【摘要】：目的建立腎缺血再灌注損傷(ischemia-reperfusion injury, TRI)缺血后處理(ischemic postconditioning, IPO)大鼠模型,研究IPO對缺血再灌住損傷腎基因表達譜的影響,探討其保護機制。 方法選用8-12周成年健康SD雄性大鼠60只,隨機分為6組：對照組(S組)、缺血再灌注組IR缺血后處理A、B、C、D組,通過測定血尿素氮(BUN)和肌酐(SCr),腎組織結(jié)構(gòu)損傷程度評分,篩選出成功的IPO模型。然后選取成年健康SD雄性大鼠6只分為2組：缺血再灌注組和IPO組。缺血再灌注組：結(jié)扎右側(cè)腎蒂,夾閉左側(cè)腎蒂60min后恢復(fù)灌注。IPO組：在腎臟缺血60min后進行6次開放10s+阻斷10s的循環(huán)然后充分開放灌注。飼養(yǎng)24h后迅速切取左腎,分別將左腎組織放入液氮低溫保存。進行RNA抽提、探針制備和芯片雜交,雜交后立即進行掃描檢測信號收集圖像。應(yīng)用Softfire Decisionsite8.0軟件分析某基因是否有表達,以及兩組表達程度是否有差異。根據(jù)基因編碼蛋白的功能對差異表達超過兩倍的基因進行初步分析。 結(jié)果缺血后處理組與缺血再灌注組腎臟組織比較共有70條基因表達有差異,基因表達上調(diào)的有48條,基因表達下調(diào)的基因有22條。這些基因主要涉及細胞代謝與損傷、凋亡調(diào)控相關(guān)蛋白、信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄、炎癥相關(guān)因子、氧化應(yīng)激相關(guān)因子等。 結(jié)論成功建立了腎缺血再灌注損傷IPO大鼠模型,IPO可能作用于多個基因靶點,減輕腎臟缺血再再灌注損傷。
[Abstract]:Objective to establish a model of renal ischemia-reperfusion injury (ischemia-reperfusion injury, TRI) in (ischemic postconditioning, IPO) rats, to study the effect of IPO on renal gene expression profile of ischemia-reperfusion injury and to explore its protective mechanism. Methods Sixty male adult SD rats aged 8-12 weeks were randomly divided into 6 groups: control group (S group) and ischemic reperfusion group (group D) treated with IR after ischemia. Blood urea nitrogen (BUN) and creatinine (SCr), (SCr),) were measured. The successful IPO model was screened out. Then 6 male adult SD rats were divided into two groups: ischemia reperfusion group and IPO group. In the ischemia-reperfusion group, the right renal pedicle was ligated, the left renal pedicle 60min was clipped and the perfusion was restored. In the IPO group, the circulation was blocked for 10 s for 6 times and then the perfusion was fully opened after 10 s of renal ischemia 60min. After feeding for 24 hours, the left kidney was quickly removed and stored in liquid nitrogen. RNA extraction, probe preparation and chip hybridization were performed immediately after hybridization. Softfire Decisionsite8.0 software was used to analyze whether a gene was expressed and whether there were differences between the two groups. According to the function of gene encoding protein, the differentially expressed genes were preliminarily analyzed. Results there were different expression of 70 genes in renal tissues between the post-ischemic group and the ischemia-reperfusion group. There were 48 up-regulated genes and 22 down-regulated genes. These genes are mainly involved in cell metabolism and injury, apoptosis-related proteins, signal transduction and transcription, inflammatory related factors, oxidative stress related factors and so on. Conclusion IPO rat model of renal ischemia-reperfusion injury was successfully established. IPO may act on multiple gene targets to alleviate renal ischemia-reperfusion injury.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R692

【參考文獻】

相關(guān)期刊論文 前10條

1 唐鐵龍;盧一平;周棱;余志海;靳偉;;大鼠腎臟熱缺血再灌注損傷的缺血后處理模型的建立[J];華西醫(yī)學(xué);2006年03期

2 王忠;翁國星;杜建;;川芎嗪預(yù)處理急性缺血性腎衰竭基因譜研究[J];中國中西醫(yī)結(jié)合腎病雜志;2006年04期

3 呂青蘭,袁燦,張華莉,陳廣文,王堯玲,鄧恭華,涂自智,肖獻忠;用cDNA芯片檢測大鼠心肌缺血預(yù)適應(yīng)后基因表達譜的改變[J];中國動脈硬化雜志;2003年03期

4 付慧;董文斌;;核因子-κB與腎臟的缺血再灌注損傷[J];中國婦幼健康研究;2006年06期

5 王莉娜;陸燕蓉;程驚秋;;基因芯片技術(shù)在移植器官缺血再灌注損傷研究中的應(yīng)用[J];生物醫(yī)學(xué)工程學(xué)雜志;2006年05期

6 李文瀾;柯偉;胡剛;劉先義;;大鼠腎缺血后處理血清一氧化氮及一氧化氮合酶的變化[J];實用醫(yī)學(xué)雜志;2008年10期

7 李榮山;丁濤;劉曉城;李彩霞;;Influence of SB203580 on Cell Apoptosis and P38MAPK in Renal Ischemia/Reperfusion Injury[J];華中科技大學(xué)學(xué)報(醫(yī)學(xué)英德文版);2006年01期

8 王楠,馬慶久,褚延魁,何顯力,杜錫林;缺血后處理對異品系大鼠移植肝臟的保護作用[J];中國臨床康復(fù);2005年18期

9 高峰,YanWL,GengYJ,朱妙章,臧益民,馬新亮,蔡振杰;“缺氧后處理”對大鼠缺氧-復(fù)氧心室肌細胞的保護作用[J];心功能雜志;1999年04期

10 石磊;蔡尚郎;劉松;張文忠;;RISK信號轉(zhuǎn)導(dǎo)通路與腎缺血后處理的心肌保護作用[J];中國心血管雜志;2007年01期

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