本文選題：克山病　切入點(diǎn)：生物標(biāo)志物　出處：《中國(guó)地方病防治雜志》2016年11期

【摘要】：目的通過檢測(cè)慢型克山病和對(duì)照組外周血中miR-320a、miR-18b、miR-22、Let-7b的表達(dá)水平,探討其作為疾病鑒別診斷的生物標(biāo)志物的意義。方法收集克山病病區(qū)慢型克山病患者(40例)、非病區(qū)對(duì)照組(40例)及病區(qū)對(duì)照組(40例)的血樣標(biāo)本,采用實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)各組研究對(duì)象血清中目標(biāo)miRNA的表達(dá)水平。結(jié)果 miR-320a、miR-18b和Let-7b在兩對(duì)照組間比較有統(tǒng)計(jì)學(xué)意義(P0.05);比較克山病組和病區(qū)對(duì)照組,miR-320a和Let-7b有統(tǒng)計(jì)學(xué)意義(P0.05),提示這3個(gè)目標(biāo)miRNA與高血壓具有相關(guān)性。miR-22在三組比較中均無意義。結(jié)論 miR-320a、miR-18b、miR-22和Let-7b在克山病患者中未見顯著表達(dá),為慢型克山病的鑒別診斷提供了新思路。
[Abstract]:Objective to detect the expression level of miR-320atir miR-18btimiR-22Let-7b in peripheral blood of patients with chronic Keshan disease and control group, and to explore its significance as a biomarker for differential diagnosis of the disease.Methods Blood samples were collected from 40 patients with chronic Keshan disease, 40 patients with chronic Keshan disease and 40 patients with chronic Keshan disease. The expression of target miRNA in serum of each group was detected by real-time fluorescence quantitative PCR.Results there was significant difference in miR-320aanmiR-18b and Let-7b between the two control groups, and the comparison of miR-320a and Let-7b in the Keshan disease group and the control group in the area of disease showed that the three target miRNA were correlated with hypertension. MiR-22 had no significance in the three groups.Conclusion there is no significant expression of miR-320a- miR-18btmiR-22 and Let-7b in patients with Keshan disease, which provides a new idea for differential diagnosis of chronic Keshan disease.
【作者單位】： 哈爾濱醫(yī)科大學(xué)中國(guó)疾病預(yù)防控制中心地方病控制中心克山病研究所;
【基金】：黑龍江省普通高校病因流行病學(xué)重點(diǎn)實(shí)驗(yàn)室評(píng)估優(yōu)秀獎(jiǎng)勵(lì)基金(2007400200007):克山病病區(qū)居民高血壓流行特征研究
【分類號(hào)】：R542.3
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本文編號(hào)：1720264