本文選題：黑色素細(xì)胞瘤 + 樹突細(xì)胞��； 參考：《中國老年學(xué)雜志》2014年09期

【摘要】：目的探討自然調(diào)節(jié)性細(xì)胞(nTreg)對基于樹突狀細(xì)胞(DC)和細(xì)胞因子誘導(dǎo)的殺傷細(xì)胞(CIK)細(xì)胞疫苗治療黑色素細(xì)胞瘤小鼠療效的影響。方法制備出小鼠的黑色素瘤動物模型40只。制備刪除nTreg的CIK細(xì)胞、未刪除nTreg的CIK細(xì)胞及輸入DC和CIK細(xì)胞后再去除nTreg的細(xì)胞,并將各組細(xì)胞輸入動物模型內(nèi),觀察各組細(xì)胞體外殺傷活性及動物模型生存情況。結(jié)果第1組制備的DC和CIK疫苗殺傷黑色素瘤細(xì)胞活性最強(qiáng),平均殺傷活性為(98.32±6.24)%,顯著高于第2組(45.53±9.13)%和第3組(51.27±8.24)%(P0.05)。對照組小鼠腫瘤體積顯著升高,治療B和C組在第8、12天腫瘤生長被抑制,治療A組在第6天腫瘤被抑制,第8、12天腫瘤大小顯著降低。對照組小鼠在30 d內(nèi)全部死亡,治療A組僅在第3、6天分別死亡1只,治療B組30 d內(nèi)有5只存活,治療C組30 d有3只存活,均顯著高于對照組(均P0.05)。結(jié)論 nTreg會抑制DC和CIK疫苗殺傷腫瘤細(xì)胞活性以及治療腫瘤療效。
[Abstract]:Objective to investigate the effect of natural regulatory cells (nTreg) on the therapeutic effect of dendritic cells (DC) and cytokine induced killer cell (CIK) cell vaccine on melanocytoma mice. Methods 40 mouse melanoma models were prepared. CIK cells deleted from nTreg, CIK cells of nTreg, DC and CIK fine were not deleted. The cells of nTreg were removed and the cells of each group were entered into animal models. The killing activity of the cells in vitro and the survival of the animal models were observed. The results showed that the first groups of DC and CIK vaccines killed the melanoma cells with the strongest activity, and the average killing activity was (98.32 + 6.24)%, which was significantly higher than that of the second groups (45.53 + 9.13)% and third groups (51.27 + 8.24). The tumor volume in the control group was significantly higher than that in the control group. The tumor growth was suppressed in the B and C groups on day 8,12. The tumor was suppressed in the A group at sixth days and the size of the tumor decreased significantly on the 8,12 day. The control group died in the 30 d and the A group died only 1 in the 3,6 day, 5 in the B group 30 d, and 3 in the 30 C group 3. 3 were treated C group 30 3. 3 were treated C group 30 3 The survival rate was significantly higher than that of the control group (all P0.05). Conclusion nTreg can inhibit the activity of DC and CIK vaccine in killing tumor cells and the therapeutic effect of tumor.
【作者單位】： 河北醫(yī)科大學(xué)第二醫(yī)院肛腸外科;河北醫(yī)科大學(xué)第二醫(yī)院心電圖室;
【基金】：河北省衛(wèi)生廳科研基金項(xiàng)目(No.20090087)
【分類號】：R739.5

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