包皮彈性的臨床研究
發(fā)布時間：2018-10-25 14:44
【摘要】：【目的】 通過對包皮彈性的研究，將其結(jié)果應(yīng)用在包皮作修復(fù)材料的手術(shù)中，指導(dǎo)合理取材，，用材，從而提高手術(shù)的成功率。 【方法】 將2012年12月至2013年6月在郴州市第一人民醫(yī)院泌尿科行包皮環(huán)切手術(shù)7~30歲病人100例。按年齡分組：7~14y：兒童組（A組36例）；15~18y：青年組（B組32例）；19~30y：成人組（C組32例）。分別從已切下的包皮的內(nèi)外板上的不同部位（腹側(cè)、背側(cè)、旁側(cè)）取材，依次在不同的方向（橫向、縱向）牽拉，測量包皮的伸展度。測量完后使用10%的福爾馬林固定保存，制成病理切片。應(yīng)用免疫組化及彈力纖維染色來檢測各包皮切片的彈性纖維、平滑肌含量，并比較各組內(nèi)間差異。 【結(jié)果】 1.分析同組內(nèi)包皮的內(nèi)外板上的相同部位在相同牽拉方向下有無彈性差異。對A、B、 C組內(nèi)分別進行t檢驗，P值均0.05,不具有統(tǒng)計學(xué)意義，認(rèn)為同組內(nèi)包皮的內(nèi)外板上的相同部位在相同牽拉方向下彈性無顯著性差異。 2.分析同組內(nèi)相同內(nèi)外板上相同部位的包皮在不同牽拉方向下彈性有無差異。對A、B、 C組內(nèi)分別t檢驗結(jié)果，P值均0.05，具有統(tǒng)計學(xué)意義，根據(jù)均值大小，可認(rèn)為同組內(nèi)包皮的相同部位在橫向牽拉上的彈性要好于縱向。 3.分析同組內(nèi)相同內(nèi)外板上不同部位的包皮在相同牽拉方向上彈性有無差異。對A、B、C三組分別進行方差分析，P值均0.05，不具有統(tǒng)計學(xué)意義，認(rèn)為在同一年齡組內(nèi)相同內(nèi)外板上不同部位的包皮在相同牽拉方向上彈性無顯著性差異。 4.分析不同組間包皮彈性有無差異，即：年齡與包皮彈性有無關(guān)系。A組與B組，B組與C組，A組與C組間彈性兩兩進行t檢驗，P值均0.05，不具有統(tǒng)計學(xué)意義，無顯著性差異，認(rèn)為年齡與包皮彈性大小無關(guān)。 5.分析包皮的厚度與彈性有無關(guān)系。對包皮厚度和彈性數(shù)據(jù)進行Pearson相關(guān)分析，r=0.906，在0.01水平（雙側(cè)）上顯著相關(guān)。認(rèn)為，包皮的彈性與厚度之間具有直線負(fù)相關(guān)關(guān)系。 6.分析各年齡組間包皮平滑肌含量有無差異。A組結(jié)蛋白表達程度4.72±0.66，B組結(jié)蛋白表達程度4.07±0.55，C組結(jié)蛋白表達程度3.01±0.82。對A、B、C三組進行單因素方差分析，兩兩比較采用LSD-t檢驗，P值0.05，具有統(tǒng)計學(xué)意義，認(rèn)為A、B、C組結(jié)蛋白含量差異具有顯著性。根據(jù)均值大小，可認(rèn)為包皮組織標(biāo)本上的結(jié)蛋白含量（即平滑肌含量）隨著年齡增長逐漸減少。 7.分析各年齡組間包皮彈性纖維含量有無差異。對A、B、C三組進行LSD-t檢驗，P值0.05，具有統(tǒng)計學(xué)意義，認(rèn)為A、B、C組彈性纖維含量差異具有顯著性。根據(jù)均值大小，可認(rèn)為包皮組織標(biāo)本上的彈性纖維含量隨著年齡增長逐漸增多。 【結(jié)論】 1.包皮在橫向的牽拉長度大于縱向。 2.包皮組織越厚其彈性越差，反之亦然。 3.包皮組織標(biāo)本上的平滑肌含量隨著年齡增長逐漸減少。 4.包皮組織標(biāo)本上的彈性纖維含量隨著年齡增長逐漸增多。
[Abstract]:[objective] to study the elasticity of the prepuce and apply the results to the operation of the prepuce as the repair material to guide the reasonable selection and use of the material. [methods] from December 2012 to June 2013, 100 patients aged from 7 to 30 years old underwent circumcision of prepuce in Department of urology, the first people's Hospital of Chenzhou. According to the age group, children group (group A, n = 36), young group (group B, n = 32) and adult group (n = 32, n = 32), group A (n = 36), group B (n = 32), group B (n = 32). The different parts (ventral side, dorsal side, side) of the outer and outer plates of the cut prepuce were taken, and the extensibility of the prepuce was measured in different directions (transverse and longitudinal). After the measurement, 10% formalin was fixed and preserved to make pathological sections. The content of elastic fiber and smooth muscle of each prepuce section was detected by immunohistochemistry and elastic fiber staining, and the difference between each group was compared. [results] 1. To analyze the elastic difference of the same part on the inner and outer plate of the same group in the same pulling direction. T test was carried out in group A B and group C, P values were 0.05, there was no statistical significance. It was concluded that there was no significant difference in elasticity between the same part of the inner and outer plates of the same group under the same pulling direction. 2. The elasticity of the same part of prepuce on the same internal and external plate in the same group was analyzed under different pulling directions. According to the mean value, the elasticity of the same part of the prepuce in the same group was better than that in the longitudinal. The elasticity of different parts of the same internal and external plate in the same group in the same stretch direction was analyzed. The results of ANOVA showed that there was no significant difference in the elasticity of different parts of the prepuce on the same internal and external plate in the same age group in the same pulling direction. 4. There was no significant difference between group A and group B, group B and group C, group A and group C were tested by t test (P = 0.05), there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C, and there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C by t test. It is considered that age has nothing to do with the size of prepuce elasticity. 5. The relationship between the thickness of prepuce and elasticity is analyzed. The Pearson correlation analysis of prepuce thickness and elastic data showed that rn was 0.906, which was significantly correlated at 0. 01 level (bilateral). It is concluded that there is a linear negative correlation between the elasticity and thickness of the foreskin. The expression degree of desmin in group A was 4.72 鹵0.66mb and the expression level of desmin was 3.01 鹵0.82in group C (4.07 鹵0.55C). The single factor ANOVA was used in the three groups, and the LSD-t test was used to compare the two groups (P = 0.05). The results showed that there was a significant difference in the content of desmin in group A and B (P = 0.05), and the results showed that there was a significant difference in the content of desmin between the two groups. According to the mean value, the desmin content (that is, smooth muscle content) in prepuce tissue decreased gradually with age. The content of prepuce elastic fiber in different age groups was analyzed. The results of LSD-t test showed that there was a significant difference in elastic fiber content among three groups (P = 0.05). According to the mean value, it can be concluded that the content of elastic fiber in prepuce tissue increases with age. [conclusion] 1. The length of the circumference in the transverse stretch is larger than that in the longitudinal. 2. The thicker the foreskin is, the less elastic it is, and vice versa. The content of smooth muscle in prepuce tissue decreased with age. 4. 4. The content of elastic fibers in prepuce tissue increased with age.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R699.8
本文編號：2293998
[Abstract]:[objective] to study the elasticity of the prepuce and apply the results to the operation of the prepuce as the repair material to guide the reasonable selection and use of the material. [methods] from December 2012 to June 2013, 100 patients aged from 7 to 30 years old underwent circumcision of prepuce in Department of urology, the first people's Hospital of Chenzhou. According to the age group, children group (group A, n = 36), young group (group B, n = 32) and adult group (n = 32, n = 32), group A (n = 36), group B (n = 32), group B (n = 32). The different parts (ventral side, dorsal side, side) of the outer and outer plates of the cut prepuce were taken, and the extensibility of the prepuce was measured in different directions (transverse and longitudinal). After the measurement, 10% formalin was fixed and preserved to make pathological sections. The content of elastic fiber and smooth muscle of each prepuce section was detected by immunohistochemistry and elastic fiber staining, and the difference between each group was compared. [results] 1. To analyze the elastic difference of the same part on the inner and outer plate of the same group in the same pulling direction. T test was carried out in group A B and group C, P values were 0.05, there was no statistical significance. It was concluded that there was no significant difference in elasticity between the same part of the inner and outer plates of the same group under the same pulling direction. 2. The elasticity of the same part of prepuce on the same internal and external plate in the same group was analyzed under different pulling directions. According to the mean value, the elasticity of the same part of the prepuce in the same group was better than that in the longitudinal. The elasticity of different parts of the same internal and external plate in the same group in the same stretch direction was analyzed. The results of ANOVA showed that there was no significant difference in the elasticity of different parts of the prepuce on the same internal and external plate in the same age group in the same pulling direction. 4. There was no significant difference between group A and group B, group B and group C, group A and group C were tested by t test (P = 0.05), there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C, and there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C by t test. It is considered that age has nothing to do with the size of prepuce elasticity. 5. The relationship between the thickness of prepuce and elasticity is analyzed. The Pearson correlation analysis of prepuce thickness and elastic data showed that rn was 0.906, which was significantly correlated at 0. 01 level (bilateral). It is concluded that there is a linear negative correlation between the elasticity and thickness of the foreskin. The expression degree of desmin in group A was 4.72 鹵0.66mb and the expression level of desmin was 3.01 鹵0.82in group C (4.07 鹵0.55C). The single factor ANOVA was used in the three groups, and the LSD-t test was used to compare the two groups (P = 0.05). The results showed that there was a significant difference in the content of desmin in group A and B (P = 0.05), and the results showed that there was a significant difference in the content of desmin between the two groups. According to the mean value, the desmin content (that is, smooth muscle content) in prepuce tissue decreased gradually with age. The content of prepuce elastic fiber in different age groups was analyzed. The results of LSD-t test showed that there was a significant difference in elastic fiber content among three groups (P = 0.05). According to the mean value, it can be concluded that the content of elastic fiber in prepuce tissue increases with age. [conclusion] 1. The length of the circumference in the transverse stretch is larger than that in the longitudinal. 2. The thicker the foreskin is, the less elastic it is, and vice versa. The content of smooth muscle in prepuce tissue decreased with age. 4. 4. The content of elastic fibers in prepuce tissue increased with age.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R699.8
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相關(guān)期刊論文 前3條
1 余靈祥,鄒仲之;主動脈平滑肌細胞在三維培養(yǎng)中產(chǎn)生彈性纖維的研究[J];解剖學(xué)報;2004年01期
2 裴憲武;包皮為供區(qū)的皮膚移植[J];中國修復(fù)重建外科雜志;2003年02期
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本文編號：2293998
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