【摘要】：目的探討膀胱癌細胞中著色性干皮病C組基因(xeroderma pigmentosum group C,XPC)蛋白缺失對自噬的影響。方法利用shRNA策略,建立穩(wěn)定干擾XPC的膀胱癌T24細胞模型,蛋白印跡技術(shù)檢測干擾XPC后自噬表征蛋白LC3Ⅱ的表達,瞬時轉(zhuǎn)染GFP-LC3,熒光顯微鏡觀察細胞熒光聚集情況,CCK-8法檢測細胞的增殖狀況,免疫印跡技術(shù)檢測自噬及凋亡相關(guān)蛋白的表達。結(jié)果成功建立干擾XPC的膀胱癌T24細胞模型;干擾XPC后,膀胱癌T24細胞自噬小體的形成[(1.53±0.81)%vs(3.30±0.70)%,P0.05]明顯減少,順鉑作用下膀胱癌T24細胞自噬小體的形成[(2.19±0.37)%vs(3.20±0.29)%,P0.05]明顯減少;順鉑促進XPC缺失后的膀胱癌T24細胞自噬;XPC缺失后的自噬為非保護性自噬。結(jié)論 XPC蛋白參與調(diào)控膀胱癌T24細胞的自噬。
[Abstract]:Objective to investigate the effect of deletion of C gene (xeroderma pigmentosum group CX PC on autophagy in bladder cancer cells. Methods the T24 cell model of bladder cancer which interfered with XPC was established by using shRNA strategy. The expression of autophagy protein LC3 鈪，

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