本文選題：變態(tài)反應(yīng)性疾病 + 免疫印跡法��； 參考：《中國(guó)醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的 分析四種變態(tài)反應(yīng)性疾病患者血清總IgE和過(guò)敏原特異性IgE (sIgE)檢測(cè)結(jié)果,為更好地診斷、治療和預(yù)防過(guò)敏性疾病提供可靠的科學(xué)依據(jù)和參考。 方法 選取2008年10月至2010年1月年中國(guó)醫(yī)科大學(xué)附屬第一醫(yī)院皮膚科就診的412例變態(tài)反應(yīng)性疾病患者,入選患者分別診斷為濕疹、蕁麻疹、特應(yīng)性皮炎和過(guò)敏性鼻炎。采用免疫印跡定量過(guò)敏原檢測(cè)系統(tǒng)對(duì)患者血清總IgE和過(guò)敏原sIgE進(jìn)行檢測(cè),并采用統(tǒng)一設(shè)計(jì)的臨床問(wèn)卷,詳細(xì)記錄患者性別、年齡、發(fā)病時(shí)間、皮損形態(tài)及分布、用藥史等情況,應(yīng)用SPSS16.0軟件包對(duì)不同疾病組血清總IgE和過(guò)敏原sIgE檢測(cè)結(jié)果進(jìn)行分析。 結(jié)果 檢測(cè)412例變態(tài)反應(yīng)性疾病患者和69例正常對(duì)照者的血清總IgE和過(guò)敏原sIgE,其結(jié)果如下： 1、412例變態(tài)反應(yīng)疾病患者中,370例血清總IgE陽(yáng)性,陽(yáng)性率為89.8%；蕁麻疹、濕疹、特應(yīng)性皮炎及過(guò)敏性鼻炎這四種過(guò)敏性疾病總IgE陽(yáng)性率不同,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。 2、412例變態(tài)反應(yīng)疾病患者中,226例血清sIgE陽(yáng)性,陽(yáng)性率為54.9%。這四種變態(tài)反應(yīng)性疾病sIgE陽(yáng)性率高于正常對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義(P0.001)。其中,對(duì)一種過(guò)敏原sIgE反應(yīng)陽(yáng)性119例,以特應(yīng)性皮炎最高,占同種疾病陽(yáng)性反應(yīng)的46.2%；對(duì)兩種過(guò)敏原陽(yáng)性反應(yīng)62例,特應(yīng)性皮炎最為明顯,占同種疾病陽(yáng)性反應(yīng)的26.9%；對(duì)三種及三種以上過(guò)敏原檢測(cè)陽(yáng)性45例,以過(guò)敏性鼻炎為著,占同種疾病陽(yáng)性反應(yīng)的40%。 3、412例變態(tài)反應(yīng)性疾病中,吸入性過(guò)敏原sIgE檢出陽(yáng)性率較食入性過(guò)敏原高,分別為65.3%和34.7%。其中,吸入組檢出陽(yáng)性率前4位分別是貓狗毛皮屑(16.3%)、戶塵螨粉塵螨(13.4%)、蟑螂(10.1%)和多種霉菌(9.2%)。食入組中前3位分別是小麥(9.4%)、牛肉羊肉(8.1%)和魚(yú)蝦蟹(4.2%)。 4、慢性蕁麻疹患者血清總IgE和過(guò)敏原sIgE同時(shí)陽(yáng)性率高于急性蕁麻疹,差異具有統(tǒng)計(jì)學(xué)意義(P0.001)；急慢性蕁麻疹患者中一種過(guò)敏原、兩種過(guò)敏原和三種及三種以上過(guò)敏原sIgE檢出陽(yáng)性率不同,差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)；急慢性蕁麻疹中不同種類過(guò)敏原sIgE的檢測(cè)結(jié)果大致相同,但慢性蕁麻疹患者中樹(shù)木花粉、腰果花生黃豆和小麥sIgE陽(yáng)性率較高,分別為7.2%、3.6%、7.8%。 結(jié)論 1、在蕁麻疹、濕疹、特應(yīng)性皮炎及過(guò)敏性鼻炎這四種變態(tài)反應(yīng)性疾病中,總IgE檢測(cè)陽(yáng)性率不同,以蕁麻疹最高,為93.3%。 2、蕁麻疹、濕疹、特應(yīng)性皮炎及過(guò)敏性鼻炎患者sIgE陽(yáng)性率均高于正常對(duì)照組；其中吸入性過(guò)敏原sIgE陽(yáng)性率高于食入性過(guò)敏原,吸入性過(guò)敏原主要為貓狗毛皮屑、戶塵螨粉塵螨、蟑螂及多種霉菌,食入性過(guò)敏原主要為小麥、牛肉羊肉和魚(yú)蝦蟹。 3、與急性蕁麻疹相比,慢性蕁麻疹患者血清總IgE和過(guò)敏原sIgE同時(shí)陽(yáng)性率較高,同時(shí)較易存在多種過(guò)敏原sIgE陽(yáng)性；慢性蕁麻疹患者中樹(shù)木花粉、腰果花生黃豆和小麥sIgE陽(yáng)性率高于急性蕁麻疹。
[Abstract]:Objective to analyze the results of serum total IgE and allergen specific IgE (IgE) in patients with four allergic diseases so as to provide reliable scientific basis and reference for better diagnosis, treatment and prevention of allergic diseases. Methods 412 patients with allergic diseases were selected from the dermatology department of the first affiliated Hospital of China Medical University from October 2008 to January 2010. The patients were diagnosed as eczema and urticaria respectively. Atopic dermatitis and allergic rhinitis. Serum total IgE and allergen Siga E were detected by Western blot quantitative allergen detection system. The patients' gender, age, onset time, skin lesion morphology and distribution, drug history and so on were recorded by a unified clinical questionnaire. The results of serum total IgE and allergen sIgE in different disease groups were analyzed by SPSS 16.0 software package. Results Serum total IgE and allergen sIgE were measured in 412 patients with allergic diseases and 69 normal controls. The results were as follows: 1412 patients with allergic diseases were positive for serum total IgE. The total positive rate of IgE in urticaria, eczema, atopic dermatitis and allergic rhinitis was different, and the difference was statistically significant (P 0.05). The positive rate was 54.9%. The sIgE positive rate of these four allergic diseases was higher than that of the normal control group, and the difference was statistically significant (P 0.001). Among the 119 cases with positive Siga E reaction to one allergen, atopic dermatitis was the highest, accounting for 46.2% of the positive reaction for the same disease, while for 62 cases of the two allergens, atopic dermatitis was the most obvious, accounting for 26.9% of the positive reaction of the same disease. Among the 45 cases of allergic rhinitis detected by three or more allergens, it accounted for 40. 3412 cases of allergic diseases. The positive rate of Siga was higher than that of ingestion allergen, 65.3% and 34.7%, respectively. In the inhaled group, the first four positive rates were Cat and Dog hair dandruff (16.3C), Dust mites (13.4m), cockroach (10.1cm) and a variety of molds (9.2g). The positive rates of serum total IgE and allergen Siga in patients with chronic urticaria were significantly higher than those in patients with acute urticaria (P 0.001). The positive rates of one allergen, two allergens and three or more allergens in patients with acute and chronic urticaria were different, and the difference was not statistically significant (P 0.05). But the positive rates of tree pollen, cashew nut, soybean and wheat Siga were higher in patients with chronic urticaria. Conclusion 1. In urticaria, eczema, atopic dermatitis and allergic rhinitis, the positive rate of total IgE was different. The positive rate of Siga E in eczema, atopic dermatitis and allergic rhinitis was higher than that in normal control group, and the positive rate of inhaled allergen was higher than that of ingestion allergen. In cockroaches and many kinds of fungi, the main food allergens were wheat, beef and mutton, fish, shrimp and crab. 3. Compared with acute urticaria, the positive rates of serum total IgE and allergen Siga E in patients with chronic urticaria were higher than those in patients with chronic urticaria. The positive rate of tree pollen, cashew nut soybean and wheat Siga E in chronic urticaria was higher than that in acute urticaria.
【學(xué)位授予單位】：中國(guó)醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R758.24

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