本文選題：甘草酸 + 特應(yīng)性皮炎　； 參考：《重慶醫(yī)科大學(xué)學(xué)報》2017年12期

【摘要】：目的:探討甘草酸對實驗性特應(yīng)性皮炎(atopic dermatitis,AD)炎癥的抑制作用及可能機(jī)制。方法:用2,4-二硝基氯苯(2,4-dinitrochlorobenzene,DNCB)在6~8周齡雌性BALB/c小鼠建立實驗性特應(yīng)性皮炎模型。將小鼠分為4組:對照組,甘草酸治療組(分別50 mg/kg、20 mg/kg和10 mg/kg)每天腹腔注射。分別在第8、15天,HE染色觀察皮膚病理學(xué)改變,用qRT-PCR方法測定局部皮損組織Th2型細(xì)胞因子的mRNA水平。同時,在第15天,取小鼠脾淋巴細(xì)胞體外培養(yǎng),刀豆蛋白刺激24 h后,ELISA檢測Th2型細(xì)胞因子的表達(dá)。結(jié)果:在第8天,與對照組相比,甘草酸各治療組的皮膚病理學(xué)改變、局部皮損中細(xì)胞因子的mRNA水平以及脾淋巴細(xì)胞分泌的細(xì)胞因子無統(tǒng)計學(xué)差異。但在第15天,與對照組表皮厚度(194.00±18.43)和真皮厚度(374.70±40.01)比較,甘草酸50 mg/(kg·d)治療組小鼠表皮厚度(141.00±7.61)(P=0.005)和真皮厚度(281.20±69.42)(P=0.012)顯著降低。qRT-PCR結(jié)果顯示皮損中白介素(inteleukin,IL)-4 mRNA(0.036±0.011)和IL-13 mRNA(0.051±0.022)水平分別低于對照組(0.073±0.007,0.100±0.015)(P=0.000,P=0.001)。ELISA方法檢測脾淋巴細(xì)胞分泌的IL-4(42.94±9.26)和IL-13(43.34±12.59)均明顯低于對照組(61.87±10.20,77.42±10.71)(P=0.002,P=0.000)。結(jié)論:甘草酸能緩解小鼠特應(yīng)性皮炎皮膚炎癥,可能是通過下調(diào)Th2細(xì)胞因子水平發(fā)揮作用。
[Abstract]:Aim: to investigate the inhibitory effect of glycyrrhizic acid (glycyrrhizic acid) on the inflammation of experimental atopic dermatitis (AD) and its possible mechanism. Methods: the experimental atopic dermatitis model was established in 6-week-old female BALB/c mice by using 2O4-dinitrochlorobenzeneCBB (DNCBB). Mice were divided into four groups: control group, glycyrrhizic acid treatment group (50 mg / kg / kg 20 mg/kg and 10 mg / kg) daily intraperitoneal injection. The pathological changes of skin were observed by HE staining on the 8th day and the mRNA levels of Th2 type cytokines in local lesions were measured by qRT-PCR method. At the same time, the spleen lymphocytes of mice were cultured in vitro on the 15th day, and the expression of Th2 type cytokines was detected by Elisa after 24 h stimulation with concanavalin. Results: on the 8th day, there was no significant difference in the pathological changes of skin, the level of cytokines mRNA in local skin lesions and the cytokines secreted by spleen lymphocytes in each treatment group compared with the control group. But on the 15th day, the thickness of epidermis was 194.00 鹵18.43) and the thickness of dermis was 374.70 鹵40.01). 鐢樿崏閰，

本文編號：1951599