【摘要】：目的觀察重組減毒沙門菌作為胰高血糖素樣肽-1(GLP-1)的基因載體,對2型糖尿病小鼠的血糖調(diào)控作用。方法高脂飼料飼喂4周后予以鏈脲佐菌素(STZ)腹腔注射構(gòu)建KM種小鼠2型糖尿病模型,小鼠分為4組,每組10只,即:正常組、模型組、空載組及治療組,分別予以:5%NaHCO_3、5%NaHCO_3、同等劑量的空載減毒沙門菌及GLP-1重組減毒沙門菌灌胃治療,觀察并記錄治療前后第0、7、14、21、28天的空腹血糖值及進食量、飲水量、體重變化,分別于治療后第14天及第28天行口服葡萄糖耐量試驗(OGTT)。取小鼠胰腺組織,采用HE染色法觀察各組小鼠的胰腺組織形態(tài)學變化。結(jié)果 GLP-1重組減毒沙門菌灌胃治療后,模型組小鼠空腹血糖水平較正常組升高,差異有統(tǒng)計學意義(P0.01),空載組空腹血糖與模型組比較,差異無統(tǒng)計學意義,治療組空腹血糖與空載組比較降低,差異有統(tǒng)計學意義(P0.01),糖尿病小鼠體重變化有明顯改善(P0.01),多飲多食癥狀好轉(zhuǎn);第14天及第28天OGTT示:空載組血糖與模型組比較,差異無統(tǒng)計學意義,治療組血糖較空載組降低,差異有統(tǒng)計學意義(P0.01)。胰腺HE染色結(jié)果示,空載組與模型組比較,無明顯變化,治療組與空載組比較有明顯改善。結(jié)論 GLP-1重組減毒沙門菌能夠改善2型糖尿病小鼠多飲、多食、體重下降等癥狀,降低血糖,改善胰島形態(tài)學變化,對2型糖尿病小鼠具有一定的治療作用。
[Abstract]:Objective to observe the effect of recombinant attenuated Salmonella mutans on blood glucose in type 2 diabetic mice as a gene vector of glucagon like peptide 1 (GLP-1). Methods the type 2 diabetic model of KM mice was established by intraperitoneal injection of streptozotocin (STZ) in high fat diet for 4 weeks. The mice were divided into 4 groups with 10 mice in each group: normal group, model group, no-load group and treatment group. The rats were treated with 5% NaHCOS and 5 NaHCOs respectively. The same dose of non-loaded salmonella and GLP-1 recombinant attenuated salmonella were given intragastrically respectively. The fasting blood glucose value, the amount of food intake, the quantity of water consumed and the changes of body weight before and after treatment were observed and recorded. Oral glucose tolerance test (OGTT) (OGTT). Was administered on day 14 and day 28 after treatment, respectively. HE staining was used to observe the histomorphologic changes of the pancreas of the mice. Results after administration of GLP-1 recombinant attenuated Salmonella, the fasting blood glucose level in the model group was significantly higher than that in the normal group (P0.01), but there was no significant difference between the no-load group and the model group. The fasting blood glucose in the treatment group was significantly lower than that in the no-load group (P0.01), the weight change of diabetic mice was significantly improved (P0.01), and the symptoms of excessive drinking and eating were improved. OGTT on the 14th and 28th day showed that the blood glucose in the no-load group was higher than that in the model group. There was no significant difference in blood glucose in the treatment group compared with the no-load group, the difference was statistically significant (P0.01). The results of pancreatic HE staining showed that there was no significant change between the no-load group and the model group, but there was significant improvement in the treatment group and the no-load group. Conclusion GLP-1 recombinant attenuated Salmonella can improve the symptoms of type 2 diabetes, such as excessive drinking, eating and weight loss, decrease blood sugar, improve the morphological changes of pancreatic islets, and has a certain therapeutic effect on type 2 diabetic mice.
【作者單位】： 安徽醫(yī)科大學第三附屬醫(yī)院內(nèi)分泌科;上海交通大學Bio-x研究院上海交通大學實驗動物中心;
【基金】：國家自然科學基金(編號:30840106)
【分類號】：R587.1
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本文編號：2248659