【摘要】：根皮素是存在于蘋(píng)果、梨等水果及多種蔬菜汁液中的天然活性物質(zhì),具有抗腫瘤活性,能夠有效地誘導(dǎo)細(xì)胞凋亡、抑制細(xì)胞增殖.本研究初步探討了根皮素誘導(dǎo)前列腺癌LNCaP細(xì)胞的凋亡和相關(guān)分子機(jī)制.通過(guò)形態(tài)學(xué)觀察、噻唑藍(lán)(MTT)比色實(shí)驗(yàn)、CCK-8實(shí)驗(yàn)測(cè)定前列腺癌細(xì)胞活性;采用DAPI染色法測(cè)定細(xì)胞核凝縮破裂;運(yùn)用Annexin V-FITC/PI雙染流式細(xì)胞術(shù)測(cè)定前列腺癌細(xì)胞的凋亡率;再通過(guò)PI單染流式細(xì)胞術(shù)測(cè)定前列腺癌細(xì)胞的周期變化;以及采用Western blot檢測(cè)PI3K/AKT信號(hào)通路及其下游細(xì)胞凋亡相關(guān)蛋白和細(xì)胞周期相關(guān)蛋白的表達(dá).實(shí)驗(yàn)結(jié)果表明:根皮素誘導(dǎo)前列腺癌LNCaP細(xì)胞周期阻滯在G2/M期,并呈濃度依賴性降低細(xì)胞活性、增加細(xì)胞核凝縮破裂,顯著提高前列腺癌細(xì)胞的凋亡率.在分子機(jī)制上,根皮素呈現(xiàn)濃度依賴性下調(diào)PI3K/AKT的活性,從而影響細(xì)胞凋亡相關(guān)蛋白和細(xì)胞周期相關(guān)蛋白的表達(dá).因此,根皮素對(duì)前列腺癌細(xì)胞的生長(zhǎng)有顯著的抑制作用,對(duì)治療前列腺癌具有潛在的臨床應(yīng)用價(jià)值.
[Abstract]:Phyllostatin is a natural active substance in fruits and vegetables such as apple pear and so on. It has anti-tumor activity and can effectively induce cell apoptosis and inhibit cell proliferation. In this study, the apoptosis of prostate cancer LNCaP cells induced by phylloderin and its related molecular mechanisms were investigated. Morphological observation, (MTT) colorimetric assay, CCK-8 assay, DAPI staining, Annexin V-FITC/PI double staining flow cytometry were used to determine the apoptosis rate of prostate cancer cells. The cell cycle changes of prostate cancer cells were detected by PI single staining flow cytometry, and the expression of apoptosis-related proteins and cell cycle associated proteins in PI3K/AKT signaling pathway and its downstream were detected by Western blot. The results showed that LNCaP cell cycle arrest in G _ 2 / M phase was induced by phylloderin in a concentration-dependent manner, which decreased cell activity, increased nuclear condensation and increased apoptosis rate of prostate cancer cells. In molecular mechanism, hirsutin down-regulated the activity of PI3K/AKT in a dose-dependent manner, thus affecting the expression of apoptosis-related proteins and cell cycle related proteins. Therefore, the growth of prostate cancer cells was significantly inhibited by phylloderin, which had a potential clinical value in the treatment of prostate cancer.
【作者單位】： 南京師范大學(xué)生命科學(xué)學(xué)院江蘇省分子與醫(yī)學(xué)生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(81272850、81472415)
【分類號(hào)】：R737.25

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 冷向鋒;TRAIL與前列腺癌[J];國(guó)外醫(yī)學(xué).泌尿系統(tǒng)分冊(cè);2003年03期

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