本文選題：嗜堿性粒細(xì)胞　切入點(diǎn)：P物質(zhì)　出處：《錦州醫(yī)科大學(xué)》2016年碩士論文

【摘要】：目的通過檢測慢性自發(fā)性蕁麻疹患者外周血中嗜堿性粒細(xì)胞SP和NK1R的表達(dá),以及SP對(duì)嗜堿性粒細(xì)胞的生物學(xué)功能和細(xì)胞募集的影響,闡明SP和嗜堿性粒細(xì)胞在慢性自發(fā)性蕁麻疹的發(fā)生發(fā)展中可能存在的作用,為理解慢性自發(fā)性蕁麻疹的發(fā)病機(jī)制提供新思路,為治療慢性自發(fā)性蕁麻疹提供新靶點(diǎn)。方法在本研究中,我們使用流式細(xì)胞儀分析檢測了慢性自發(fā)性蕁麻疹患者外周血嗜堿性粒細(xì)胞的SP和NK1R的表達(dá)以及嗜堿性粒細(xì)胞的數(shù)目;通過嗜堿性粒細(xì)胞激發(fā)試驗(yàn)研究SP對(duì)嗜堿性粒細(xì)胞組胺釋放的影響;通過小鼠致敏模型的實(shí)驗(yàn)方法分別檢測了小鼠外周血中嗜堿性粒細(xì)胞SP和NK1R的表達(dá)以及SP對(duì)嗜堿性粒細(xì)胞的功能影響。結(jié)果在CSU患者血漿SP水平和嗜堿性粒細(xì)胞數(shù)分別比HC受試者??更高。與HC受試者的外周血比較,CSU患者中SP+和NK1R+嗜堿性粒細(xì)胞的比例均顯著升高。加入SP后,可引發(fā)CSU患者嗜堿性粒細(xì)胞高達(dá)41.2%的凈組胺釋放率,其促進(jìn)作用甚至可與抗Ig E和f MLP的相比較。腹腔注射以后,SP可引發(fā)小鼠外周血嗜堿性粒細(xì)胞數(shù)目的顯著增加。OVA致敏小鼠比非致敏小鼠外周血具有更多SP+和NK1R+嗜堿性粒細(xì)胞。結(jié)論高水平的血漿SP濃度,高表達(dá)的嗜堿性粒細(xì)胞SP和NK1R,以及SP的誘導(dǎo)嗜堿性粒細(xì)胞脫顆粒和募集的能力,都強(qiáng)有力的表明,SP是一種潛在的促炎性因子。它通過作用于嗜堿性粒細(xì)胞在CSU的發(fā)病機(jī)制中發(fā)揮重要作用。SP的抑制劑和NK1R的阻斷劑可能會(huì)成為治療CSU的有效制劑。
[Abstract]:Objective to investigate the expression of basophil SP and NK1R in peripheral blood of patients with chronic spontaneous urticaria and the effects of SP on the biological function and cell recruitment of basophil. To elucidate the possible role of SP and basophil in the occurrence and development of chronic spontaneous urticaria and to provide new ideas for understanding the pathogenesis of chronic spontaneous urticaria. Methods in this study, we used flow cytometry to detect the expression of SP and NK1R and the number of basophil in peripheral blood of patients with chronic spontaneous urticaria. The effect of SP on histamine release from basophil was studied by basophil stimulation test. The expression of SP and NK1R in peripheral blood of mice and the effect of SP on the function of basophil were detected by mouse sensitization model. Results in patients with CSU, plasma SP level and basophil were detected. More than HC subjects? ? Compared with the peripheral blood of HC subjects, the percentage of SP and NK1R eosinophils in patients with CSU was significantly higher than that in patients with HC.After the addition of SP, the net histamine release rate of basophil was up to 41.2% in patients with CSU. After intraperitoneal injection, SP could induce a significant increase in the number of basophil in peripheral blood of mice. OVA-sensitized mice had more SP and NK1R eosinophils than non-sensitized mice. Conclusion the high level of plasma SP concentration, Overexpression of basophil SP and NK1R, and SP's ability to induce degranulation and recruitment of basophil, It is strongly suggested that SP is a potential proinflammatory factor, which may be an effective agent for the treatment of CSU by acting on basophil. The inhibitor of SP and the antagonist of NK1R may play an important role in the pathogenesis of CSU by acting on basophilic granulocytes.
【學(xué)位授予單位】：錦州醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R758.24

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