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  本文選題：膠原誘導關節(jié)炎 + 綠色熒光蛋白　； 參考：《山西醫(yī)科大學》2015年碩士論文

【摘要】：目的:GFP標記的BM-MSCs通過體外培養(yǎng)、擴增后移植到CIA大鼠體內(nèi),通過光學成像聯(lián)合免疫組化的方法檢測其在CIA大鼠免疫器官以及關節(jié)部位的遷移、定植和分布情況,探討其修復損傷的可能機制。方法:1.對GFP標記的MSCs進行體外培養(yǎng)、擴增及鑒定。2.將II型膠原與完全弗氏佐劑的混合制劑間隔14天兩次免疫Wistar大鼠,從大鼠癥狀(大體觀、關節(jié)炎指數(shù)、后肢腫脹程度)、影像學、病理學方面進行評估,建立CIA大鼠模型。隨機分為早期干預組(n=20)、晚期干預組(n=20),同時設置CIA早期對照組(n=12)、晚期對照組(n=12)和正常對照組(n=12)。3.尾靜脈途徑移植GFP-MSCs,小動物活體成像技術(shù)動態(tài)觀察移植MSCs在大鼠體內(nèi)的遷移過程。4.于3、11、30和42天將各組大鼠的脾臟、胸腺、淋巴結(jié)和關節(jié)組織作成病理切片,通過免疫組化的方法檢測移植細胞在免疫器官和炎癥關節(jié)部位的遷移和分布。結(jié)果:1.(1)早、晚期干預組關節(jié)炎指數(shù)、關節(jié)腫脹程度較CIA對照組明顯下降(P0.05);(2)早期干預組與晚期干預組比較,關節(jié)炎指數(shù)與關節(jié)腫脹程度降低,差異有統(tǒng)計學意義(P0.05)。2.小動物活體成像技術(shù)未能動態(tài)監(jiān)測到MSCs移植在CIA大鼠體內(nèi)的遷移過程,動物自身熒光干擾明顯。3.抗GFP免疫組化法在干預組大鼠免疫器官和關節(jié)部位成功檢測到GFP陽性結(jié)果,并可持續(xù)存在至少42天。結(jié)論:1.MSCs經(jīng)尾靜脈途徑移植后,可遷移至CIA大鼠脾臟、淋巴結(jié)和胸腺和關節(jié)炎癥部位,并可較長期(42天)定植于該組織。2.GFP不適用活體示蹤檢測MSCs在大鼠免疫器官及關節(jié)部位的遷移。3.MSCs干預對CIA大鼠有效,早期干預療效優(yōu)于晚期干預。
[Abstract]:Objective to investigate the migration, colonization and distribution of BM-MSCs labeled with GFP in the immune organs and joints of CIA rats by means of optical imaging combined with immunohistochemistry. To explore the possible mechanism of its repair injury. Method 1: 1. GFP labeled MSCs was cultured, amplified and identified in vitro. Wistar rats were immunized twice with collagen II and complete Freund adjuvant mixture for 14 days. The rat model of CIA was established by evaluating the symptoms (gross view, arthritis index, swelling degree of hind limbs, imaging and pathology). They were randomly divided into two groups: early intervention group, late intervention group, CIA early control group and late control group. GFP-MSCs was transplanted through the caudal vein and the migration process of transplanted MSCs in rats was observed dynamically by small animal in vivo imaging. The spleen, thymus, lymph nodes and joints of each group were made into pathological sections at 30 and 42 days after operation. The migration and distribution of transplanted cells in immune organs and inflammatory joints were detected by immunohistochemical method. Results (1) the arthritis index and joint swelling degree in the early and late intervention group were significantly lower than those in the CIA control group (P < 0.01). The arthritis index and the joint swelling degree in the early intervention group were significantly lower than those in the late intervention group, and the difference was statistically significant. In vivo imaging of small animals could not dynamically monitor the migration process of MSCs transplantation in CIA rats. The positive results of GFP were detected successfully in the immune organs and joints of the rats in the intervention group by immunohistochemical method of anti GFP, and the results were sustained for at least 42 days. Conclusion: 1. MSCs can migrate to spleen, lymph node, thymus and arthritis of CIA rats after transplantation via caudal vein. GFP could not be used to detect the migration of MSCs in the immune organs and joints of rats. 3. MSCs intervention was effective in CIA rats, and the early intervention effect was better than the late intervention.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R593.22;R457.7

【參考文獻】

相關期刊論文 前3條

1 王楠;張利;李清剛;胡婕;李典耕;師鎖柱;傅博;陳香美;;骨髓間充質(zhì)干細胞治療急性腹膜纖維化的作用方式探討[J];解放軍醫(yī)學雜志;2011年12期

2 王亭忠,馬愛群,蔣文慧,徐正云,席雨濤;大鼠骨髓間充質(zhì)干細胞的分離培養(yǎng)及GFP轉(zhuǎn)染標記[J];西安交通大學學報(醫(yī)學版);2005年05期

3 ;Advances in the application of quantum dots in tumor markers investigation[J];Chinese-German Journal of Clinical Oncology;2008年03期

相關碩士學位論文 前1條

1 孟紅光;大鼠骨髓間充質(zhì)干細胞（MSCs）移植在CIA大鼠中定植與分布的研究[D];山西醫(yī)科大學;2009年

，

本文編號：1896581