發(fā)布時(shí)間：2018-04-14 06:11

  本文選題：基因沉默 + 惡性黑素瘤A細(xì)胞　； 參考：《臨床皮膚科雜志》2017年01期

【摘要】：目的:研究microRNA-192基因表達(dá)降低與惡性黑素瘤A375細(xì)胞增殖及凋亡的關(guān)系。方法:除空白對(duì)照組外,運(yùn)用脂質(zhì)體介導(dǎo)法將空載體和為microRNA-192設(shè)計(jì)的small interfering RNA(si RNA)轉(zhuǎn)染到惡性黑素瘤A375細(xì)胞中,并利用實(shí)時(shí)定量PCR檢測(cè)microRNA-192的表達(dá)。通過(guò)形態(tài)學(xué)觀察、細(xì)胞計(jì)數(shù)法、3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽(MTT)比色法以及流式細(xì)胞免疫學(xué)等方法,分析空白對(duì)照組、空載體對(duì)照組(lipofectamine2000)和實(shí)驗(yàn)組惡性黑素瘤A375細(xì)胞的增殖和早期凋亡情況。結(jié)果:空白對(duì)照組和空載體對(duì)照組之間相比較,細(xì)胞增殖和凋亡情況幾乎一致,差異無(wú)統(tǒng)計(jì)學(xué)意義。與空載體對(duì)照組相比,實(shí)驗(yàn)組存活的惡性黑素瘤A375細(xì)胞的活性和數(shù)量明顯降低,此現(xiàn)象并隨著si RNA濃度的提高而愈發(fā)明顯。另外,實(shí)驗(yàn)組惡性黑素瘤A375細(xì)胞細(xì)胞凋亡指數(shù)較空載體對(duì)照組明顯升高。結(jié)論:通過(guò)下調(diào)惡性黑素瘤細(xì)胞中microRNA-192基因表達(dá)可能會(huì)有效地抑制惡性黑素瘤的生長(zhǎng)并促使其細(xì)胞凋亡。
[Abstract]:Aim: to study the relationship between the decrease of microRNA-192 gene expression and the proliferation and apoptosis of malignant melanoma A375 cells.Methods: in addition to the blank control group, the empty vector and small interfering RNA(si designed for microRNA-192 were transfected into malignant melanoma A375 cells by liposome mediated transfection, and the expression of microRNA-192 was detected by real-time quantitative PCR.By means of morphological observation, cell counting method and MTT colorimetric method, the blank control group was analyzed, and the results were as follows: (1) the cell count method was 3 ~ (4) C ~ (- 4) -dimethylthiazole-5-dimethylthiazolium-(2) -diphenyl tetrazolium bromide (MTT) colorimetry and flow cytometry.The proliferation and early apoptosis of A375 cells in the empty vector control group and in the experimental group.Results: the cell proliferation and apoptosis were almost the same between the blank control group and the empty carrier control group, but the difference was not statistically significant.Compared with the empty vector control group, the activity and quantity of A375 cells in the experimental group were significantly decreased, and this phenomenon became more obvious with the increase of si RNA concentration.In addition, the apoptotic index of malignant melanoma A375 cells in the experimental group was significantly higher than that in the empty vector control group.Conclusion: down-regulation of microRNA-192 gene expression in malignant melanoma cells may effectively inhibit the growth and induce apoptosis of malignant melanoma cells.
【作者單位】： 鄭州大學(xué)第二附屬醫(yī)院皮膚科;河北醫(yī)科大學(xué)附屬第四醫(yī)院皮膚科;
【分類(lèi)號(hào)】：R739.5
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本文編號(hào)：1748047