本文選題：銀屑病 + 流式細(xì)胞術(shù)��； 參考：《中國皮膚性病學(xué)雜志》2014年01期

【摘要】：目的研究阿維A調(diào)控銀屑病患者外周血樹突狀細(xì)胞功能,探討阿維A治療銀屑病作用機(jī)制。方法抽取銀屑病患者外周血,分離單個核細(xì)胞,體外誘導(dǎo)培養(yǎng)樹交狀細(xì)胞(Dendritic cells,DCs)。不同濃度阿維A(Inmol/L和10nmol/L)作用DCs 24h,對照組為只加培養(yǎng)基,流式細(xì)胞術(shù)(Flow cytometry,FCM)檢測DCs中CD80,CD83及CD86表達(dá)水平,酶聯(lián)免疫吸附試驗(ELISA)方法檢測細(xì)胞培養(yǎng)上清液中IL-12表達(dá)水平。結(jié)果體外成功培養(yǎng)出DCs,流式細(xì)胞術(shù)檢測到1,10nmol/L阿維A作用DCs24h后,CD80,CD83及CD86表達(dá)率分別為(69.74±3.83)%、(61.12±3.03)%、(70.18±3.32)%和(60.19±3.97)%、(50.78±4.81)%、(48.96±3.18)%,顯著低于對照組DCs上CD80,CD83及CD86表達(dá)率(75.15±2.76)%、(72.43±1.94)%、(77.81±2.46)%(P0.01)。1,10nmol/L阿維A作用DCs 24h后,DCs分泌到細(xì)胞培養(yǎng)液中的IL-12表達(dá)水平分別為(260.36±15.26)(226.98±17.24)顯著低于對照組(282.80±23.26)(P0.01)。結(jié)論阿維A可以降低銀屑病患者外周血中DCs上CD80.CD83及CD86表達(dá)率,抑制DCs活化,且降低IL-12因子的分泌量,能夠調(diào)控患者機(jī)體的免疫功能,從而對銀屑病起到治療作用。
[Abstract]:Objective to study the regulation of Avera on the function of peripheral blood dendritic cells in patients with psoriasis and to explore the mechanism of Avera in the treatment of psoriasis.Methods Mononuclear cells were isolated from peripheral blood of patients with psoriasis, and dendritic cells were cultured in vitro.DCs was treated with Avi A(Inmol/L and 10nmol / L for 24 h. Flow cytometry was used to detect the expression of CD80 CD83 and CD86 in DCs. Elisa was used to detect the expression of IL-12 in the supernatant of cell culture. The expression of CD80 CD83 and CD86 in the supernatant of cell culture was detected by flow cytometry (FCM) and enzyme linked immunosorbent assay (Elisa).緇撴灉浣撳鎴愬姛鍩瑰吇鍑篋Cs,嫻佸紡緇嗚優(yōu)鏈嫻嬪埌1,10nmol/L闃跨淮A浣滅敤DCs24h鍚，

本文編號：1742368