本文關(guān)鍵詞： 絲膠 型糖尿病 肝臟 胰島素受體底物- 胰島素抵抗　出處：《中國(guó)老年學(xué)雜志》2016年19期 　論文類(lèi)型：期刊論文

【摘要】：目的研究絲膠對(duì)2型糖尿病大鼠肝臟胰島素受體底物(IRS)-2表達(dá)的影響。方法 36只雄性SD大鼠隨機(jī)分為正常對(duì)照組、糖尿病模型組、絲膠治療組,每組12只。采用鏈脲佐菌素連續(xù)腹腔注射法制作2型糖尿病大鼠模型。待模型成功建立后,絲膠治療組大鼠給予絲膠(2.4 g·kg~(-1)·d~(-1))灌胃。SP免疫組織化學(xué)染色、免疫印跡法和RT-PCR檢測(cè)大鼠肝臟組織IRS-2的表達(dá)。結(jié)果絲膠可明顯提高糖尿病模型大鼠肝臟組織IRS-2陽(yáng)性細(xì)胞數(shù)、IRS-2蛋白和mRNA的表達(dá)(P0.01)。結(jié)論絲膠可通過(guò)上調(diào)糖尿病大鼠肝臟IRS-2的表達(dá),影響胰島素的信號(hào)轉(zhuǎn)導(dǎo)和改善胰島素抵抗,起到降低血糖的作用。
[Abstract]:Objective to study the effect of sericin on the expression of insulin receptor substrate IRS-2 in liver of type 2 diabetic rats. Methods 36 male SD rats were randomly divided into normal control group, diabetic model group and sericin treatment group. The model of type 2 diabetic rats was established by continuous intraperitoneal injection of streptozotocin. After the successful establishment of the model, the rats in the sericin treatment group were given sericin 2.4 g 路kg ~ (-1) 路d ~ (-1)) by intragastric perfusion with immunohistochemical staining of SP. Results sericin could significantly increase the number of IRS-2 positive cells and the expression of IRS-2 protein and mRNA in the liver of diabetic rats. Conclusion sericin can up-regulate the expression of IRS-2 in the liver of diabetic rats. Affect insulin signal transduction and improve insulin resistance, play a role in reducing blood sugar.
【作者單位】： 承德醫(yī)學(xué)院附屬醫(yī)院檢驗(yàn)科;承德醫(yī)學(xué)院人體解剖學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81441133) 河北省自然科學(xué)基金項(xiàng)目(H2013406096) 河北省高校重點(diǎn)發(fā)展學(xué)科“人體解剖與組織胚胎學(xué)”建設(shè)項(xiàng)目資助(2013年)
【分類(lèi)號(hào)】：R587.1;R440
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本文編號(hào)：1547311