【摘要】：目的探討雌激素對(duì)尾懸吊導(dǎo)致雄性C57小鼠骨質(zhì)疏松的影響及其內(nèi)在機(jī)制。方法 32只成年C57BL/6J雄性小鼠,隨機(jī)分成4組:對(duì)照組(control,C),模型組(model,M),雌激素組(estrogen,E),雌激素+雌激素受體抑制組(estrogen+ICI 182,780,E+I)。除C組外其余組小鼠尾懸吊兩周,所有小鼠在第15d處死,取小鼠血清進(jìn)行生化指標(biāo)分析,取小鼠右側(cè)股骨進(jìn)行顯微CT掃描和形態(tài)學(xué)染色,取小鼠左側(cè)股骨進(jìn)行生物力學(xué)檢測(cè)并取股骨遠(yuǎn)端研磨后進(jìn)行蛋白檢測(cè)。結(jié)果尾懸吊兩周導(dǎo)致雄性C57小鼠股骨出現(xiàn)明顯骨質(zhì)疏松,M組與C組相比差異有顯著的統(tǒng)計(jì)學(xué)意義(P0.05)。雌激素干預(yù)能抑制骨質(zhì)流失,E組多數(shù)骨相關(guān)參數(shù)與對(duì)照組相比,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。而使用雌激素受體拮抗劑ICI182,780之后,雌激素骨保護(hù)效應(yīng)消失,與M組相比差異無統(tǒng)計(jì)學(xué)意義(P0.05)。尾懸吊兩周導(dǎo)致股骨遠(yuǎn)端離子型谷氨酸受體NMDA亞型NR2A表達(dá)降低,雌激素升高骨組織中NR2A表達(dá)水平。結(jié)論雄性C57小鼠尾懸吊2w表現(xiàn)出顯著骨質(zhì)疏松,雌激素通過激活雌激素受體明顯改善骨質(zhì)流失,可能是通過促進(jìn)骨組織中NR2A表達(dá)而實(shí)現(xiàn)成骨細(xì)胞增殖和分化。
[Abstract]:Objective to investigate the effect of estrogen on osteoporosis induced by tail suspension in male C57 mice and its underlying mechanism. Methods 32 adult C57BL/6J male mice were randomly divided into 4 groups: control group (control,C), model group (model,M), estrogen group (estrogen,E), estrogen receptor inhibition group (estrogen ICI 182, 780, E I). The mice in the other groups were suspended for two weeks except group C, all the mice were killed on the 15th day, the serum of the mice was taken for biochemical index analysis, and the right femur of the mice was taken for microscopic CT scanning and morphological staining. The left femur of the mice was taken for biomechanical examination and the distal femur was ground for protein detection. Results two weeks of tail suspension resulted in significant osteoporosis in the femur of male C57 mice, and there was significant difference between M group and C group (P0.05). Estrogen intervention can inhibit bone loss, E group compared with the control group, most of the bone-related parameters, there is no significant difference (P0.05). When estrogen receptor antagonist ICI182780 was used, the bone protective effect of estrogen disappeared, and there was no significant difference compared with M group (P0.05). Tail suspension for two weeks resulted in the decrease of NR2A expression of ionized glutamate receptor NMDA subtype in distal femur and the elevation of NR2A expression in bone tissue by estrogen. Conclusion male C57 mice showed significant osteoporosis after 2 weeks of tail suspension. Estrogen could significantly improve bone loss by activating estrogen receptor, which might be related to the proliferation and differentiation of osteoblasts by promoting the expression of NR2A in bone tissue.
【作者單位】： 南京大學(xué)醫(yī)學(xué)院附屬金陵醫(yī)院骨科;第四軍醫(yī)大學(xué)基礎(chǔ)部藥理學(xué)教研室;
【基金】：江蘇省六大人才高峰資助(2013-WSN-091) 江蘇省臨床科技項(xiàng)目基金(BL2012002)
【分類號(hào)】：R580

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