【摘要】：侵襲和轉(zhuǎn)移是前列腺癌患者的重要死因。目前人類對(duì)前列腺癌的認(rèn)識(shí)水平有限，常規(guī)的治療手段尚無法從根本上清除惡性腫瘤，因此明確前列腺癌侵襲、轉(zhuǎn)移發(fā)生的關(guān)鍵機(jī)制，尋找相應(yīng)的阻斷途徑，建立起一套行之有效的治療手段就顯得尤為重要。 前期研究發(fā)現(xiàn)只與前列腺癌高轉(zhuǎn)移細(xì)胞系PC3M特異性結(jié)合的短肽B04具有抑制PC3M細(xì)胞侵襲和轉(zhuǎn)移的能力，因此我們認(rèn)為其在PC3M細(xì)胞上的受體可能與前列腺癌的轉(zhuǎn)移有關(guān)。 為了獲取特異性短肽B04的受體，本研究采用western blot、考馬斯亮藍(lán)染色、銀染色等方法初步定位特異性短肽B04受體的表達(dá)，繼而采用親和層析和質(zhì)譜鑒定等方法鑒定受體蛋白的身份信息，，并通過免疫熒光細(xì)胞化學(xué)染色的方法對(duì)受體蛋白的亞細(xì)胞定位進(jìn)行分析。結(jié)果證實(shí)短肽B04的受體蛋白為PC3M細(xì)胞的膜蛋白，成功地鑒定出ATP5B為特異性短肽B04的受體蛋白之一；證明ATP5B表達(dá)于前列腺癌高轉(zhuǎn)移細(xì)胞系PC3M的線粒體和細(xì)胞膜上，發(fā)現(xiàn)ATP5B為前列腺癌轉(zhuǎn)移相關(guān)蛋白；ATP5B表達(dá)于細(xì)胞膜上可能是其發(fā)揮轉(zhuǎn)移相關(guān)功能的關(guān)鍵。
[Abstract]:Invasion and metastasis are important causes of death in patients with prostate cancer. At present, the level of human understanding of prostate cancer is limited, conventional treatment methods can not fundamentally remove malignant tumors, so the key mechanism of prostate cancer invasion and metastasis is identified, and the corresponding blocking pathway is found. It is particularly important to establish a set of effective treatment methods. Previous studies have shown that the short peptide B04, which specifically binds to high metastatic prostate cancer cell line PC3M, can inhibit the invasion and metastasis of PC3M cells. Therefore, we believe that its receptor on PC3M cells may be related to the metastasis of prostate cancer. In order to obtain the receptor of specific short peptide B04, western blot, Coomassie brilliant blue staining and silver staining were used to preliminarily localize the expression of specific short peptide B04 receptor. Then the identity information of the receptor protein was identified by affinity chromatography and mass spectrometry, and the subcellular localization of the receptor protein was analyzed by immunofluorescence cytochemical staining. The results showed that the receptor protein of short peptide B04 was the membrane protein of PC3M cells, and that ATP5B was one of the receptor proteins of short peptide B04, and that ATP5B was expressed in mitochondria and cell membrane of high metastatic prostate cancer cell line PC3M. It was found that the expression of ATP5B in the cell membrane of the metastasis associated protein of prostate cancer may be the key to its metastasis-related function.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.25

【共引文獻(xiàn)】

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