本文選題：皮膚鱗癌 + 食管鱗癌�。� 參考：《石河子大學(xué)》2011年碩士論文

【摘要】：目的：確定皮膚鱗癌和食管鱗癌中感染HHV-8的陽性率,比較兩種鱗癌間感染HHV-8的差異性,確定感染HHV-8 K1基因亞型及K15等位基因型。并初步探討感染人類皰疹病毒8型K1、K15與鱗狀細(xì)胞癌的發(fā)病關(guān)系。 方法：對(duì)40例皮膚鱗癌、40例食管鱗癌石蠟包埋組織使用序列特異性引物-巢式PCR技術(shù)(NestedPCR)對(duì)人類皰疹病毒8型的DNA抽提,對(duì)K1和K15基因的部分片段進(jìn)行擴(kuò)增、雙向測(cè)序,使用DNASTAR軟件、Crustal W軟件和PHYLIP軟件包對(duì)測(cè)序結(jié)果進(jìn)行系統(tǒng)發(fā)生學(xué)分析,從而確定HHV-8K1和K15基因亞型,最后運(yùn)用χ2檢驗(yàn)和Fisher確切概率法對(duì)結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果：(K1檢測(cè)結(jié)果)：①40例皮膚鱗癌9例(22.5%)感染HHV-8 K1,40例食管鱗癌8例(20%)感染HHV-8 K1,HHV-8在皮膚鱗癌與食管鱗癌感染率組間比較差異無統(tǒng)計(jì)學(xué)意義(P0.05),即80例鱗癌中有17例感染HHV-8,這17例病毒株中,14例是K1-C亞型占82.35%(均為C2),3例是K1-A亞型(2例為A3,1例為A1)；17例均為K15-P等位基因型占100%；14例為C/P嵌合型(其中14例為C2/P嵌合型)占82.35%。新疆鱗癌感染的HHV-8主要屬于K1-C亞型和K15-P等位基因型,以C2/P嵌合型為主,同時(shí)也存在部分的K1-A亞型感染者。②皮膚鱗癌感染HHV-8 K1亞型1例A亞型,8例C亞型,食管鱗癌感染HHV-8 K1亞型2例A亞型,5例C亞型,未發(fā)現(xiàn)其他亞型。(K15檢測(cè)結(jié)果)①9例皮膚鱗癌(22.5%)石蠟包埋組織中檢測(cè)為HHV-8 K15P型,8例食管鱗癌(20%)石蠟包埋組織中檢測(cè)到HHV-8K15P型,兩種腫瘤均未檢測(cè)出K15M基因型；②HHV-8在皮膚鱗癌與食管鱗癌感染率組間比較差異無統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論：①新疆鱗癌與新疆不同民族自然人群血清中HHV-8檢出率相近,提示HHV-8在鱗狀細(xì)胞癌中的感染或許一種隨機(jī)感染,與鱗狀細(xì)胞癌的發(fā)病并沒有直接的聯(lián)系。②感染皮膚鱗癌與食管鱗癌感染HHV-8在組間比較差異無統(tǒng)計(jì)學(xué)意義。③鱗狀細(xì)胞癌中感染的HHV-8亞型主要為K1-C亞型和K15 P等位基因型,存在部分的K1-A亞型。
[Abstract]:Objective: to determine the positive rate of HHV-8 infection in squamous cell carcinoma of skin and esophagus, to compare the difference of HHV-8 infection between the two types of squamous cell carcinoma, and to determine the subtype of HHV-8 K1 gene and the genotype of K15 allele. To investigate the relationship between human herpesvirus 8 K1 K15 infection and squamous cell carcinoma (SCC). Methods: DNA of human herpesvirus 8 was extracted from 40 cases of squamous cell carcinoma of skin and 40 cases of esophageal squamous cell carcinoma with paraffin embedded tissue using sequence specific primer nested PCR technique. The partial fragments of K1 and K15 genes were amplified and sequenced. DNASTAR software Crustal W software and PHYLIP software package were used to analyze the sequence results, and the subtypes of HHV-8K1 and K15 genes were determined. Finally, the results were statistically analyzed by 蠂 2 test and Fisher exact probability method. Results there was no significant difference in the infection rate of HHV-8 K1 and esophageal squamous cell carcinoma between HHV-8 K1 and esophageal squamous cell carcinoma group (P 0.05), that is, 17 out of 80 cases of squamous cell carcinoma (17 cases of squamous cell carcinoma of the esophagus) were infected with HHV-8 K1 and HHV-8 (P < 0.05). The results showed that there was no significant difference between the infection rate of HHV-8 K1 and esophageal squamous cell carcinoma (P 0.05), that is, 17 out of 80 cases of squamous cell carcinoma of the esophagus (17 cases of squamous carcinoma of the esophagus) Of the 17 cases infected with HHV-8, 14 cases were K1-C subtype (3 cases were K1-A subtype), 2 cases were A3, 1 case was A3, 17 cases were K15-P allele, 14 cases were C- P chimeric type (14 cases were C2P chimeric type), accounting for 82.35%. The HHV-8 of squamous cell carcinoma infection in Xinjiang mainly belonged to K1-C subtype and K15-P allele, and C2 / P chimeric type was the main genotype. There were also some K1-A subtype infected with HHV-8 K1 subtype A subtype and 8 cases with C subtype of HHV-8 K1 subtype. HHV-8 K1 subtype A subtype was detected in 2 cases of esophageal squamous cell carcinoma infection. No other subtypes were found. K15 was detected in 19 cases of squamous cell carcinoma of skin (22. 5%) HHV-8K15P type was detected in paraffin-embedded tissues of 8 cases of HHV-8 K15P type and 8 cases of esophageal squamous cell carcinoma. There was no significant difference of K15M genotype 2HHV-8 in the infection rate of cutaneous squamous cell carcinoma and esophageal squamous cell carcinoma between the two groups (P 0.05). Conclusion the detection rate of HHV-8 in serum of Xinjiang squamous cell carcinoma is similar to that of different ethnic groups in Xinjiang, suggesting that the infection of HHV-8 in squamous cell carcinoma may be a random infection. There was no direct relationship between squamous cell carcinoma (SCC) and squamous cell carcinoma (SCC). 2 there was no significant difference between the infected SCC and esophageal squamous cell carcinoma (HHV-8) infection. The HHV-8 subtypes of SCC infection were mainly K1-C subtype and K15 P allele. There are partial K1-A subtypes.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R735.1;R739.5

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