【摘要】：目的:使用N-甲基-N-亞硝基脲(N-methyl-N-nitrosourea,MNU)誘導SD大鼠膀胱腫瘤模型,觀察4-羥苯維胺酯(4-HPR)的對大鼠膀胱腫瘤的干預效果。方法:70只SD大鼠隨機分成對照組(A組)、造模組(B組)、造模處理組(C組、D組、E組),處理組分別予以4-HPR、阿霉素(ADM)、卡介苗(BCG)行膀胱內(nèi)灌注。給藥結束后1周處死大鼠,用免疫組化檢測大鼠膀胱腫瘤中COX-2的表達和微血管密度(MVD);用TUNEL法檢測凋亡。結果:標本病理結果提示4-HPR、BCG、ADM對MNU誘導腫瘤的發(fā)生有明確的干預效果(P0.05)。4-HPR能有效抑制COX-2的表達及微血管的生成并促使腫瘤細胞的凋亡(P0.05)。結論:4-HPR、BCG、ADM對MNU的致癌過程均有干預作用,4-HPR與ADM、BCG的抗癌作用無顯著性差異,且其副反應更少。
[Abstract]:Aim: to observe the intervention effect of 4 hydroxybenzimide (4-HPR) on bladder neoplasms in SD rats induced by N methyl N nitrosoureaurea (MNU). Methods 70 SD rats were randomly divided into control group (group A), model group (group B) and model group (group C and D group). The treated group was given 4-HPRand adriamycin (ADM), BCG (BCG) respectively. The rats were killed at 1 week after administration. The expression of COX-2 and microvessel density (MVD);) in bladder tumor were detected by immunohistochemistry and apoptosis was detected by TUNEL method. Results: the histopathological results showed that 4-HPR-BCG-ADM had a definite intervention effect on tumorigenesis induced by MNU (P0.05) .4-HPR could effectively inhibit the expression of COX-2 and the formation of microvessels and promote the apoptosis of tumor cells (P0.05). Conclusion there is no significant difference in the anticancer effect of BCGADM between ADM,BCG and 4-HPR in the carcinogenic process of MNU, and its side effects are less.
【作者單位】： 皖南醫(yī)學院弋磯山醫(yī)院泌尿外科;
【基金】：安徽省教育廳臨床醫(yī)學應用技術科研課題(2006KJ136C)
【分類號】：R737.14

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1 王亮,陳昭頡,王慶堂,曹文峰,簡q，

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