【摘要】：目的:通過(guò)觀察薯蕷多糖體外對(duì)精子膜及DNA完整性的影響。為少弱精子癥的治療提供新的途徑。方法:正常已生育成年男性,禁欲3~5 d手淫法取精液。分為6組,每組各取0.2 ml精液;對(duì)照組為精液原液,其他5組分別加入等量的生理鹽水、維生素C溶液及不同濃度薯蕷多糖溶液(0.25、1.0、5.0 mg/ml)。通過(guò)伊紅-Y染色試驗(yàn)、精子低滲腫脹實(shí)驗(yàn)(HOS)以及精子染色質(zhì)擴(kuò)散(SCD)實(shí)驗(yàn),觀察不同濃度薯蕷多糖體外對(duì)精子存活率、精子膜完整性以及精子核DNA的影響。結(jié)果:6份精液樣本孵育24 h及48 h后各組的精子存活率均有降低。維生素C組精子存活率分別為(28.5±3.1)%、(6.5±1.2)%;薯蕷多糖低、中、高劑量組的精子存活率分別為(31.3±3.5)%、(6.5±2.2)%,(37.1±3.5)%、(9.5±2.8)%,(38.3±3.3)%、(9.0±3.2)%,均顯著高于生理鹽水組[(13.4±4.1)%、(3.1±2.0)%,P0.01],中、高劑量薯蕷多糖組顯著高于維生素C組(P0.05及P0.01)。精子DNA碎片率低劑量薯蕷多糖組為(29.4±2.6)%,與維生素C組[(30.5±2.5)%]無(wú)明顯差異,但顯著低于對(duì)照組[(41.7±2.2)%]和生理鹽水組[(42.1±3.3)%];中、高劑量薯蕷多糖組精子DNA碎片率分別為(28.5±2.3)%和(27.9±1.9)%,顯著低于對(duì)照組、生理鹽水組及維生素C組(P0.05及P0.01)。結(jié)論:薯蕷多糖具有體外提高精子存活率和保護(hù)精子DNA完整性的作用。
[Abstract]:Objective: to observe the effect of Dioscorea zingiberensis polysaccharide on sperm membrane and DNA integrity in vitro. It provides a new way for the treatment of oligoasthenospermia. Methods: normal fertile adult male, abstinence 3 days 5 days by hand to get semen. Six groups were divided into 6 groups, each group received 0.2 ml semen, the control group was semen fluid, the other 5 groups were added the same amount of saline, vitamin C solution and different concentration of dioscorea solution (0.25, 1.0, 5.0 mg/ml). The effects of Dioscorea zingiberensis on sperm viability, sperm membrane integrity and sperm nuclear DNA were observed in vitro by eosin-Y staining, sperm hypoosmotic swelling test (HOS) and sperm chromatin diffusion (SCD) test. Results: the sperm survival rate of each group decreased after 24 h and 48 h incubation with 6 semen samples. The sperm survival rate in vitamin C group was (28.5 鹵3.1)% and (6.5 鹵1.2)%, respectively. The sperm survival rates in the high dose group were (31.3 鹵3.5)%, (6.5 鹵2.2)%, (37.1 鹵3.5)%, (9.5 鹵2.8)%, (38.3 鹵3.3)%, (9.0 鹵3.2)%, respectively. It was significantly higher than that in saline group [(13.4 鹵4.1)%, (3.1 鹵2.0)%, P0.01], and that in high-dose dioscorea group was significantly higher than that in vitamin C group (P0.05 and P0.01). The sperm DNA fragmentation rate was (29.4 鹵2.6)% in the low-dose dioscorea group, which was not significantly different from that in the vitamin C group [(30.5 鹵2.5)%]. But it was significantly lower than that of the control group [(41.7 鹵2.2)%] and the saline group [(42.1 鹵3.3)%]. The DNA fragmentation rate of high dose dioscorea polysaccharide group was (28.5 鹵2.3)% and (27.9 鹵1.9)% respectively, which was significantly lower than that of control group, physiological saline group and vitamin C group (P0.05 and P0.01). Conclusion: Dioscorea zingiberensis can improve sperm survival rate and protect sperm DNA integrity in vitro.
【作者單位】： 山東省婦幼保健院遺傳中心國(guó)家衛(wèi)生和計(jì)劃生育委員會(huì)生育調(diào)控技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：山東省科技發(fā)展計(jì)劃(2015GSF118072)~~
【分類號(hào)】：R698.2

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