發(fā)布時間：2018-04-17 10:56

  本文選題：Orexin-A + PVN��； 參考：《青島大學》2015年碩士論文

【摘要】：目的:探討室旁核(PVN)微量注射orexin-A對大鼠體重的影響及機制。方法:采用PVN埋管、PVN微量注射,Echo MRI900體成分分析儀分析身體成分等方法,觀察室旁核微量注射orexin-A對大鼠體重、身體成分和自由活動的影響。結(jié)果:與注射人工腦脊液對照組相比,下丘腦PVN微量注射orexin-A,大鼠體重明顯減輕(P0.05),但大鼠攝食量無明顯差異(P㧐0.05)。實驗結(jié)束后6天,PVN微量注射orexin-A組大鼠體重仍顯著低于注射前(P0.05),而人工腦脊液對照組大鼠體重比注射前顯著增加(P0.05);與注射人工腦脊液對照組大鼠比較,PVN微量注射Orexin-A,大鼠肌肉組織、脂肪組織和非脂肪組織均顯著降低(P0.05),但注射orexin-A的大鼠降低更明顯。與注射人工腦脊液對照組相比,PVN微量注射orexin-A,大鼠的攝食轉(zhuǎn)化率(攝食量/體重)顯著降低(P0.05)。下丘腦PVN微量注射orexin-A,大鼠水平運動量顯著增加(P0.05)。結(jié)論:室旁核orexin-A可通過增加大鼠活動量而產(chǎn)生負能量平衡,體重減輕。
[Abstract]:Aim: to investigate the effect and mechanism of microinjection of PVN into paraventricular nucleus (PVN) on body weight of rats.Methods: the effects of microinjection of orexin-A into paraventricular nucleus on body weight, body composition and free activity of rats were observed by means of microinjection of Echo MRI900 body composition analyzer.Results: compared with the control group injected with artificial cerebrospinal fluid (ACSF), hypothalamic PVN microinjection of orexin-A significantly reduced the body weight of the rats, but there was no significant difference in the feeding intake of the rats.Six days after the experiment, the body weight of rats in the orexin-A group was still significantly lower than that in the pre-injection group, while the body weight of the rats in the artificial cerebrospinal fluid control group was significantly higher than that in the control group before injection, and compared with the control group of artificial cerebrospinal fluid injection of Orexin-A, the muscle tissue of the rats was significantly higher than that of the control group.Both adipose tissue and non-adipose tissue significantly decreased P0. 05%, but more significantly in rats injected with orexin-A.Compared with the control group injected with artificial cerebrospinal fluid (ACSF), the feed conversion rate (feed intake / body weight) of PVN was significantly lower than that of the control group treated with microinjection of orexin-A.After microinjection of orexin-A into hypothalamus PVN, the amount of horizontal exercise in rats increased significantly (P 0.05).Conclusion: paraventricular nucleus orexin-A can produce negative energy balance and weight loss by increasing the activity of rats.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R589.2

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