本文關(guān)鍵詞： 銀屑病 HLA 蒙古族 相關(guān)性研究　出處：《內(nèi)蒙古醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過分析內(nèi)蒙古蒙古族尋常型銀屑病患者HLA-Cw及DRB1等位基因基因型頻率，研究蒙古族人群尋常型銀屑病與HLA-Cw及DRB1等位基因的相關(guān)性，探討尋常型銀屑病的致病基因并為銀屑病病因?qū)W研究提供理論依據(jù)。通過對蒙古族尋常型銀屑病與HLA相關(guān)性研究來豐富銀屑病易感基因圖譜，為今后銀屑病相關(guān)研究提供有價值資料，為銀屑病基因水平的診斷、治療、預(yù)后評估及相關(guān)研究奠定理論基礎(chǔ)。方法:本實驗研究選擇無血緣關(guān)系、3代以上連續(xù)生活在內(nèi)蒙古境內(nèi)的蒙古族人群，提取血液細(xì)胞基因組DNA，利用序列特異性引物聚合酶鏈反應(yīng)（PCR-SSP）對蒙古族尋常型銀屑病患者81例及正常蒙古族100例的HLA-Cw及DRB1位點等位基因進(jìn)行基因分型。根據(jù)HLA各等位基因在患者組和對照組之間頻率分布，利用SPSS13.0統(tǒng)計軟件進(jìn)行χ2檢驗，分析兩組間各等位基因頻率差異。結(jié)果:共檢出10個HLA-Cw座位等位基因，12個HLA-DRB1座位等位基因。對這些檢出的等位基因根據(jù)銀屑病組和正常人組基因頻率分布差異進(jìn)行統(tǒng)計學(xué)分析，得出有顯著性差異的等位基因是：HLA-Cw*04，Cw*06，DRB1*04和DRB1*07等位基因。其中，銀屑病組HLA-Cw*06，DRB1*07等位基因頻率顯著高于正常人組，HLA-Cw*04、DRB1*04等位基因頻率顯著低于正常人組（Pc＜0.05）。在發(fā)病年齡＜40歲銀屑病及家族史陰性患者中HLA-Cw*06、DRB1*07等位基因頻率顯著高于正常人組，，而HLA-Cw*04、DRB1*04等位基因頻率顯著低于正常人組（Pc＜0.05）。在發(fā)病年齡≥40歲的銀屑病及家族史陽性患者中只有HLA-Cw*06等位基因頻率顯著高于正常人組（Pc＜0.05）。HLA-Cw*04、DRB1*04基因頻率的下降及Cw*06、DRB1*07基因頻率的上升在無家族史銀屑病患者間均有統(tǒng)計學(xué)意義（Pc＜0.05），而Cw*06的基因頻率還在家族史陽性患者中有顯著上升（Pc＜0.05）。結(jié)論: HLA-Cw*06、DRB1*07等位基因可能是內(nèi)蒙古地區(qū)蒙古族人群尋常型銀屑病的易感基因。HLA-Cw*04、DRB1*04等位基因等位基因可能是內(nèi)蒙古地區(qū)蒙古族人群尋常型銀屑病發(fā)病的保護因子。HLA-DRB1*07等位基因可能是發(fā)病年齡＜40歲的銀屑病的易感基因，而HLA-Cw*04、DRB1*04等位基因則可能是發(fā)病年齡＜40歲銀屑病的保護因子。有銀屑病家族史與無銀屑病家族史在基因遺傳上有一定差別，而HLA-Cw*06等位基因可能是二者共同的遺傳基礎(chǔ)。
[Abstract]:Objective: through the analysis of Inner Mongolia Mongolian patients with psoriasis vulgaris and HLA-Cw DRB1 allele genotype frequency correlation of Mongolian people with psoriasis vulgaris HLA-Cw and DRB1 alleles, pathogenic genes of psoriasis vulgaris and to provide a theoretical basis for psoriasis etiology research. To enrich the susceptible gene of psoriasis by studying the map the relationship between psoriasis and HLA vulgaris of the Mongolian, for the future of psoriasis related research provide valuable information for the diagnosis, treatment of psoriasis gene level, prognosis evaluation and related research lays the theory. Methods: This study chose unrelated, more than 3 generations of continuous life in the territory of Inner Mongolia Mongolian population, extraction of blood cell genomic DNA by polymerase chain reaction sequence specific primers (PCR-SSP) for patients with Mongolian psoriasis vulgaris and 81 cases of normal Mongolia 100 cases of HLA-Cw and DRB1 alleles were genotyped. According to the frequency distribution of HLA allele between the patients group and control group, using statistical software SPSS13.0 2 test, analysis between the two groups of allele frequency differences. Results: there were 10 HLA-Cw alleles. 12 HLA-DRB1 alleles. These alleles were analyzed statistically according to the difference of the frequency distribution of the psoriasis group and normal group of genes that have significant differences in the allele is: HLA-Cw*04, Cw*06, DRB1*04 and DRB1*07 alleles. Among them, the psoriasis group HLA-Cw*06, DRB1*07 allele frequency was significantly higher than normal group, HLA-Cw*04, DRB1*04 allele frequency was significantly lower than that of normal group (Pc < 0.05). The age of onset of HLA-Cw*06 in less than 40 years of family history of psoriasis and negative patients, the DRB1*07 allele frequency was significantly higher than that of The normal group, while the HLA-Cw*04, DRB1*04 allele frequency was significantly lower than that of normal group (Pc < 0.05). In psoriasis and a positive family history of patients aged 40 years and only the HLA-Cw*06 allele frequency was significantly higher than that in normal group (Pc < 0.05).HLA-Cw*04, decrease of Cw*06 and DRB1*04 gene frequency, DRB1*07 the gene frequency of rise in the no family history of psoriasis patients were statistically significant (Pc < 0.05), and a family history of gene frequency in patients with positive Cw*06 was significantly increased (Pc < 0.05). Conclusion: HLA-Cw*06, DRB1*07 allele may be the susceptibility to psoriasis vulgaris in Mongolian population in Inner Mongolia region of.HLA-Cw*04 gene and the DRB1*04 allele may be the pathogenesis of vulgaris psoriasis in Inner Mongolia area of the Mongolian population protection factor.HLA-DRB1*07 allele may be a susceptible age < 40 years old psoriasis gene, HLA-Cw*04 and DRB1*04 alleles may be protective factors of psoriasis at onset age less than 40 years old. Family history of psoriasis is different from that of family history without psoriasis, and HLA-Cw*06 allele may be the common genetic basis of the two.

【學(xué)位授予單位】：內(nèi)蒙古醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R758.63

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相關(guān)期刊論文 前9條

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本文編號：1553343