本文選題：真皮多能干細(xì)胞 + 皮膚光老化�。� 參考：《遵義醫(yī)學(xué)院》2010年碩士論文

【摘要】： 目的：本研究擬探討真皮多能干細(xì)胞(dermal multipotential stem cells, DMSCs)移植對(duì)大鼠皮膚光老化的修復(fù)作用,為皮膚光老化提供新的治療方法。方法：57只SD大鼠隨機(jī)分為正常組8只和模型組49,采用UVA和UVB紫外燈同時(shí)對(duì)模型組進(jìn)行照射(每天2h,連續(xù)照射60天),隨機(jī)取8只大鼠進(jìn)行皮膚光老化模型鑒定,模型成功后隨機(jī)分為對(duì)照組16只(PBS代替)和治療組25只(1×106細(xì)胞懸液)。體外分離培養(yǎng)SD乳鼠DMSCs,經(jīng)鑒定后用DAPI標(biāo)記,取治療組大鼠9只,皮膚光老化區(qū)多點(diǎn)真皮層內(nèi)移植細(xì)胞懸液,分別在移植后1d、3d、7d處死大鼠各3只,在熒光顯微鏡下觀察DAPI標(biāo)記的DMSCs在皮膚光老化區(qū)域組織存活、遷徙和定居。然后對(duì)剩余治療組大鼠皮膚光老化區(qū)進(jìn)行細(xì)胞移植；4周、8周分別采用免疫組織化學(xué)(波形蛋白)、VG和Weigert染色方法檢測(cè)各組真皮中成纖維細(xì)胞、膠原纖維和彈力纖維的變化。結(jié)果：在不同時(shí)間段檢測(cè)到DAPI標(biāo)記的DMSCs在皮膚光老化區(qū)存活、擴(kuò)散、遷移。免疫組織化學(xué)染色結(jié)果顯示：與對(duì)照組相比：4周后治療組成纖維細(xì)胞增多,主要在真皮淺層較多,兩者比較其差異有統(tǒng)計(jì)學(xué)意義(P0.01),8周后廣泛均勻彌散在皮膚各層,兩者比較其差異有統(tǒng)計(jì)學(xué)意義(P0.01)。VG染色結(jié)果顯示：與對(duì)照組相比：4周后治療組變性、成塊膠原纖維含量減少,可見(jiàn)少量新生膠原纖維,8周后延伸至皮下組織及真皮深層,變性膠原纖維基本消失,出現(xiàn)大量染色深、排列有序的新生膠原纖維,皮膚結(jié)構(gòu)層次清晰,與正常組無(wú)明顯變化。Weigert染色結(jié)果顯示：與對(duì)照組相比：4周后治療組真皮深層聚集、纏結(jié)的變性彈力纖維明顯減少,8周后真皮淺層變性彈力纖維基本消失,毛囊周圍及真皮淺層見(jiàn)纖細(xì)新生波浪狀彈力纖維,與正常組無(wú)明顯變化。結(jié)論：DMSCs移植對(duì)大鼠皮膚光老化有明顯的治療作用。
[Abstract]:Objective: to investigate the effect of dermal pluripotent stem cell transplantation on photoaging of rat skin, and to provide a new therapeutic method for skin photoaging. Methods Fifty-seven Sprague-Dawley rats were randomly divided into normal group (n = 8) and model group (n = 49). The model group was irradiated with UVA and UVB ultraviolet lamp at the same time. After success, the model was randomly divided into control group (n = 16) and treatment group (n = 25). SD neonatal rats were isolated and cultured in vitro. After identification, 9 rats in the treatment group were labeled with DAPI, and 3 rats in each group were killed at 1 day after transplantation, 3 rats were killed on the 3rd day after transplantation. DAPI labeled DMSCs were observed to survive, migrate and settle in skin photoaging area under fluorescence microscope. The changes of fibroblasts, collagen fibers and elastic fibers in the dermis of the remaining treatment group were detected by immunohistochemical staining (vimentin VG and Weigert staining) after 4 weeks and 8 weeks of cell transplantation. Results: DAPI labeled DMSCs were detected to survive, diffuse and migrate in skin photoaging area at different time points. The results of immunohistochemical staining showed that compared with the control group, the number of fibroblasts increased after 4 weeks of treatment, mainly in the superficial dermis. The difference between the two groups was statistically significant after 8 weeks. The difference between the two groups was statistically significant (P 0.01). VG staining showed that compared with the control group, the denaturation of collagen fibers in the treatment group decreased after 4 weeks compared with the control group. It was found that a small amount of new collagen fibers extended to the subcutaneous tissue and the deep dermis after 8 weeks. The denatured collagen fibers disappeared, a large number of new collagen fibers were stained deeply, the skin structure was clear and the skin structure was clear. The results of Weigert staining showed that: compared with the control group, the treatment group had deep dermal aggregation after 4 weeks. The denatured elastic fibers of tangles decreased significantly after 8 weeks, the denatured elastic fibers in the superficial dermis disappeared basically, and the new wave elastic fibers were found around the hair follicles and in the superficial layers of the dermis, which had no obvious change from the normal group. Conclusion\
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R758.1

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