發(fā)布時(shí)間：2018-08-14 19:04

【摘要】：為評(píng)價(jià)以玻璃為載體的微陣列芯片法對(duì)ENA抗體檢測(cè)的靈敏度和特異度,及其在常見(jiàn)自身免疫性疾病中的臨床應(yīng)用價(jià)值,我們以免疫印跡法(Western blotting,WB)和以玻璃為載體的微陣列芯片法(Microarray)分別對(duì)508例自身免疫性疾病患者和155例健康體檢者的血清樣本進(jìn)行了檢測(cè),并與WB比較。結(jié)果顯示:與WB方法相比,玻璃載體微陣列芯片法檢測(cè)的結(jié)果如下:Sm/nRNP靈敏度為98.95%,特異度為99.82%;Sm靈敏度為97.18%,特異度為99.49%;SSA靈敏度為95.29%,特異度為98.73%;SSB靈敏度為96.10%,特異度為99.83%;Scl-70靈敏度為98.48%,特異度為99.66%;PM-Scl靈敏度為100%,特異度為99.49%;Jo-1靈敏度為100%,特異度為99.67%;CENP-B靈敏度為98.39%,特異度為99.67%;PCNA靈敏度為98.33%,特異度為99.83%;dsDNA靈敏度為95.28%,特異度為99.64%;核小體靈敏度為96.70%,特異度為99.30%;組蛋白靈敏度為100%,特異度為98.16%;核糖體P蛋白靈敏度為92.50%,特異度為99.83%;AMA-M2靈敏度為92.31%,特異度為99.67%。從檢測(cè)結(jié)果來(lái)看,陽(yáng)性一致率為97.11%,陰性一致率為99.49%。微陣列芯片法與WB結(jié)果具有高度的一致性。用微陣列芯片法可同時(shí)檢測(cè)14項(xiàng)ENA抗體譜,操作簡(jiǎn)單、速度快、靈敏度高、特異性強(qiáng),具有完成多種項(xiàng)目同時(shí)檢測(cè)的作用,結(jié)果可用儀器和軟件來(lái)自動(dòng)判讀,值得臨床推廣應(yīng)用。
[Abstract]:To evaluate the sensitivity and specificity of glass microarray assay for detection of ENA antibody and its clinical application in common autoimmune diseases. The serum samples of 508 patients with autoimmune diseases and 155 healthy controls were detected by Western blotting wideband and microarray (Microarray) with glass as carrier, respectively, and compared with WB. The results show that: compared with WB method, The results of the glass carrier microarray method are as follows: the sensitivity is 98.95, the specificity is 99.822, the sensitivity is 97.18, the specificity is 99.499.29, the specificity is 98.7375, the sensitivity is 96.10, the specificity is 99.83Scl-70, the sensitivity is 98.48, the specificity is 99.66 and the sensitivity of PM-Scl is 99.66. The sensitivity of SSA is 95.29, the specificity of 98.7375 is 96.10, the specificity of 99.83Scl-70 is 98.48, the specificity of 99.66 is 99.66 and the sensitivity of SSA is 95.29, and the specificity of 99.83Scl-70 is 98.48. The sensitivity of CENP-B is 98.39, the sensitivity of PCNA is 98.33, the specificity of PCNA is 98.33, the sensitivity of specificity is 99.83dsDNA 95.28, the specificity is 99.64; the sensitivity of nucleosome is 96.70, the specificity is 99.300.The sensitivity of histone is 100, the specificity is 99.83dsDNA, the sensitivity of nucleosome is 96.70, and the specificity is 99.30.The sensitivity of PCNA is 98.33, the specificity is 95.28, the specificity is 99.64; the sensitivity of nucleosome is 96.70, the specificity is 99.30; the sensitivity of histone is 100. The sensitivity of ribosomal P protein was 92.50, the specificity was 99.83 and the sensitivity of AMA-M2 was 92.31, the specificity of ribosomal P protein was 99.67. The positive rate was 97.11% and the negative rate was 99.49%. The microarray chip method has high consistency with WB results. 14 ENA antibody spectra can be detected simultaneously by microarray chip method. The method is simple, fast, sensitive and specific. It has the function of simultaneous detection of various items. The results can be read automatically by means of instruments and software. It is worth popularizing and applying in clinic.
【作者單位】： 湖南省人民醫(yī)院檢驗(yàn)科;甘肅省人民醫(yī)院檢驗(yàn)科;深圳市賽爾生物技術(shù)有限公司研發(fā)部;
【基金】：湖南省科技廳廳基金項(xiàng)目(2013TT2040)
【分類號(hào)】：R446.6;R593.2

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