本文選題：惡性黑色素瘤 + 慢性炎癥��； 參考：《天津醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的： 探索建立小鼠慢性炎癥-黑色素瘤復(fù)合模型的方法,通過觀察慢性炎癥對腫瘤體積、重量、微血管密度的影響,闡明慢性炎癥對進(jìn)展期移植腫瘤生長的影響,為進(jìn)一步研究炎癥因子對腫瘤生長的影響打下實驗學(xué)基礎(chǔ)。 內(nèi)容： 1.慢性炎癥模型的建立：在小鼠背部皮下注入空氣制作氣囊,向氣囊里注入致炎物質(zhì)脂多糖、卡介苗,觀察慢性炎癥模型造模效果以及炎癥由急性進(jìn)入慢性的時間段。 2.黑色素瘤模型的建立：75%酒精棉球消毒小鼠后,在鼠蹊部皮下注入黑色素瘤細(xì)胞懸浮液。 3.慢性炎癥-黑色素瘤復(fù)合模型的建立：在小鼠背部皮下注入空氣制作氣囊或包埋玻璃珠后,每周向氣囊里或玻璃珠周圍注入致炎物質(zhì)。初次注入致炎物質(zhì)3天后,在鼠蹊部皮下注入黑色素瘤細(xì)胞懸浮液建立四種小鼠慢性炎癥-黑色素瘤復(fù)合模型,比較優(yōu)劣,選擇合適的慢性炎癥-黑色素瘤復(fù)合模型。 4.慢性炎癥對黑色素瘤體積、重量和遠(yuǎn)處轉(zhuǎn)移的影響：建立慢性炎癥-黑色素瘤復(fù)合模型,比較對照組與實驗組腫瘤體積、重量的變化和肺、肝、脾的轉(zhuǎn)移發(fā)生率。 5.慢性炎癥對微血管密度的影響：通過HE染色比較對照組與實驗組微血管密度,明確內(nèi)皮依賴性血管在炎癥環(huán)境中的變化。 方法： 1.在小鼠背部皮下注入空氣制作氣囊,向氣囊里注入致炎物質(zhì)脂多糖、卡介苗,建立兩種慢性炎癥模型,觀察造模效果以及模型達(dá)到慢性炎癥狀態(tài)的時間段。 2.在前期實驗基礎(chǔ)上,在小鼠上分別建立氣囊卡介苗致炎腫瘤模型(A組),氣囊弗氏佐劑致炎腫瘤模型(B組),氣囊脂多糖致炎腫瘤模型(C組),埋珠脂多糖致炎腫瘤模型(D組),生理鹽水腫瘤模型為對照組(E組)。 3.計算各組腫瘤不同觀測時間點的體積,重量并進(jìn)行比較。HE染色,高倍光鏡下辨認(rèn)微血管,按Weidner法計數(shù)腫瘤組織樣本中微血管數(shù)；觀察腫瘤臟器轉(zhuǎn)移的發(fā)生率。 結(jié)果： 1.在小鼠背部皮下制作氣囊,注入脂多糖或卡介苗可以成功地建立小鼠皮下氣囊慢性炎癥模型。此模型能較好地模擬在單一致炎因子存在情況下引起的慢性炎癥狀態(tài)。以脂多糖或卡介苗作為致炎物質(zhì)的氣囊炎癥模型達(dá)到慢性炎癥狀態(tài)的時間為造模后1周左右。 2.不同慢性炎癥-黑色素瘤復(fù)合模型的效果：在所有小鼠的肺、肝、脾中均未發(fā)現(xiàn)黑色素瘤轉(zhuǎn)移灶；A組腫瘤重量在建模后第21d差異有統(tǒng)計學(xué)意義,B組腫瘤重量在建模后第15d、21d差異有統(tǒng)計學(xué)意義,體積在第21d有統(tǒng)計學(xué)差異。C組腫瘤和D組腫瘤和體積重量在建模后第9d、21d差異有統(tǒng)計學(xué)意義。 3.不同慢性炎癥-黑色素瘤復(fù)合模型中MVD的表現(xiàn)：A組腫瘤MVD在建模后第9d、15d、21d、27d差異有統(tǒng)計學(xué)意義。B組腫瘤MVD在建模后第9d、15d、21d、27d差異有統(tǒng)計學(xué)意義。C組腫瘤MVD在建模后第21d差異有統(tǒng)計學(xué)意義。D組腫瘤建模后第15d、21d差異有統(tǒng)計學(xué)意義。 結(jié)論： 1.建立了小鼠皮下氣囊慢性炎癥模型,此模型能較好地模擬在單一致炎因子存在情況下引起的慢性炎癥狀態(tài)。 2.建立四種對腫瘤生長有影響的動物模型,在致炎物質(zhì)選取,致炎部位處理,各有不同。氣囊弗氏佐劑組中慢性炎癥對腫瘤生長產(chǎn)生影響效果最為顯著,認(rèn)為氣囊弗氏佐劑組(B組)優(yōu)于其它組。 3.觀察模型發(fā)現(xiàn),慢性炎癥可以促進(jìn)腫瘤體積、重量的增長。 4.通過對腫瘤MVD的觀察,發(fā)現(xiàn)慢性炎癥能促進(jìn)遠(yuǎn)位腫瘤血管的生長。
[Abstract]:Objective:
To explore the method of establishing a compound model of chronic inflammation and melanoma in mice, by observing the effect of chronic inflammation on tumor volume, weight, and microvascular density, the effect of chronic inflammation on the growth of transplanted tumor is clarified, and the experimental basis for further study of the effect of inflammatory factors on tumor growth is laid.
Content:
1. the establishment of a chronic inflammatory model: injection of air into the back of the mouse to make air sac, injecting the inflammatory lipopolysaccharide into the air bag, BCG, observing the effect of the model of chronic inflammation and the chronic period of inflammation.
2. establishment of melanoma model: after 75% alcohol cotton balls were sterilized in mice, melanoma cell suspensions were injected subcutaneously into the groin.
3. the establishment of a compound model of chronic inflammatory melanoma: injection of air to make air sac or embedded glass beads in the back of mice, injecting substances into the air bag or around the glass beads every week. 3 days after the initial injection of inflammatory substances, four kinds of chronic inflammation melanoma in mice were injected subcutaneously into the groin. The composite model is more suitable for chronic inflammation and melanoma.
4. the effect of chronic inflammation on the volume, weight, and distant metastasis of melanoma: a compound model of chronic inflammatory melanoma was established to compare the volume of tumor, weight, and the rate of metastasis of lung, liver and spleen in the control group and the experimental group.
5. the effect of chronic inflammation on microvascular density: HE staining was used to compare the microvascular density between the control group and the experimental group, and the changes of endothelium dependent blood vessels in the inflammatory environment were determined.
Method錛，

本文編號：2014773