本文選題：尋常型銀屑病　切入點(diǎn)：HBD-2　出處：《山西醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的：探討HBD-2、IL-lβ和NF-κBp65在尋常型銀屑病皮損中的表達(dá)與尋常型銀屑病嚴(yán)重程度的關(guān)系及其在尋常型銀屑病中的作用機(jī)制。 方法：1.采用免疫組化法檢測(cè)45例尋常型銀屑病患者皮損組織、45例尋常型銀屑病患者非皮損組織及15例正常皮膚組織標(biāo)本中HBD-2、IL-lβ、NF-κBp65的表達(dá),比較不同組別間三者表達(dá)的差異,并對(duì)HBD-2、IL-lβ、NF-κBp65進(jìn)行相關(guān)性分析。2.根據(jù)PASI評(píng)分將45例尋常型銀屑病患者分為三組：輕度組25例、中度組12例、重度組8例。檢測(cè)輕、中、重尋常型銀屑病患者皮損區(qū)HBD-2、IL-lβ、NF-κBp65的表達(dá),比較不同組別間三者表達(dá)的差異,分析三者在尋常型銀屑病患者皮損中的相關(guān)性。 結(jié)果：1.尋常型銀屑病患者皮損區(qū)HBD-2、IL-lβ、NF-κBp65的表達(dá)比尋常型銀屑病患者非皮損區(qū)、正常人皮膚明顯上調(diào),在尋常型銀屑病患者非皮損區(qū)HBD-2、IL-lβ的表達(dá)亦高于正常人皮膚,差異均有統(tǒng)計(jì)學(xué)意義(均P0.05)。2. HBD-2、IL-lβ、NF-κBp65在尋常型銀屑病重度患者皮損區(qū)的表達(dá)明顯高于中度組、輕度組、正常對(duì)照組；其在中度患者皮損區(qū)的表達(dá)高于輕度組及正常對(duì)照組,并且輕度組高于正常對(duì)照組,差異均具有統(tǒng)計(jì)學(xué)意義(均P0.05)。3.在輕、中、重尋常型銀屑病患者皮損中HBD-2與lL-lβ, HBD-2與NF-KBp65、IL-lβ與NF-κBp65表達(dá)水平之間均存在正相關(guān)(r=0.869、0.740、0.814,均P0.05)。 結(jié)論：1. HBD-2、IL-lβ在正常人皮膚組織、尋常型銀屑病非皮損區(qū)、尋常型銀屑病皮損區(qū)中表達(dá)呈逐漸上調(diào),提示HBD-2、IL-lβ與尋常型銀屑病的發(fā)生、發(fā)展過程密切相關(guān)。2.NF-κB p65在尋常型銀屑病皮損區(qū)表達(dá)上調(diào),提示NF-κB p65與尋常型銀屑病發(fā)病密切相關(guān)。3.本實(shí)驗(yàn)結(jié)果表明HBD-2、IL-lβ、NF-κB p65在尋常型銀屑病皮損中的表達(dá)均升高,且三者之間均存在正相關(guān),我們推測(cè)三者之間可能存在相互作用共同參與了銀屑病的發(fā)病過程。
[Abstract]:Objective: to investigate the relationship between the expression of IL-l 尾 and NF- 魏 Bp65 in psoriasis vulgaris and the severity of psoriasis vulgaris.Method 1: 1.The expression of HBD-2mIL-l 尾 -NF- 魏 Bp65 in 45 cases of psoriasis vulgaris and 15 cases of normal skin tissue was detected by immunohistochemical method, and the differences among the three groups were compared.The correlation between IL-1 尾 and NF- 魏 Bp65 of HBD-2 was analyzed.According to PASI score, 45 patients with psoriasis vulgaris were divided into three groups: mild group (25 cases), moderate group (12 cases) and severe group (8 cases).To detect the expression of IL-l 尾 -NF- 魏 Bp65 in lesions of patients with mild, moderate and severe psoriasis, to compare the differences among the three groups, and to analyze the correlation among them in the lesions of patients with psoriasis vulgaris.The result is 1: 1.The expression of IL-l 尾 -NF- 魏 Bp65 in the lesions of psoriasis vulgaris was significantly higher than that in the non-lesions of psoriasis vulgaris and in the normal skin, and the expression of IL-l 尾 in the non-lesions of psoriasis vulgaris was significantly higher than that in the normal skin (all P 0.05. 2).The expression of IL-l 尾 -NF- 魏 Bp65 in severe psoriasis vulgaris was significantly higher than that in moderate group, mild group and normal control group, and was higher in moderate group than in mild group and normal control group, and in mild group was higher than that in normal control group.The difference was statistically significant (all P 0.05. 3).There was a positive correlation between the expressions of HBD-2 and lL-l 尾, HBD-2 and NF-KBp65, IL-l 尾 and NF- 魏 Bp65 in patients with mild, moderate and severe psoriasis vulgaris (P 0.05).Conclusion 1.The expression of IL-l 尾 in normal human skin, psoriasis vulgaris and psoriasis vulgaris was gradually up-regulated, suggesting the occurrence of IL-l 尾 and psoriasis vulgaris.2. The expression of NF- 魏 B p65 in psoriasis vulgaris was up-regulated, suggesting that NF- 魏 B p65 was closely related to the pathogenesis of psoriasis vulgaris.The results showed that the expression of IL-l 尾 -NF- 魏 B p65 in psoriasis vulgaris was increased, and there was a positive correlation among them. We speculate that the interaction among them may be involved in the pathogenesis of psoriasis.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R758.63

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本文編號(hào)：1705473