發(fā)布時(shí)間：2018-11-14 10:03

【摘要】：目的探討高糖對(duì)腎小球系膜細(xì)胞細(xì)胞外基質(zhì)表達(dá)的影響,以及西格列汀(SIT)對(duì)其的干預(yù)作用。方法培養(yǎng)腎小球系膜細(xì)胞,分為對(duì)照(Con)組、高糖(HG)組,以及高糖聯(lián)合不同濃度的SIT組(HG+S1組、HG+S5組、HG+S10組和HG+S20組)。培養(yǎng)24 h、48 h后,用MTT法測(cè)定細(xì)胞增殖程度,ELISA檢測(cè)細(xì)胞培養(yǎng)上清層粘連蛋白(LN)、大鼠Ⅳ型膠原蛋白(Col-Ⅳ)、轉(zhuǎn)化生長因子β1(TGF-β1)蛋白表達(dá)的變化。結(jié)果高糖培養(yǎng)24 h、48 h后,可觀察到不同濃度的SIT均可抑制高糖誘導(dǎo)的系膜細(xì)胞增殖;SIT組與HG組比較,差異有統(tǒng)計(jì)學(xué)意義;Con組腎小球系膜細(xì)胞可分泌一定量的LN、Col-Ⅳ和TGF-β1,高糖刺激后LN、Col-Ⅳ和TGF-β1分泌增加,而SIT對(duì)系膜細(xì)胞分泌物的抑制隨其濃度的升高而增強(qiáng)。結(jié)論SIT可抑制高糖條件下系膜細(xì)胞的增殖,且能降低高糖條件下LN、Col-Ⅳ、TGF-β1的表達(dá),SIT可能對(duì)糖尿病患者的腎臟有一定的保護(hù)作用。
[Abstract]:Objective to investigate the effect of high glucose on the expression of extracellular matrix in glomerular Mesangial cells and the intervention of siglitatin (SIT). Methods glomerular Mesangial cells were cultured and divided into control (Con) group, high glucose (HG) group and SIT group with high glucose concentration (HG S1 group, HG S5 group, HG S10 group and HG S20 group). After cultured for 24 h or 48 h, the degree of cell proliferation was measured by MTT assay. The expression of type 鈪，

本文編號(hào)：2330852