primary syphilis, secondary syphilis, latent syphilis, and two years after treatment with RPR non-negative serum-fixed syphilis, and the serum and peripheral blood mononuclear cells were isolated. Treponema pallidum TP15, TP17, TP47 were used as specific syphilis antigens, and the specific antibodies of TP15, TP17 and TP47 in serum of syphilis patients were detected by immuno-slot blot method. TP15, TP17 and TP47 stimulated cytokines (INF- 緯) were detected by enzyme-linked immuno spot (Elispot) in patients with syphilis. Results TP15, TP17 and TP47 were detected by immun-solt bolt in 8 cases of primary syphilis and 15 cases of secondary syphilis and 13 cases of serum fixed syphilis. Results: the positive rate of TP15 in primary syphilis was about 25%. TP17 was 100 and TP47 was 87.5.TP15 and TP17 were 87.5.TP15 and TP17 蠂 24.17g / P0.05. there was statistical significance between TP15 and TP47 (蠂 24.0 P0.05), there was no significant difference between TP17 and TP47 (蠂 20.00P 0.05). In secondary syphilis, the positive rate of TP15 was 80% and TP17 was 100%. There was no statistical difference between TP15 and TP17 and TP47. The positive rate of TP15 was also very high, about 93.4. The higher positive rate of syphilis in each group was secondary syphilis. In this study, three kinds of syphilis outer membrane lipoproteins were detected by Elispot technique. Results: compared with the normal control group, there were significant differences in each antigen stimulation of secondary syphilis. The levels of cytokine INF- 緯 against TP17 antigen in secondary and serum stationary syphilis were significantly different from those in normal control group, but there was no significant difference between latent syphilis and normal control group. Conclusion the three recombinant proteins of TP15, TP17 and TP47 were used as antigenic stimulators to detect the cellular immunity of syphilis by immuno-immunoassay with Immun-solt blod. It was found that these three antigens could induce a wide range of specific humoral immunity. The specific cellular immune response was relatively weak. To provide laboratory basis for the immune changes of syphilis patients, and to provide a new way to explore the pathogenesis of syphilis.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R759.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 陳宏翔,盛晚香,宋繼權(quán);梅毒病程中宿主的體液免疫學(xué)變化探討[J];中國麻風(fēng)皮膚病雜志;2003年02期

2 張錫寶,韓爾陽,湯少開,李季,陸美英,許斌;廣州市梅毒流行病學(xué)十年回顧性分析[J];現(xiàn)代預(yù)防醫(yī)學(xué);2002年02期

，

本文編號(hào)：2066919