發(fā)布時(shí)間：2018-03-21 04:46

  本文選題：皮膚鱗狀細(xì)胞癌　切入點(diǎn)：成纖維細(xì)胞激活蛋白　出處：《鄭州大學(xué)》2010年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目的本研究檢測(cè)成纖維細(xì)胞激活蛋白和整合素β1在皮膚鱗狀細(xì)胞癌組織切片和正常皮膚切片中的表達(dá)情況,比較兩種蛋白表達(dá)與皮膚鱗狀細(xì)胞癌病理特征的關(guān)系,分析其表達(dá)之間的相關(guān)性,初步探討成纖維細(xì)胞激活蛋白和整合素β1在皮膚鱗狀細(xì)胞癌發(fā)生發(fā)展中的作用。 材料和方法 收集鄭州大學(xué)第一附屬醫(yī)院整形外科自2006年～2008年確診存檔的皮膚鱗狀細(xì)胞癌石蠟固定包埋的46例病理標(biāo)本。病變部位來(lái)自頭皮、面部、背部、四肢。所有患者術(shù)前均有詳細(xì)的病例記錄,未接受放射,激光,免疫等抗腫瘤治療。男性20例,女性26例,年齡26～92歲(平均55.2歲)。皮膚鱗狀細(xì)胞癌病理學(xué)分級(jí)：高分化、中分化、低分化。20例正常對(duì)照皮膚取自整形外科全厚皮片移植剩余皮片和美容手術(shù)切除皮膚。采用免疫組織化學(xué)方法進(jìn)行實(shí)驗(yàn)。應(yīng)用SPSS13.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)分析,P0.05為有統(tǒng)計(jì)學(xué)意義。 結(jié)果 1.成纖維細(xì)胞激活蛋白在正常皮膚組織中無(wú)明顯陽(yáng)性表達(dá)。在皮膚鱗狀細(xì)胞癌中成纖維細(xì)胞激活蛋白可見(jiàn)胞膜或胞質(zhì)內(nèi)呈明顯棕黃色顆粒,陽(yáng)性反應(yīng)產(chǎn)物主要分布于腫瘤間質(zhì)成纖維細(xì)胞的胞膜或胞質(zhì)內(nèi)。陽(yáng)性表達(dá)率為87%。高分化鱗癌與中分化鱗癌比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),中分化鱗癌與低分化鱗癌(P0.05),高分化鱗癌與低分化鱗癌比較差異有統(tǒng)計(jì)學(xué)意義(P0.05) 2.整合素β1在正常皮膚組織中呈現(xiàn)表面均質(zhì)性高表達(dá),陽(yáng)性染色主要定位在細(xì)胞膜表面,胞質(zhì)有少量表達(dá)。在皮膚鱗狀細(xì)胞癌的細(xì)胞膜中,腫瘤細(xì)胞及腫瘤間質(zhì)細(xì)胞中均有表達(dá)。陽(yáng)性表達(dá)率為52%。在高分化鱗癌中呈高表達(dá)或接近高表達(dá),在中低分化鱗癌細(xì)胞及間質(zhì)中表達(dá)降低甚至消失。高中分化鱗癌比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),高低分化鱗癌比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),中低分化鱗癌比較無(wú)統(tǒng)計(jì)學(xué)意義(P0.05) 3.成纖維細(xì)胞激活蛋白的陽(yáng)性表達(dá)與整合素β1的異常表達(dá)有關(guān)。二者的表達(dá)差異有統(tǒng)計(jì)學(xué)意義(P0.05)。隨成纖維細(xì)胞激活蛋白的表達(dá)加強(qiáng),整合素β1表達(dá)減弱。 結(jié)論 1.成纖維細(xì)胞激活蛋白在皮膚鱗狀細(xì)胞癌中表達(dá)而在正常皮膚中不表達(dá),且成纖維細(xì)胞激活蛋白隨皮膚鱗狀細(xì)胞癌惡性程度的增高表達(dá)增強(qiáng),提示成纖維細(xì)胞激活蛋白表達(dá)水平與腫瘤的惡性程度相關(guān)。 2.整合素β1在正常皮膚中呈均質(zhì)表達(dá),在皮膚鱗狀細(xì)胞癌中表達(dá)率較正常皮膚陽(yáng)性率降低,且整合素β1隨皮膚鱗狀細(xì)胞癌惡性程度的增高表達(dá)減弱。提示整合素β1的表達(dá)與腫瘤惡性程度呈負(fù)相關(guān)。 3.成纖維細(xì)胞激活蛋白表達(dá)與整合素β1表達(dá)異常,呈負(fù)相關(guān),提示成纖維細(xì)胞激活蛋白的過(guò)表達(dá)可伴有整合素β1表達(dá)減弱,提示兩者可能有共同的調(diào)節(jié)機(jī)制。 4.研究成纖維細(xì)胞激活蛋白與整合素β1在皮膚鱗狀細(xì)胞癌間質(zhì)中的表達(dá),有望使其成為皮膚鱗狀細(xì)胞癌治療的新靶點(diǎn)。
[Abstract]:Objective to investigate the expression of fibroblast activator protein (FAPP) and integrin 尾 1 (integrin 尾 1) in skin squamous cell carcinoma (SCC) tissues and normal skin sections, and to compare the relationship between the expression of fibroblast activator protein (FAPP) and the pathological features of SCC. To explore the role of fibroblast activator protein (FAPP) and integrin 尾 1 (integrin 尾 1) in the development of cutaneous squamous cell carcinoma (SCC). Materials and methods. A total of 46 paraffin embedded specimens of squamous cell carcinoma of skin were collected from plastic surgery department of the first affiliated Hospital of Zhengzhou University from 2006 to 2008. The lesions were located on the scalp, face and back. Extremities. All patients had detailed records of cases before operation and had not received anti-tumor therapy such as radiation, laser, immunity, etc. Male 20 cases, female 26 cases, age 2692 years (mean 55.2 years old). Pathological grade of cutaneous squamous cell carcinoma: highly differentiated. Moderate differentiation, Low differentiation. 20 cases of normal control skin were obtained from the residual skin grafts of plastic surgery and cosmetic surgery. Immunohistochemical method was used to carry out the experiment. Statistical analysis using SPSS13.0 statistical software was statistically significant. Results. 1.Fibroblast activator protein was not expressed in normal skin tissue, fibroblast activator protein could be seen in the membrane or cytoplasm of skin squamous cell carcinoma. The positive reaction products were mainly distributed in the cell membrane or cytoplasm of the tumor interstitial fibroblasts. The positive expression rate was 87%. There was no significant difference between well-differentiated squamous cell carcinoma and moderately differentiated squamous cell carcinoma (P0.05), and there was no significant difference between moderately differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma (P0.05). The difference between differentiated squamous cell carcinoma and poorly differentiated squamous cell carcinoma was statistically significant (P0.05). 2. Integrin 尾 1 was highly expressed on the surface of normal skin tissues. The positive staining was mainly located on the surface of the cell membrane, and a small amount of expression was found in the cytoplasm, and in the cell membrane of squamous cell carcinoma of skin, the expression of integrin 尾 1 was higher than that of the normal skin. Both tumor cells and stromal cells were expressed. The positive expression rate was 52%. There was high expression or close to high expression in highly differentiated squamous cell carcinoma (SCC). The expression decreased or even disappeared in middle and low differentiated squamous cell carcinoma cells and stroma. There was significant difference in high and middle differentiated squamous cell carcinoma (P 0.05), high differentiation squamous cell carcinoma (P 0.05), and middle and low differentiation squamous cell carcinoma (P 0.05), but there was no significant difference in middle and low differentiation squamous cell carcinoma (P 0.05), but there was no significant difference between middle and low differentiation squamous cell carcinoma (P 0.05). 3. The positive expression of fibroblast activator protein was related to the abnormal expression of integrin 尾 1. There was a significant difference between the expression of integrin 尾 1 and the expression of integrin 尾 1. The expression of integrin 尾 1 decreased with the increase of expression of fibroblast activator protein. Conclusion. 1. Fibroblast activator protein was expressed in cutaneous squamous cell carcinoma but not in normal skin, and the expression of fibroblast activator protein increased with the malignant degree of skin squamous cell carcinoma. The results suggest that the expression level of fibroblast activator protein is related to the malignancy of tumor. 2. The expression of integrin 尾 1 in normal skin was homogeneous, and the positive rate of integrin 尾 1 in skin squamous cell carcinoma was lower than that in normal skin. The expression of integrin 尾 1 decreased with the increase of malignant degree of cutaneous squamous cell carcinoma, suggesting that the expression of integrin 尾 1 was negatively correlated with the degree of malignancy of the tumor. 3. The expression of fibroblast activator protein was negatively correlated with the abnormal expression of integrin 尾 _ 1, suggesting that the overexpression of fibroblast activator protein may be accompanied by a decrease of integrin 尾 _ 1 expression, suggesting that there may be a common regulatory mechanism between them. 4. To study the expression of fibroblast activator protein and integrin 尾 1 in the interstitial tissue of cutaneous squamous cell carcinoma, which is expected to become a new target for the treatment of cutaneous squamous cell carcinoma.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R739.5

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