【摘要】：目的建立腎缺血再灌注損傷(ischemia-reperfusion injury, TRI)缺血后處理(ischemic postconditioning, IPO)大鼠模型,研究IPO對(duì)缺血再灌住損傷腎基因表達(dá)譜的影響,探討其保護(hù)機(jī)制。 方法選用8-12周成年健康SD雄性大鼠60只,隨機(jī)分為6組：對(duì)照組(S組)、缺血再灌注組IR缺血后處理A、B、C、D組,通過(guò)測(cè)定血尿素氮(BUN)和肌酐(SCr),腎組織結(jié)構(gòu)損傷程度評(píng)分,篩選出成功的IPO模型。然后選取成年健康SD雄性大鼠6只分為2組：缺血再灌注組和IPO組。缺血再灌注組：結(jié)扎右側(cè)腎蒂,夾閉左側(cè)腎蒂60min后恢復(fù)灌注。IPO組：在腎臟缺血60min后進(jìn)行6次開(kāi)放10s+阻斷10s的循環(huán)然后充分開(kāi)放灌注。飼養(yǎng)24h后迅速切取左腎,分別將左腎組織放入液氮低溫保存。進(jìn)行RNA抽提、探針制備和芯片雜交,雜交后立即進(jìn)行掃描檢測(cè)信號(hào)收集圖像。應(yīng)用Softfire Decisionsite8.0軟件分析某基因是否有表達(dá),以及兩組表達(dá)程度是否有差異。根據(jù)基因編碼蛋白的功能對(duì)差異表達(dá)超過(guò)兩倍的基因進(jìn)行初步分析。 結(jié)果缺血后處理組與缺血再灌注組腎臟組織比較共有70條基因表達(dá)有差異,基因表達(dá)上調(diào)的有48條,基因表達(dá)下調(diào)的基因有22條。這些基因主要涉及細(xì)胞代謝與損傷、凋亡調(diào)控相關(guān)蛋白、信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄、炎癥相關(guān)因子、氧化應(yīng)激相關(guān)因子等。 結(jié)論成功建立了腎缺血再灌注損傷IPO大鼠模型,IPO可能作用于多個(gè)基因靶點(diǎn),減輕腎臟缺血再再灌注損傷。
[Abstract]:Objective to establish a model of renal ischemia-reperfusion injury (ischemia-reperfusion injury, TRI) in (ischemic postconditioning, IPO) rats, to study the effect of IPO on renal gene expression profile of ischemia-reperfusion injury and to explore its protective mechanism. Methods Sixty male adult SD rats aged 8-12 weeks were randomly divided into 6 groups: control group (S group) and ischemic reperfusion group (group D) treated with IR after ischemia. Blood urea nitrogen (BUN) and creatinine (SCr), (SCr),) were measured. The successful IPO model was screened out. Then 6 male adult SD rats were divided into two groups: ischemia reperfusion group and IPO group. In the ischemia-reperfusion group, the right renal pedicle was ligated, the left renal pedicle 60min was clipped and the perfusion was restored. In the IPO group, the circulation was blocked for 10 s for 6 times and then the perfusion was fully opened after 10 s of renal ischemia 60min. After feeding for 24 hours, the left kidney was quickly removed and stored in liquid nitrogen. RNA extraction, probe preparation and chip hybridization were performed immediately after hybridization. Softfire Decisionsite8.0 software was used to analyze whether a gene was expressed and whether there were differences between the two groups. According to the function of gene encoding protein, the differentially expressed genes were preliminarily analyzed. Results there were different expression of 70 genes in renal tissues between the post-ischemic group and the ischemia-reperfusion group. There were 48 up-regulated genes and 22 down-regulated genes. These genes are mainly involved in cell metabolism and injury, apoptosis-related proteins, signal transduction and transcription, inflammatory related factors, oxidative stress related factors and so on. Conclusion IPO rat model of renal ischemia-reperfusion injury was successfully established. IPO may act on multiple gene targets to alleviate renal ischemia-reperfusion injury.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R692

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