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  本文選題：△Np63 + 皮膚良性增殖性疾病��； 參考：《昆明醫(yī)科大學(xué)》2012年碩士論文

【摘要】：實(shí)驗(yàn)?zāi)康模?1.研究△Np63在正常皮膚、尋常型銀屑病、脂溢性角化癥、日光性角化病、基底細(xì)胞癌組織中的表達(dá)及意義。 2.探討△Np63在皮膚良、惡性增殖性疾病中的診斷及鑒別診斷價值。 實(shí)驗(yàn)方法： 收集、整理近兩年昆明醫(yī)科大學(xué)第一附屬醫(yī)院皮膚科病理室的、符合診斷標(biāo)準(zhǔn)的尋常型銀屑病(PS)、脂溢性角化癥(SK)、日光性角化病(AK)、基底細(xì)胞癌(BCC)各30例,并選取皮膚科手術(shù)室及頜面外科手術(shù)切除的正常皮膚(Normal Skin)10例。經(jīng)過脫水、包埋、切片等步驟,采用免疫組化技術(shù)SP法對切片進(jìn)行△Np63染色,然后在光學(xué)顯微鏡下觀察切片,每張切片在皮膚病變組織中隨機(jī)選取5個不連續(xù)的視野,用OLYMPUS BX51顯微圖像采集系統(tǒng)拍攝圖片,應(yīng)用Image-Pro Plus6.0(IPP)圖像分析軟件定量測定每個視野區(qū)域內(nèi)陽性表達(dá)的平均光密度值(density mean),最后通過spss17.0軟件進(jìn)行統(tǒng)計、分析病種之間的差異。陰性對照：PBS代替一抗,結(jié)果陰性。 實(shí)驗(yàn)結(jié)果： 1.△Np63在10例正常皮膚、30例尋常型銀屑病、30例脂溢性角化癥、30例日光性角化病、30例基底細(xì)胞癌中均有陽性表達(dá)。正常皮膚組,△Np63主要表達(dá)在表皮基底層細(xì)胞及近基底層細(xì)胞的細(xì)胞核中；尋常型銀屑病及日光性角化病組,ANp63主要表達(dá)在表皮基底層及棘層中下1/3細(xì)胞的細(xì)胞核中;脂溢性角化癥組,△Np63主要表達(dá)于表皮基底層及棘層細(xì)胞的細(xì)胞核中；基底細(xì)胞癌組,ANp63主要表達(dá)于基底樣細(xì)胞組成的團(tuán)塊中呈彌漫的陽性表達(dá)。 2.經(jīng)IPP軟件統(tǒng)計,△Np63在各病種中的表達(dá)依次為：正常皮膚組的平均光密度值為0.0056±0.0019；日光性角化病組的平均光密度值為0.0262±0.0184；尋常型銀屑病組的平均光密度值為0.0266±0.0100；脂溢性角化癥組的平均光密度值為0.0391±0.0169；基底細(xì)胞癌組的平均光密度值為0.0498±0.0222。 3.經(jīng)統(tǒng)計學(xué)分析,△Np63在各病種之間平均光密度的表達(dá)不同或不全相同(P0.001),差異有統(tǒng)計學(xué)意義；進(jìn)一步進(jìn)行兩兩比較,除日光性角化病及尋常型銀屑病之間(P0.05)差異無統(tǒng)計學(xué)意義以外,其余各組之間兩兩比較(P0.05),差異均有統(tǒng)計學(xué)意義。其中△Np63在基底細(xì)胞癌組的表達(dá)最強(qiáng),其次分別為脂溢性角化癥組、尋常型銀屑病組、日光性角化病組和正常皮膚組。 4.△Np63在各病種中的表達(dá)與患者的性別、年齡、發(fā)病部位(曝光部位和非曝光部位)均無統(tǒng)計學(xué)意義(P0.05)。 實(shí)驗(yàn)結(jié)論： 1.△Np63與復(fù)層鱗狀上皮基底細(xì)胞的發(fā)育和增殖密切相關(guān)。 2.△Np63可作為是未分化上皮來源腫瘤的標(biāo)記物。 3.△Np63可以考慮作為脂溢性角化癥及日光性角化病鑒別診斷的免疫學(xué)指標(biāo)。 4.△Np63蛋白的表達(dá)與患者的性別、年齡、日曬無相關(guān)性。
[Abstract]:Objective: 1. To study the expression and significance of Np63 in normal skin, psoriasis vulgaris, seborrheic keratosis, sunlight keratosis and basal cell carcinoma. 2. To investigate the value of Np63 in the diagnosis and differential diagnosis of benign and malignant proliferative diseases of skin. Experimental methods: In the past two years, 30 cases of psoriasis vulgaris, SKK, AKK, BCCs and BCCs were collected from the dermatology department of the first affiliated Hospital of Kunming Medical University, which met the diagnostic criteria. Normal Skin)10 cases of normal skin were selected from dermatological surgery room and maxillofacial surgery. After dehydration, embedding and slicing, immunohistochemical SP method was used to stain the sections with Np63, and then the sections were observed under optical microscope. Five discontinuous visual fields were randomly selected from each slice in the skin lesion tissue. OLYMPUS BX51 microscopic image acquisition system was used to take pictures, and Image-Pro plus 6.0 / IP) image analysis software was used to quantitatively measure the mean optical density of positive expression in each visual field. Finally, the difference of disease species was analyzed by spss17.0 software. The negative control group: PBS replaced the first antibody and the result was negative. Experimental results: 1. Np63 positive expression was found in 30 cases of seborrheic keratosis and 30 cases of basal cell carcinoma in 10 cases of normal skin and 30 cases of psoriasis vulgaris. In normal skin group, Np63 was mainly expressed in the nuclei of epidermal basal cells and proximal basal cells, while in psoriasis vulgaris and sunburn keratosis, ANp63 was mainly expressed in the nuclei of 1 / 3 cells in epidermal basal layer and lower layer of spinous layer. In seborrheic keratosis group, Np63 was mainly expressed in the nucleus of basal layer and spinous layer of epidermis, and ANp63 was expressed diffusely in the mass composed of basal cell in basal cell carcinoma group. 2. According to IPP software statistics, The average optical density of Np63 in normal skin group was 0.0056 鹵0.0019, that of solar keratosis group was 0.0262 鹵0.01844, that of psoriasis vulgaris group was 0.0266 鹵0.0100, and that of seborrheic keratosis group was 0.0266 鹵0.0100. The optical density was 0.0391 鹵0.0169 and the average optical density in basal cell carcinoma was 0.0498 鹵0.0222. 3. By statistical analysis, the average optical density of Np63 was different or not the same among the disease species, and the difference was statistically significant. There was no significant difference in P0.05 between solar keratosis and psoriasis vulgaris, but there was significant difference between the other two groups. The expression of Np63 was strongest in basal cell carcinoma group, followed by seborrheic keratosis group, psoriasis vulgaris group, sunlight keratosis group and normal skin group. 4. There was no significant difference between the expression of Np63 and the sex, age, location of the disease (exposure and non-exposure) of the patients (P 0.05). The experimental conclusions are as follows: 1. Np63 is closely related to the development and proliferation of basal cells in lamellar squamous epithelium. 2. Np63 can be used as a marker of undifferentiated epithelial-derived tumors. 3. Np63 may be considered as an immunological index for differential diagnosis of seborrheic keratosis and solar keratosis. 4. There was no correlation between the expression of Np63 protein and sex, age and sun exposure.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R739.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 高天文,孫東杰,李春英,何弘,李青,劉友生,刁慶春,黃高升,郝飛,鐘白玉,馬福成,柳鳳軒,閆小初,劉東梅,劉玉峰,劉榮卿;中國西部兩醫(yī)院1905例皮膚惡性腫瘤回顧分析[J];北京大學(xué)學(xué)報(醫(yī)學(xué)版);2004年05期

2 廖文俊,高天文;表皮干細(xì)胞的研究進(jìn)展[J];國外醫(yī)學(xué)(皮膚性病學(xué)分冊);2003年04期

3 鄭博文;鄒勇莉;何黎;杜紅霞;付蘭;;皮膚惡性腫瘤568例回顧性分析[J];中國皮膚性病學(xué)雜志;2009年12期

，

本文編號：1849509