【摘要】：目的采用微計(jì)算機(jī)斷層掃描技術(shù)(Mirco-CT)和病理組織學(xué)檢查動(dòng)態(tài)觀察納米細(xì)菌誘導(dǎo)的大鼠腎結(jié)石模型腎臟結(jié)石形成情況。方法 2015年11月—2016年6月,將60只SPF級(jí)Wistar大鼠適應(yīng)性飼養(yǎng)1周后,隨機(jī)分為對(duì)照組和誘石組,各30只。對(duì)照組大鼠一次性尾靜脈注射0.9%氯化鈉溶液1.2 ml,誘石組大鼠一次性尾靜脈注射納米細(xì)菌懸液1.2 ml。兩組大鼠注射后第1~10周每周處死3只,收集雙側(cè)腎臟待測(cè)。采用Mirco-CT檢查兩組大鼠腎臟,統(tǒng)計(jì)第1~10周兩組大鼠檢測(cè)到的腎臟高密度影情況。光學(xué)顯微鏡下觀察兩組大鼠腎臟晶體的形成情況,統(tǒng)計(jì)第1~10周兩組大鼠腎臟晶體陽(yáng)性情況。結(jié)果 Mirco-CT掃描結(jié)果:第1~10周,對(duì)照組大鼠腎臟均未見(jiàn)高密度影;第7~10周誘石組大鼠腎臟可見(jiàn)白色顆粒樣高密度影。第1~10周,誘石組大鼠腎臟高密度影檢出4只(13.3%),對(duì)照組未檢出;截至第10周末,兩組大鼠腎臟高密度影檢出率比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.056)。病理組織學(xué)檢查結(jié)果:第1~10周,對(duì)照組大鼠腎臟內(nèi)無(wú)晶體沉積。第4周及第6~10周誘石組大鼠腎臟可見(jiàn)灰白色晶體。第1~10周,誘石組大鼠腎臟晶體陽(yáng)性11只(36.7%),對(duì)照組均為陰性;截至第10周末,誘石組大鼠腎臟晶體陽(yáng)性率高于對(duì)照組(P0.001)。結(jié)論納米細(xì)菌在第4周開(kāi)始誘導(dǎo)大鼠腎臟形成結(jié)晶,這個(gè)病理過(guò)程在第6~10周持續(xù)存在,Mirco-CT檢查可見(jiàn)第7~10周形成高密度結(jié)石樣物質(zhì),納米細(xì)菌可能通過(guò)早期損傷腎臟誘導(dǎo)結(jié)晶形成并進(jìn)一步產(chǎn)生結(jié)石。
[Abstract]:Objective to observe the formation of renal calculi in rat renal calculi induced by nanobacteria by microcomputed tomography (Mirco-CT) and histopathological examination. Methods from November 2015 to June 2016, 60 SPF grade Wistar rats were fed adaptively for one week, and were randomly divided into two groups: control group and induced stone group, 30 rats in each group. Control group rats were injected with 0.9% sodium chloride solution 1.2 ml, once a tail vein injection of nano-bacteria suspension 1.2 ml. Three rats were killed 10 weeks after injection, and bilateral kidneys were collected to be tested. Mirco-CT was used to examine the kidney of the two groups. The formation of renal lens in the two groups was observed under optical microscope, and the positive rate of renal lens in the first 10 weeks of the two groups was counted. Results the results of Mirco-CT: at the 1st week, there were no high density shadows in the kidneys of the control group, and white granulocylike high density shadows were observed in the kidneys of the rats in the induced lithotripsy group at the 7th week. At the 1st week, 4 rats (13.3%) were detected by renal hyperdensitometry in the induced lithotripsy group, but not by the control group, but there was no significant difference between the two groups at the end of the 10th week (P0. 056). Results of histopathological examination: at the 1st week, there was no crystal deposition in the kidney of the control group. Grayish white crystals could be seen in the kidney of the rats in the 4 th and 6 th week induced lithotripsy group. At the first week of 10 weeks, 11 rats (36.7%) were positive for renal lens in the induced lithotripsy group, and all the rats in the control group were negative, and at the end of the 10th week, the positive rate of the kidney lens in the induced lithotripsy group was higher than that in the control group (P0.001). Conclusion Nano bacteria began to induce the formation of crystals in the kidney of rats at the 4th week. This pathological process persisted at 6 and 10 weeks, and high density lithium-like substance was found on Mirco-CT examination at 7 ~ 10 weeks. Nano-bacteria may induce crystallization through early kidney injury and further produce stones.
【作者單位】： 石河子大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院泌尿外科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81460140)
【分類號(hào)】：R-332;R692.4

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