發(fā)布時(shí)間：2018-03-16 14:45

  本文選題：轉(zhuǎn)鐵蛋白　切入點(diǎn)：RGD　出處：《中國(guó)生化藥物雜志》2014年04期 　論文類型：期刊論文

【摘要】：目的構(gòu)建轉(zhuǎn)鐵蛋白(transferrin,TF)與RGD(精氨酸-甘氨酸-天冬氨酸,Arg-Gly-Asp)共修飾PLGA(聚乳酸羥基乙酸,poly(lactic-co-dycolic acid)納米粒(TF/RGD-NPs),研究其黑色素瘤靶向性。方法采用乳化法制備TF和RGD共修飾納米粒(TF/RGD-NPs),考察其形態(tài)、粒徑、電位等理化性質(zhì)。通過細(xì)胞攝取實(shí)驗(yàn)和黑色素瘤腫瘤球穿透實(shí)驗(yàn)考察TF/RGD-NPs與黑色素瘤B16細(xì)胞的親和力和腫瘤組織穿透能力。結(jié)果制備的TF/RGD-NPs粒徑為(113.4±12.5)nm,電位為(4.53±2.15)mV。體外細(xì)胞攝取實(shí)驗(yàn)表明B16細(xì)胞對(duì)TF/RGD-NPs的攝取效率分別是TF-NPs和RGD-NPs的2.7倍和2.9倍,差異均具有統(tǒng)計(jì)學(xué)意義(P0.01)。細(xì)胞攝取實(shí)驗(yàn)和腫瘤球攝取實(shí)驗(yàn)結(jié)果表明TF/RGD-NPs具有良好的黑色素瘤細(xì)胞親和力。結(jié)論轉(zhuǎn)鐵蛋白與RGD共修飾納米粒具有良好的黑色素瘤靶向性,是一種潛在的黑色素瘤靶向給藥系統(tǒng)。
[Abstract]:Objective to construct TFRGD-NPsC (transferrin) and RGD (arginine-glycine-aspartic acid) co-modified PLGA (poly lactic-co-dycolic acid) nanoparticles, and to study the melanoma targeting of TFRGD-NPs.Methods TF and RGD co-modified TFGD-NPsn were prepared by emulsification method, and their morphology was investigated. Particle size, The affinity of TF/RGD-NPs to melanoma B16 cells and the penetrating ability of tumor tissue were investigated by cell uptake test and tumor ball penetration test. The results showed that the diameter of TF/RGD-NPs was 113.4 鹵12.5 nm and the potential was 4.53 鹵2.15 MV in vitro. The uptake efficiency of TF/RGD-NPs in B16 cells was 2.7 times and 2.9 times higher than that of TF-NPs and RGD-NPs, respectively. The results of cell uptake test and tumor ball uptake test showed that TF/RGD-NPs had good melanoma cell affinity. Conclusion Transferrin and RGD co-modified nanoparticles have good melanoma targeting ability. It is a potential melanoma targeting drug delivery system.
【作者單位】： 華中農(nóng)業(yè)大學(xué)醫(yī)院;
【基金】：國(guó)家自然科學(xué)基金(81171365)
【分類號(hào)】：R739.5

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