【摘要】：目的:觀察跑臺(tái)運(yùn)動(dòng)對(duì)高脂飲食誘導(dǎo)的肥胖大鼠骨骼肌毛細(xì)血管生成及apelin/APJ表達(dá)的影響。方法:5周齡健康雄性SD大鼠隨機(jī)分成對(duì)照組和高脂喂養(yǎng)組,分別進(jìn)行普通飼料和高脂飼料喂養(yǎng),12周后篩選肥胖大鼠16只,隨機(jī)分為高脂安靜組和高脂運(yùn)動(dòng)組。跑臺(tái)訓(xùn)練持續(xù)10周,每周5次,每次60分鐘,跑速26米/分鐘。測(cè)定大鼠體重、體脂重及血脂水平,免疫組化法觀察比目魚肌毛細(xì)血管密度和apelin蛋白表達(dá)水平,放免法測(cè)定比目魚肌apelin含量,real-time PCR方法測(cè)定比目魚肌apelin和APJ的m RNA表達(dá)。結(jié)果:與對(duì)照組相比,高脂飲食喂養(yǎng)大鼠體重和體脂顯著增加(P0.01),總甘油三酯、總膽固醇和低密度脂蛋白膽固醇水平顯著升高,高密度脂蛋白膽固醇水平顯著降低(P0.05)。與對(duì)照組比,肥胖大鼠比目魚肌毛細(xì)血管生成無(wú)顯著變化,apelin和APJ m RNA表達(dá)無(wú)顯著變化(P0.05)。與單純高脂飲食組大鼠相比,10周跑臺(tái)運(yùn)動(dòng)大鼠體重顯著降低(P0.01),血脂改善;此外,比目魚肌毛細(xì)血管密度顯著升高,apelin和APJ m RNA表達(dá)顯著上升(P0.05),apelin蛋白表達(dá)水平顯著升高(P0.05)。結(jié)論:跑臺(tái)運(yùn)動(dòng)能顯著增加肥胖大鼠比目魚肌毛細(xì)血管生成,其作用可能與運(yùn)動(dòng)誘導(dǎo)比目魚肌apelin表達(dá)相關(guān)。
[Abstract]:Aim: to observe the effect of treadmill exercise on capillary formation and apelin/APJ expression in skeletal muscle of obese rats induced by high fat diet. Methods: five week-old healthy male SD rats were randomly divided into control group and high fat feeding group. After 12 weeks, 16 obese rats were selected and randomly divided into high fat quiet group and high fat exercise group. Treadmill training lasts 10 weeks, 5 times a week for 60 minutes, running at a speed of 26 m / min. Body weight, body fat weight and blood lipid level were measured, capillary density and apelin protein expression in soleus muscle were observed by immunohistochemical method, apelin content in soleus muscle was measured by radioimmunoassay, m RNA expression of apelin and APJ in soleus muscle was detected by real-time PCR method. Results: compared with the control group, the body weight and body fat of rats fed on high fat diet were significantly increased (P0.01), and the levels of total triglyceride, total cholesterol and low density lipoprotein cholesterol were significantly increased. The level of high density lipoprotein cholesterol decreased significantly (P0.05). Compared with the control group, there was no significant change in the expression of apelin and APJ m RNA in the flounder muscle of obese rats (P0.05). Compared with the high-fat diet group, the rats in the 10-week treadmill exercise group had significantly lower body weight (P0.01) and improved blood lipids. In addition, the density of capillaries in soleus muscle increased significantly, and the expression of apelin and APJ m RNA increased significantly (P0.05). Conclusion: treadmill exercise can significantly increase the formation of capillaries in the soleus muscle of obese rats. The effect of treadmill exercise may be related to the expression of apelin in the soleus muscle induced by exercise.
【作者單位】： 北京師范大學(xué)體育與運(yùn)動(dòng)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31471137)
【分類號(hào)】：R589.2
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本文編號(hào)：2329420