本文選題：P63基因　切入點(diǎn)：鮑溫樣丘疹病　出處：《昆明醫(yī)學(xué)院》2011年碩士論文

【摘要】：實(shí)驗(yàn)?zāi)康模?1、探討ΔNp63在正常皮膚、鮑溫樣丘疹病、鮑溫病、皮膚鱗狀細(xì)胞癌組織中的表達(dá)及意義。 2、探討鮑溫樣丘疹病與鮑溫病的免疫學(xué)鑒別診斷指標(biāo)。 實(shí)驗(yàn)方法： 用免疫組化Envision法檢測(cè)ΔNp63的表達(dá),其中正常皮膚10例、鮑溫樣丘疹病30例、鮑溫病30例、皮膚鱗狀細(xì)胞癌30例。PBS代替—抗,作為陰性對(duì)照。 實(shí)驗(yàn)結(jié)果： 1、ΔNp63在10例正常皮膚組織的表皮基底層及表皮下1/3的細(xì)胞表達(dá),在30例鮑溫樣丘疹病組織中6例為表皮內(nèi)彌漫表達(dá),24例為表皮基底層及表皮下1/3的細(xì)胞表達(dá)；在30例鮑溫病組織中1例陰性表達(dá),29例為表皮內(nèi)彌漫表達(dá)；在30例皮膚鱗狀細(xì)胞癌組織的表皮及真皮內(nèi)癌巢的基底樣細(xì)胞表達(dá),在高分化鱗癌(21例)的癌巢外周呈環(huán)狀表達(dá),在中分化鱗癌(9例)的癌巢中有較彌漫的表達(dá)。 2、SPSS13.0統(tǒng)計(jì)軟件進(jìn)行X2檢驗(yàn),ΔNp63在鮑溫病、皮膚鱗狀細(xì)胞癌組中的表達(dá)相比較差異有統(tǒng)計(jì)學(xué)意義(X2=0.001,P0.05),在鮑溫病組中的陽(yáng)性表達(dá)率高于皮膚鱗狀細(xì)胞癌組。ΔNp63在鮑溫樣丘疹病組和鮑溫病組中表達(dá)的比較差異有統(tǒng)計(jì)學(xué)意義(X2=0.000,P0.05),鮑溫病組中的陽(yáng)性表達(dá)率高于鮑溫樣丘疹病組。ΔNp63在皮膚鱗狀細(xì)胞癌組中與鮑溫樣丘疹病組中表達(dá)的比較(X2=0.125,P0.05),無(wú)統(tǒng)計(jì)學(xué)差異。ΔNp63在鮑溫樣丘疹病組與正常皮膚組中表達(dá)的比較(X2=0.072,P0.05)無(wú)統(tǒng)計(jì)學(xué)差異。正常皮膚組和皮膚鱗狀細(xì)胞癌組中ΔNp63的表達(dá)差異有統(tǒng)計(jì)學(xué)意義(X2=0.001,P0.05),在皮膚鱗狀細(xì)胞癌組中的表達(dá)明顯高于正常皮膚組。 鮑溫病和皮膚鱗狀細(xì)胞癌的曝光部位構(gòu)成比(曝光部位例數(shù)/總例數(shù)×100)比較(X2=0.000,P0.05)差異有統(tǒng)計(jì)學(xué)意義,皮膚鱗狀細(xì)胞癌發(fā)病以曝光部位為主。 鮑溫病中曝光部位及非曝光部位比較,ΔNp63的表達(dá)無(wú)統(tǒng)計(jì)學(xué)差異(X2=0.063,P0.05)。皮膚鱗狀細(xì)胞癌中曝光部位與非曝光部位比較,ΔNp63的表達(dá)無(wú)統(tǒng)計(jì)學(xué)差異(X2=0.212,P0.05)。 3、SPSS13.0統(tǒng)計(jì)軟件進(jìn)行相關(guān)分析：ΔNp63陽(yáng)性表達(dá)率與表皮中的表達(dá)模式*(P=0.000,P0.05)呈正相關(guān),表皮中彌漫表達(dá)模式的ΔNp63陽(yáng)性表達(dá)率高；ΔNp63陽(yáng)性表達(dá)率與鱗狀細(xì)胞癌分化程度(P=0.004,P0.05)呈正相關(guān),分化程度低的ΔNp63陽(yáng)性表達(dá)率高；ΔNp63陽(yáng)性表達(dá)率與曝光部位無(wú)相關(guān)性(P=0.966,P0.05)。 病種與年齡(P=0.000,P0.05)呈正相關(guān),隨年齡增加皮膚鱗狀細(xì)胞癌發(fā)病增加；病種與曝光部位(P=0.000,P0.05)有相關(guān)性,本研究調(diào)查發(fā)現(xiàn)鮑溫樣丘疹病和鮑溫病發(fā)病多在非曝光部位,皮膚鱗狀細(xì)胞癌發(fā)病多在曝光部位：病種與表皮中的表達(dá)模式*(P=0.000,P0.05)呈正相關(guān),鮑溫樣丘疹病多呈表皮基底層及表皮下1/3的細(xì)胞表達(dá)而鮑溫病呈表皮內(nèi)彌漫表達(dá)；病種與陽(yáng)性表達(dá)率(P=0.000,P0.05)呈正相關(guān),病種不同陽(yáng)性表達(dá)率也不同。 實(shí)驗(yàn)結(jié)論： 1、皮膚鱗狀細(xì)胞癌多發(fā)生在曝光部位,發(fā)病與紫外線照射有一定關(guān)系。 2、ΔNp63多考慮是未分化及低分化的上皮來(lái)源腫瘤的標(biāo)記。 3、ΔNp63可作為鑒別鮑溫樣丘疹病及鮑溫病的免疫學(xué)指標(biāo)。
[Abstract]:Objective:
1, to investigate the expression and significance of delta Np63 in normal skin, Bowen like papules, Bowen's disease, and skin squamous cell carcinoma.
2, to explore the immunological differential diagnosis of Bowen like papular disease and abalone fever.
Experimental methods:
Immunohistochemical Envision method was used to detect the expression of Np63, including 10 cases of normal skin, 30 cases of Bowen's papule disease, 30 cases of Bowen's disease, 30 cases of cutaneous squamous cell carcinoma,.PBS as a negative control.
Experimental results:
1, the expression of Np63 in 10 cases of normal skin tissue in the basal layer of the epidermis and epidermal 1/3 cells in 30 cases of Bowenoid papulosis tissues and 6 cases of skin diffuse expression, 24 cases of basal layer of the epidermis and epidermal 1/3 cells expression; in 30 cases of Bowen's disease in 1 cases. The expression of 29 cases of skin diffuse expression; expression of basal cells in the epidermis and dermis of 30 cases of skin squamous cell carcinoma tissue and carcinoma in the nest in the high differentiated squamous cell carcinoma (21 cases) of the cancer nest peripheral annular expression in squamous cell carcinoma (9 cases) was filled with nests.
2, SPSS13.0 statistical software for X2 test, a Np63 in Bowen's disease, a statistically significant expression of cutaneous squamous cell carcinoma group compared (X2=0.001, P0.05), the positive expression in Bowen's disease group was higher than that of cutaneous squamous cell carcinoma group. There was significant difference between the expression of Np63 in Bowenoid papulosis group and Bowen's disease in the group (X2=0.000, P0.05), the positive expression of Bowen's disease group was higher than that of Bowenoid papulosis group. Comparison of Np63 in squamous cell carcinoma group and Bowenoid papulosis group expression (X2=0.125, P0.05), no statistically significant difference. Comparison of expression of Np63 in Bowenoid papulosis group and normal skin in the group (X2=0.072, P0.05) had no significant difference. There was significant difference in the expression of normal skin and cutaneous squamous cell carcinoma group in a Np63 (X2=0.001, P0.05), the expression in cutaneous squamous cell carcinoma was significantly higher than normal Skin group.
The proportion of exposure sites of Bowen's disease and skin squamous cell carcinoma (exposure site number / total number of cases 100) was statistically significant (X2=0.000, P0.05). The incidence of cutaneous squamous cell carcinoma was mainly exposed.
There was no significant difference in the expression of delta Np63 between Bowen's disease and exposed parts. There was no significant difference in the expression of delta X2=0.063 (X2=0.063, P0.05). There was no significant difference in the expression of delta Np63 between the exposure site and the non exposed part of the skin squamous cell carcinoma (X2=0.212, P0.05).
3, SPSS13.0 statistical software for correlation analysis: the rate and patterns of expression in the epidermis. The positive expression of Np63 * (P=0.000, P0.05) were positively correlated in the epidermis of diffuse expression of delta Np63 expression pattern of high rate; a positive expression rate of Np63 and the degree of differentiation of squamous cell carcinoma (P=0.004, P0.05) was positively related to Np63 positive the expression of low degree of differentiation rate is high; a positive expression rate of Np63 and the exposure site no correlation (P=0.966, P0.05).
Disease and age (P=0.000, P0.05) was positively related to the incidence of squamous cell carcinoma of the skin increased with age; disease and exposure sites (P=0.000, P0.05) are related, the survey found that Bowenoid papulosis and Bowen's disease in non exposed parts, the incidence of skin squamous cell carcinoma in the exposure site: the expression pattern of disease and skin in * (P=0.000, P0.05) were positively correlated, Bowenoid papulosis is the expression of the basal layer of the epidermis and epidermal cells of 1/3 and Bowen's disease showed diffuse expression of epidermal diseases; and the positive expression rate (P=0.000, P0.05) were positively related to different diseases, the positive expression rate is also different.
Experimental conclusions:
1, skin squamous cell carcinoma occurs mostly at the exposure site, which has a certain relationship with ultraviolet radiation.
2, Delta Np63 is considered as a marker of undifferentiated and poorly differentiated epithelial derived tumors.
3, Delta Np63 can be used as an immunological index to differentiate abalone from papular disease and abalone fever.

【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R739.5

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