本文關(guān)鍵詞：c-junN端蛋白激酶1磷酸化與糖尿病大鼠早期心肌損傷關(guān)系研究　出處：《中國全科醫(yī)學》2016年27期 　論文類型：期刊論文

  更多相關(guān)文章： 糖尿病 心肌疾病 c-jun N端蛋白激酶 瞬時受體電位通道C亞族

【摘要】：目的探討c-jun N端蛋白激酶1(JNK1)磷酸化與糖尿病(DM)大鼠早期心肌損傷的關(guān)系。方法2013年10月—2014年7月,80只SPF級雌性SD大鼠適應性喂養(yǎng)1周后,采用隨機數(shù)字表法選取60只作為DM模型組,給予高糖高脂飲食飼養(yǎng)4周,一次性左下腹腹腔注射1%鏈脲佐菌素(STZ)溶液(35 mg/kg);另外20只作為對照組(A組),給予普通飲食飼養(yǎng)4周,一次性左下腹腹腔注射等量枸櫞酸緩沖液。最終共48只大鼠造模成功,采用隨機數(shù)字表法分為B組、C組、D組,各16只。C組大鼠腹腔注射SP600125,D組大鼠皮下埋置含有血管緊張素Ⅱ(AngⅡ)+0.9%氯化鈉溶液的植入式膠囊滲透壓泵,A組、B組大鼠腹腔注射0.9%氯化鈉溶液。飼養(yǎng)6周后,采用隨機數(shù)字表法從4組大鼠中分別選取10只,檢測大鼠心體比(H/B)、左心室質(zhì)量指數(shù)(LVMI),HE染色觀察心肌組織形態(tài)學改變,免疫組化SABC法檢測JNK1、磷酸化JNK1(p-JNK1)、瞬時受體電位通道C亞族(TRPC)6、膠原蛋白Ⅰ(collagenⅠ)表達水平。結(jié)果 B組、C組、D組大鼠H/B大于A組,B組、D組大鼠LVMI大于A組(P0.05);C組大鼠H/B、LVMI小于B組、D組(P0.05)。A組大鼠心肌細胞排列整齊,細胞間連接緊密,心肌紋理清晰;B組、C組、D組大鼠心肌細胞均有不同程度的肥大性改變,細胞間隙增寬,心肌細胞排列紊亂,心肌紋理欠清晰。B組、C組、D組大鼠JNK1、p-JNK1、TRPC6、collagenⅠ表達水平高于A組(P0.05);C組大鼠JNK1、p-JNK1、TRPC6、collagenⅠ表達水平低于B組(P0.05);D組大鼠p-JNK1、TRPC6、collagenⅠ表達水平高于B組、C組,JNK1表達水平高于C組(P0.05)。結(jié)論高糖環(huán)境可誘導JNK1磷酸化,進而影響下游TRPC6信號表達,造成膠原蛋白沉積過多,導致心肌細胞肥大和纖維化,從而引起糖尿病心肌病的發(fā)生發(fā)展。
[Abstract]:Objective to investigate the relationship between the phosphorylation of c-jun N-terminal protein kinase 1 (JNK1) and early myocardial injury in rats with diabetes mellitus (DM). Methods from October 2013 to July 2014. After one week of adaptive feeding, 60 SPF female SD rats were selected as DM model group and fed with high sugar and high fat diet for 4 weeks. 1% mg / kg of streptozotocin (STZ) solution was injected intraperitoneally into the left lower abdomen. The other 20 rats were fed with general diet for 4 weeks, and the same amount of citric acid buffer was injected into the abdominal cavity of left abdomen. Finally, 48 rats were successfully established. The rats in group B were randomly divided into group C (n = 16) and group C (n = 16). SP600125 was injected intraperitoneally in each group (n = 16). Rats in group D were subcutaneously implanted with angiotensin 鈪，

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