本文選題：MT1A基因 + MT2A基因��； 參考：《昆明醫(yī)科大學》2017年碩士論文

【摘要】：[目的]探討MT1A基因rs8052394多態(tài)性及MT2A基因rs28366003多態(tài)性與昆明地區(qū)漢族2型糖尿病(Type 2 diabetes mellitus,T2DM)的相關性。[方法]收集257例T2DM患者和108例健康對照(NC)者的外周靜脈血,采用Taqman實時熒光定量PCR技術對兩組MT1A基因rs8052394多態(tài)性及MT2A基因rs28366003多態(tài)性進行檢測,并比較分析各組基因型、等位基因頻率、風險因素及相關臨床和生化指標。[結果](1) 一般資料及生化資料在T2DM組與健康對照組間的比較:T2DM組Cr、UA、TG、FBG、BMI、HbA1C 均高于 NC 組(p0.05),而 HDL-C 低于 NC 組(p0.05),差異有統(tǒng)計學意義;其余指標兩組間差異無統(tǒng)計學意義(p0. 05)。(2)MT1A基因rs8052394多態(tài)性在T2DM與健康對照組間的比較:各組樣本基因型頻率均符合Hardy-Weinberg遺傳平衡定律(P0. 05),其中A等位基因頻率0. 71;G等位基因頻率0. 29。T2DM組的A/A基因型頻率高于NC組(X2=20. 519,p0. 001 );T2DM組A等位基因頻率高于NC組(X2=29.213,p0.001)。(3) MT2A基因rs28366003多態(tài)性在T2DM與健康對照組間的比較:各組樣本基因型頻率均符合Hardy-Weinberg遺傳平衡定律(P0. 05),其中A等位基因頻率0.76; G等位基因頻率0. 24。T2DM組的G/G基因型頻率高于NC組(X2=7.167, p=0.007), A/A基因型與A/G基因型頻率在T2DM與健康對照組之間無統(tǒng)計學差異(X2=4. 938,p=0. 026; X2=2. 877, p=0. 090); T2DM組G等位基因頻率高于NC組,統(tǒng)計學有差異(X2=1 1. 073, p = 0. 001 )。(4)MT1A 基因 rs8052394 及 MT2A 基因 rs28366003多態(tài)性在男性及女性間的比較均無統(tǒng)計學差異(P0. 05)。(5)二分類Logistic回歸分析提示:MT2A基因rs28366003多態(tài)性可能與2型糖尿病的發(fā)生無關,而高尿酸(UA)及MT1Ars8052394 A/A基因型可能是2型糖尿病發(fā)生的危險因素,HDL-C可能是2型糖尿病發(fā)生的保護因素。[結論]在昆明地區(qū)漢族人群中MT2A基因rs28366003多態(tài)性可能與2型糖尿病的發(fā)生無關,而MT1A rs8052394 A/A基因型及高尿酸(UA)可能是2型糖尿病發(fā)生的危險因素,高密度脂蛋白膽固醇(HDL-C)可能是2型糖尿病發(fā)生的保護因素。[目的]探討MT1A基因及MT2A基因與昆明地區(qū)漢族人群糖尿病慢性腎臟疾病(diabetic kidney disease, DKD)的相關性。[方法]應用Taqman探針技術對研究對象的MT1A基因(rs8052394)及MT2A基因(rs28366003)多態(tài)性進行檢測,并比較分析DKD的T2DM組(DKD組)(89例),無DKD的T2DM組(DKD0組)(168例)及健康對照組(NC組)(108例)各組間基因型和等位基因頻率以及相關臨床和生化指標的相關關系。[結果](1) 一般資料及生化資料在DKD0組、DKD組與NC組的比較:DKD組Cr、UA、FBG、HbA1C 均高于 NC 組及 DKD0組;DKD 組及 DKD。組 TG、BMI 均高于 NC組,HDL-C均低于NC組;DKD。組Cr、UA、FBG、HbA1C均高于NC組,差異有統(tǒng)計學意義(P0.05),其余指標兩組間差異無統(tǒng)計學意義(p0.05)。(2) MT1A基因rs8052394多態(tài)性在DKD0組、DKD組與健康對照組間的比較:各組樣本基因型頻率均符合Hardy-Weinberg遺傳平衡定律(P0.05) 其中A等位基因頻率0. 71;G等位基因頻率0.29。各組間基因型頻率及等位基因頻率均無統(tǒng)計學差異(p0.05)。(3)MT2A基因rs28366003多態(tài)性在DKD0組、DKD組與健康對照組間的比較:各組樣本基因型頻率均符合Hardy-Weinberg遺傳平衡定律(P0.05),其中A等位基因頻率0.76; G等位基因頻率0.24。DKD組G/G基因型頻率高于NC 組及 DKD0組(X2=17. 468,p0. 001; X2=10. 286, p=0. 001)。DKD 組 MT2A 基因rs28366003位點G等位基因頻率高于NC組及DKD0組,差異有統(tǒng)計學意義(X2=12. 637, p0. 001)。(4)二分類 Logistic 回歸分析提示:MT1A 基因rs8052394多態(tài)性可能與DKD的發(fā)生無關,而高TG、高HbA1C及MT2A基因rs28366003 G/G基因型為DKD發(fā)生的獨立危險因素。[結論]在昆明地區(qū)漢族人群中MT1A基因rs8052394多態(tài)性可能與DKD的發(fā)生無關,而高TG、高HbA1C及MT2A基因rs28366003 G/G基因型可能為DKD發(fā)生的危險因素。[目的]探討MT1A基因及MT2A基因與昆明地區(qū)漢族2型糖尿病合并外周動脈粥樣硬化(atherosclerosis,AS)的相關性。[方法]應用Taqman探針技術對研究對象的MT1A基因(rs8052394)及MT2A基因(rs28366003)多態(tài)性進行檢測,并比較分析的2型糖尿病合并外周動脈粥樣硬化組(AS) (87例),單純T2DM組(T2DM) (170例)及健康對照組(NC) (108例)各組間基因型和等位基因頻率以及相關臨床和生化指標的相關關系。[結果](1)—般資料及生化資料在T2DM組、AS組與NC組間的比較:AS組及 T2DM 組 Cr、UA、TG、FBG、BMI、HbA1C 均高于 NC 組,HDL-C 低于 NC 組,差異有統(tǒng)計學意義(P0.05);其余各組間兩兩比較差異無統(tǒng)計學意義(P0.05)。(2)MT1A基因rs8052394多態(tài)性在T2DM組、AS組與NC的比較:各組樣本基因型頻率均符合Hardy-Weinberg遺傳平衡定律(P0.05),其中A等位基因頻率0. 71; G等位基因頻率0.29。AS組及T2DM組MT1A基因rs8052394位點A/A基因型頻率高于 NC 組(X2=18.942, p0.001; X2=28.355, p0.001) ; AS 組及T2DM組間各基因型頻率均無統(tǒng)計學差異(X2=4.388, p=0. 111)。AS組及T2DM組MT1A基因rs8052394位點A等位基因頻率高于NC組(X2=17. 991, p0. 001;X2=27.823, p0.001) ; AS組及T2DM組間各等位基因頻率均無統(tǒng)計學差異(X2=3. 876, p=0. 049 )。( 3 ) MT2A 基因 rs28366003 多態(tài)性在 T2DM 組、AS 組與NC組間的比較:各組樣本基因型頻率均符合Hardy-Weinberg遺傳平衡定律(P0.05),其中A等位基因頻率0. 76; G等位基因頻率0. 24。T2DM組MT2A基因rs28366003位點G/G基因型高于NC組(X2=16.601,p0.001);其余各組間基因型頻率均無統(tǒng)計學差異(X2=1.293, p=0.524; X2=0, p=1)。AS組及NC組 A 等位基因頻率高于 T2DM 組(X2=17. 266, p0.001,X2=9. 082,p=0.003)。(4)二分類Logistic回歸分析提示:MT2A基因rs28366003多態(tài)性可能與2型糖尿病合并外周動脈粥樣硬化的發(fā)生無關,HDL-C(OR值-1. 43, 95%CI: 0. 10-0. 59)可能為2型糖尿病合并外周動脈粥樣硬化發(fā)生的保護性因素,MT1A基因rs8052394 A/A基因型可能為2型糖尿病合并外周動脈粥樣硬化發(fā)生的獨立危險因素。[結論]在昆明地區(qū)漢族人群中MT2A基因rs28366003多態(tài)性可能與2型糖尿病合并外周動脈粥樣硬化的發(fā)生無關,而MT1A基因rs8052394 A/A基因型可能為2型糖尿病合并外周動脈粥樣硬化發(fā)生的獨立危險因素。
[Abstract]:[Objective] to investigate the association of MT1A gene rs8052394 polymorphism and MT2A gene rs28366003 polymorphism with type 2 diabetes mellitus (Type 2 diabetes mellitus, T2DM) in Kunming Han nationality. [Methods] the peripheral venous blood of 257 cases of T2DM and 108 healthy controls (NC) were collected, and two groups of genes were selected by Taqman real-time fluorescent quantitative PCR. State and MT2A gene rs28366003 polymorphism were detected, and the genotype, allele frequency, risk factors and related clinical and biochemical indexes were compared and analyzed. [results] the general data and biochemical data were compared between the T2DM group and the healthy control group: Cr, UA, TG, FBG, BMI, HbA1C were higher than the NC group (P0.05) in the T2DM group. Group (P0.05), the difference was statistically significant, the difference between the two groups was not statistically significant (p0. 05). (2) the rs8052394 polymorphism of MT1A gene was compared between the T2DM and the healthy control groups: the genotype frequencies of each group were in accordance with the Hardy-Weinberg genetic balance law (P0. 05), including the A allele frequency 0.71, and the G allele frequency 0. 29.T2DM group. The frequency of A/A genotype was higher than that in NC group (X2=20. 519, p0. 001), and the frequency of A allele in T2DM group was higher than that in NC group (X2=29.213, p0.001). (3) rs28366003 polymorphism of MT2A gene was compared between T2DM and healthy controls: the genotype frequencies of each group were in conformity with the law of equilibrium (05), among which the allele frequency was 0.76; The frequency of G/G genotypes in group 0. 24.T2DM was higher than that in group NC (X2=7.167, p=0.007), and there was no statistical difference between A/A genotype and A/G genotype frequency between T2DM and healthy controls (X2=4. 938, p=0. 026, X2=2. 877, p=0. 090). There was no significant difference in the rs28366003 polymorphism of s8052394 and MT2A gene between men and women (P0. 05). (5) two classification Logistic regression analysis suggested that the rs28366003 polymorphism of the MT2A gene may not be related to the occurrence of type 2 diabetes, and the high uric acid (UA) and MT1Ars8052394 A/A genotypes may be a risk factor for the occurrence of type 2 diabetes. HDL-C may be a protective factor for the occurrence of type 2 diabetes. [Conclusion] the rs28366003 polymorphism of MT2A gene in the Han population in Kunming region may not be related to the occurrence of type 2 diabetes, while the MT1A rs8052394 A/A genotype and high uric acid (UA) may be a risk factor for type 2 diabetes, and high density lipoprotein cholesterol (HDL-C) may be type 2 diabetes mellitus [Objective] to investigate the correlation between MT1A gene and MT2A gene and diabetic kidney disease (DKD) in the Han population of Kunming. [Methods] the Taqman probe technique was used to detect the polymorphism of MT1A gene (rs8052394) and MT2A gene (rs28366003) of the subjects and to compare and analyze the DKD. 2DM group (group DKD) (group DKD), no DKD T2DM group (group DKD0) (group DKD0) and healthy control group (group NC) (108 cases) the correlation of genotype and allele frequency and related clinical and biochemical indexes. [results] general data and biochemical data in group DKD0, DKD group and NC group: DKD group Cr, UA, etc. DKD group and DKD. group TG, BMI were higher than group NC, HDL-C was lower than group NC; DKD. group Cr, UA, FBG, HbA1C were higher than those in the group, the difference was statistically significant. (2) the difference between the two groups was not statistically significant. Hardy-Weinberg genetic equilibrium law (P0.05) in which the A allele frequency was 0.71; the frequency of G allele frequencies and allele frequencies were not statistically different (P0.05). (3) the MT2A gene rs28366003 polymorphism was compared between the DKD0 group, the DKD group and the healthy control group: the genotype frequencies of each group were all consistent with the Hardy-Weinb. ERG genetic balance (P0.05), A allele frequency 0.76, G allele frequency 0.24.DKD group G/G genotype frequency is higher than the NC group and DKD0 group (X2=17. 468, p0. 001; X2=10. 286, p=0. 001) allele frequencies are higher than that of the group and the group, the difference is statistically significant (4). Two Logistic regression analysis suggests that the rs8052394 polymorphism of the MT1A gene may not be related to the occurrence of DKD, while high TG, high HbA1C and MT2A gene rs28366003 G/G genotype are independent risk factors for DKD. [Conclusion] the MT1A gene rs8052394 polymorphism in the Han population in Kunming region can be independent of the occurrence of DKD. The A gene rs28366003 G/G genotype may be a risk factor for the occurrence of DKD. [Objective] to investigate the correlation between the MT1A gene and the MT2A gene and the peripheral atherosclerosis (atherosclerosis, AS) associated with type 2 diabetes in the Han nationality of Kunming. [Methods] the MT1A gene (rs8052394) and MT2A genes of the subjects were applied by the Taqman probe technique. A comparative analysis of type 2 diabetes with peripheral atherosclerosis (AS) (87 cases), simple T2DM group (T2DM) (170 cases) and healthy control group (NC) (108 cases), the correlation of genotype and allele frequency and related clinical and biochemical indexes. [results] (1) - like data and biochemical data in group T2DM and group AS Compared with group NC, Cr, UA, TG, FBG, BMI, HbA1C were higher in group AS and T2DM than in NC group, and HDL-C was lower than that in NC group. The difference between the other groups was not statistically significant. (2) the comparison between the 22 groups was compared with those in the other groups. ERG genetic equilibrium law (P0.05), A allele frequency 0.71, G allele frequency 0.29.AS group and T2DM group MT1A gene rs8052394 site A/A genotype frequency higher than NC group (X2=18.942, p0.001; The allele frequency of rs8052394 site A was higher than that of NC group (X2=17. 991, p0. 001; X2=27.823, p0.001), and there was no statistical difference between the AS group and the T2DM group (X2=3. 876, p=0. 049). (3) the polymorphism of the MT2A gene was compared with that of the group. RG genetic equilibrium law (P0.05), A allele frequency 0.76, G allele frequency 0. 24.T2DM group MT2A gene rs28366003 loci G/G genotype is higher than NC group (X2=16.601, p0.001), and the other groups have no statistical difference in genotype frequencies. 266, p0.001, X2=9. 082, p=0.003). (4) two classified Logistic regression analysis suggested that the rs28366003 polymorphism of MT2A gene may not be related to the occurrence of peripheral atherosclerosis in type 2 diabetes mellitus, HDL-C (OR value 43, 95%CI: 0. 10-0. 59) may be a protective factor for the occurrence of peripheral atherosclerosis in type 2 diabetes mellitus, MT1A gene The 94 A/A genotype may be an independent risk factor for the occurrence of peripheral atherosclerosis in type 2 diabetes. [Conclusion] the rs28366003 polymorphism of the MT2A gene in the Han population in Kunming region may not be related to the occurrence of peripheral atherosclerosis in type 2 diabetes mellitus, and the MT1A gene rs8052394 A/A genotype may be type 2 diabetes combined with peripheral blood. An independent risk factor for atherosclerosis.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R587.2

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