【摘要】：目的高遷移率蛋白N2(HMGN2)對(duì)膀胱癌T24細(xì)胞生長(zhǎng)和裸鼠移植瘤的抑制作用及其機(jī)制的研究。方法誘導(dǎo)表達(dá)得到重組蛋白HMGN2;在體外,MTT及流式細(xì)胞術(shù)檢測(cè)HMGN2對(duì)T24細(xì)胞的生長(zhǎng)抑制作用;在體內(nèi),建立T24細(xì)胞裸鼠移植瘤模型,瘤體周邊分別注射PBS、HMGN2和順鉑(DDP),檢測(cè)各組腫瘤的體積及質(zhì)量并計(jì)算抑瘤率。Western blot檢測(cè)凋亡蛋白Bcl-2及Bax的表達(dá)。結(jié)果 MTT及流式結(jié)果表明,HMGN2抑制了T24細(xì)胞的生長(zhǎng)且使T24細(xì)胞阻滯于細(xì)胞周期的S期;裸鼠移植瘤實(shí)驗(yàn)中,HMGN2組和DDP組腫瘤體積及瘤重明顯小于PBS對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05),兩組的抑瘤率分別為25%和23%;Western結(jié)果顯示,與PBS組相比,HMGN2組和DDP組能明顯下調(diào)瘤組織內(nèi)Bcl-2的表達(dá)(P0.05),且誘導(dǎo)Bax的表達(dá)(P0.05)。結(jié)論 HMGN2能明顯抑制T24細(xì)胞的增殖和裸鼠移植瘤的生長(zhǎng),其作用機(jī)制可能與影響細(xì)胞周期以及誘導(dǎo)細(xì)胞凋亡有關(guān)。
[Abstract]:Objective to investigate the inhibitory effect and mechanism of high mobility protein N2 (HMGN2) on the growth of bladder cancer cell line T24 and transplanted tumor in nude mice. Methods Recombinant protein HMGN2; was induced to express in vitro, MTT and flow cytometry were used to detect the inhibitory effect of HMGN2 on the growth of T24 cells. The tumor volume and mass were measured by peripheral injection of PBS,HMGN2 and cisplatin (DDP), and the expression of apoptotic protein Bcl-2 and Bax were detected by. Western blot. Results MTT and flow cytometry showed that HMGN2 inhibited the growth of T24 cells and blocked T24 cells in S phase of cell cycle, the tumor volume and tumor weight in HMGN2 and DDP groups were significantly lower than those in PBS control group. The results showed that compared with PBS group, HMGN2 group and DDP group could significantly down-regulate the expression of Bcl-2 in tumor tissue (P0.05), and induce the expression of Bax (P0.05). Conclusion HMGN2 can significantly inhibit the proliferation of T24 cells and the growth of xenografts in nude mice, which may be related to the influence of cell cycle and the induction of apoptosis.
【作者單位】： 新疆醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院;昌吉職業(yè)技術(shù)學(xué)院;
【基金】：新疆維吾爾自治區(qū)高�？蒲杏�(jì)劃(編號(hào):XJE-DU2014I019) 國家自然科學(xué)基金(編號(hào):81660480)
【分類號(hào)】：R737.14

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