本文選題：皮膚惡性腫瘤 + 骨橋蛋白　； 參考：《吉林大學》2012年碩士論文

【摘要】：目的:通過免疫組化技術(shù)檢測OPN與MMP-2在惡性黑素瘤、基底細胞癌及色素痣中的表達規(guī)律，探討OPN與MMP-2在皮膚惡性腫瘤向外侵襲過程中的作用。 方法:采集臨床標本44例，分為惡性黑素瘤組(n=16)，基底細胞癌組(n=14)，色素痣組(n=14)。應(yīng)用免疫組化法(SP法)檢測各標本切片中OPN與MMP-2的表達。觀察每組標本中陽性細胞的數(shù)量、染色程度，分析OPN與MMP-2在三組中表達的水平差異和相關(guān)性。分析數(shù)據(jù)用SPSS l8.0統(tǒng)計軟件進行統(tǒng)計學分析。 結(jié)果:①惡性黑素瘤中OPN的表達率為93.8%。在基底細胞癌中OPN陽性表達率為71.4%。色素痣組中OPN陽性表達率為14.3%。OPN在惡性黑素瘤組與色素痣組相比有明顯顯著性差異(P0.01)�；准毎┙M與色素痣組相比有明顯顯著性差異(P0.01)。惡性黑素瘤組與基底細胞癌組相比無顯著差異性（P＞0.05）。②MMP-2在惡性黑素瘤中的陽性表達率為87.5%，，在基底細胞癌中陽性表達率為64.3%，在色素痣組中陽性表達率為7.1%。MMP-2在惡性黑素瘤組與色素痣組相比有明顯顯著性差異(P0.01)�；准毎┙M與色素痣組相比有顯著性差異(P0.01)。惡性黑素瘤組與基底細胞癌組相比無顯著差異性（P＞0.05）。③OPN與MMP-2在惡性黑素瘤、基底細胞癌中的表達具有相關(guān)性(P0.05)。 結(jié)論： ①在皮膚惡性腫瘤中，OPN和MMP-2表達呈正相關(guān)。 ②MMP-2在惡性黑素瘤中高表達。 ③OPN、MMP-2在基底細胞癌中高表達。
[Abstract]:Objective: to investigate the expression of OPN and MMP-2 in malignant melanoma, basal cell carcinoma and nevus pigmentosa by immunohistochemical technique, and to explore the role of OPN and MMP-2 in the outward invasion of skin malignant tumor. Methods: 44 clinical specimens were collected and divided into malignant melanoma group (n = 16), basal cell carcinoma group (n = 14) and pigmented nevus group (n = 14). The expression of OPN and MMP-2 was detected by immunohistochemical staining (SP method). The number and staining degree of positive cells in each group were observed, and the difference and correlation of OPN and MMP-2 expression in the three groups were analyzed. The data were analyzed by SPSS 1. 0. 0 statistical software. Results the expression rate of OPN in malignant melanoma was 93.8%. The positive expression rate of OPN was 71.4 in basal cell carcinoma. The positive expression rate of OPN in nevus group was significantly higher than that in nevus group (P 0.01). The positive expression rate of 14.3%.OPN in melanoma group was significantly higher than that in nevus pigmented nevus group. There was significant difference between basal cell carcinoma group and pigmented nevus group. There was no significant difference between malignant melanoma group and basal cell carcinoma group. The positive expression rate of P > 0.05).2MMP-2 was 87.5 in malignant melanoma, 64.3 in basal cell carcinoma and 7.1%.MMP-2 in pigmented nevus. Compared with nevus pigmentosa group, there was a significant difference in tumor group and nevus group (P 0.01). There was significant difference between basal cell carcinoma group and pigmented nevus group. There was no significant difference between malignant melanoma group and basal cell carcinoma group (P > P > 0.05).3OPN and MMP-2) expression in malignant melanoma was correlated with that in basal cell carcinoma (BCC). Conclusion: There was a positive correlation between the expression of OPN and MMP-2 in malignant skin tumors. 2MMP-2 is highly expressed in malignant melanoma. 3The expression of MMP-2 in basal cell carcinoma was high.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R739.5

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