本文選題：Apelin　切入點(diǎn)：糖尿病腎病　出處：《中國(guó)醫(yī)院藥學(xué)雜志》2016年03期

【摘要】：目的:在高糖刺激的腎小球系膜細(xì)胞MES13和HBZY-1中研究Apelin-13抑制高糖引起的細(xì)胞炎癥。方法:在高糖誘導(dǎo)的MES13和HBZY-1細(xì)胞中給予不同劑量的Apelin-13,通過real time-PCR技術(shù)檢測(cè)各實(shí)驗(yàn)組2種系膜細(xì)胞中Icam-1和Mcp-1基因的mRNA水平變化。結(jié)果:(1)與空白對(duì)照組細(xì)胞相比,高糖組MES13和HBZY-1細(xì)胞中Icam-1和Mcp-1基因的mRNA水平顯著性上升(P0.05);給藥Apelin-13(300 pmol·L-1)能顯著性抑制高糖刺激的MES13和HBZY-1細(xì)胞中Icam-1和Mcp-1基因的mRNA水平(P0.05)。(2)與空白對(duì)照組細(xì)胞相比,通過免疫組化實(shí)驗(yàn)檢測(cè)高糖組MES13和HBZY-1細(xì)胞中促炎蛋白p38的磷酸化水平上升(P0.05);給藥Apelin-13(300 pmol·L-1)能顯著性抑制高糖引起MES13和HBZY-1細(xì)胞中促炎癥蛋白p38的磷酸化(P0.05)。結(jié)論:Apelin-13抑制高糖引起的MES13和HBZY-1細(xì)胞中p38的激活,從而抑制高糖所引起的炎癥反應(yīng)(Icam-1和Mcp-1基因水平)。
[Abstract]:Objective: To study the inhibition of Apelin-13 cells induced by high glucose in MES13 and HBZY-1 in mesangial cells stimulated by high glucose. The method given different dose in high glucose induced MES13 and HBZY-1 cells in Apelin-13, mRNA levels were detected by real time-PCR and Mcp-1 Icam-1 of each experimental group 2 mesangial cell genes. Results: (1) compared with the control group cells, Icam-1 and Mcp-1 genes of MES13 and HBZY-1 cells in high glucose group the level of mRNA was significantly increased (P0.05); administration of Apelin-13 (300 pmol L-1) Icam-1 and Mcp-1 can significantly inhibit glucose stimulated MES13 and HBZY-1 cells in gene level of mRNA (P0.05). (2) compared with the control group cells by immunohistochemical p38 assay of proinflammatory protein MES13 in high glucose group and HBZY-1 cells in the phosphorylation level increased (P0.05); administration of Apelin-13 (300 pmol L-1) could significantly inhibit high glucose induced The phosphorylation of proinflammatory protein p38 (P0.05) was observed in MES13 and HBZY-1 cells. Conclusion: Apelin-13 inhibits the activation of p38 in MES13 and HBZY-1 cells induced by high glucose, thereby inhibiting the inflammatory reaction induced by high glucose (Icam-1 and Mcp-1 gene level).

【作者單位】： 武漢市第三醫(yī)院;武漢沃爾藥業(yè)有限公司;
【基金】：武漢市衛(wèi)生計(jì)生委科研計(jì)劃資助項(xiàng)目(編號(hào):WX12B06) 湖北省自然科學(xué)基金面上項(xiàng)目(編號(hào):2013CFB359) 湖北衛(wèi)生和計(jì)劃生育委員會(huì)科研計(jì)劃資助項(xiàng)目(編號(hào):WJ2015MB147)
【分類號(hào)】：R587.2

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