【摘要】：目的：探討膀胱癌患者外周血與正常人外周血樹突狀細胞(dendritic cells,DCs)免疫功能的差異以及B7-HI阻斷對膀胱癌患者外周血DCs免疫功能的影響。 方法：采集膀胱癌患者及正常人的外周血,用rhGM-CSF、rhIL-4和rhTNF-a自外周血單個核細胞(peripheral blood mononuclear cell, PBMC)誘導分離DCs,混合淋巴細胞反應檢測DCs刺激淋巴細胞增殖的能力。然后于膀胱癌DCs組加入B7-H1的阻斷型抗體,混合淋巴細胞反應檢測B7-H1阻斷后DCs刺激淋巴細胞增殖的能力,ELISA法檢測阻斷后DCs分泌IL-10和IL-12的水平。 結果：膀胱癌患者外周血DCs刺激淋巴細胞增殖的能力低于正常人外周血DCs,而加入B7-H1阻斷型抗體阻斷B7-H1后,膀胱癌患者外周血DCs刺激淋巴細胞增殖的能力得到明顯提高,同時其分泌IL-I0水平明顯降低,而分泌IL-12水平明顯增加。 結論：膀胱癌患者外周血DCs存在免疫功能缺陷,其機制可能與DCs表面B7-H1的誘導高表達有關,而B7-H1阻斷能夠增強膀胱癌患者外周血DCs的免疫功能。
[Abstract]:Aim: to investigate the difference of dendritic cell (dendritic cells,DCs) immune function between peripheral blood of bladder cancer patients and normal controls and the effect of B7-HI blockade on DCs immune function in peripheral blood of bladder cancer patients. Methods: peripheral blood samples were collected from patients with bladder cancer and normal controls. The ability of DCs stimulated lymphocyte proliferation was detected by DCs, mixed lymphocyte reaction induced by rhGM-CSF,rhIL-4 and rhTNF-a from peripheral blood mononuclear cells (PBMC). Then the blocking antibody of B7-H1 was added to the DCs group of bladder cancer. The ability of DCs to stimulate the proliferation of lymphocytes after B7-H1 was detected by mixed lymphocyte reaction. The levels of IL-10 and IL-12 secreted by DCs were detected by ELISA assay. Results: the ability of peripheral blood DCs to stimulate lymphocyte proliferation in patients with bladder cancer was lower than that in normal peripheral blood DCs, but the ability of peripheral blood DCs to stimulate lymphocyte proliferation was significantly improved after B7-H1 blocking antibody was added to block B7-H1. At the same time, the level of secretory IL-I0 decreased significantly, but the level of secretory IL-12 increased significantly. Conclusion: DCs in peripheral blood of bladder cancer patients has immune function defect and its mechanism may be related to the overexpression of B7-H1 on the surface of DCs. B7-H1 blocking can enhance the immune function of DCs in peripheral blood of bladder cancer patients.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.14

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