【摘要】：目的:本研究就circCD44-360(hsa_circ_0021727)與circCD44-594(hsa_circ_0021728)在膀胱癌及癌旁組織中的表達(dá)情況,及它們對CD44基因表達(dá)的影響和相關(guān)性分析進(jìn)行研究,旨在為膀胱癌的診斷和靶向治療提供新的方向。方法:收集膀胱癌及癌旁組織的臨床標(biāo)本,處理標(biāo)本后提取膀胱癌及癌旁組織的總RNA,反轉(zhuǎn)錄獲取目的RNA,進(jìn)行實時定量PCR,檢測circCD44-360與circCD44-594的表達(dá)差異,并分析其分別同親本基因CD44的信使RNA(messenger RNA,mRNA)的關(guān)系進(jìn)而找出其調(diào)節(jié)機制。結(jié)果:1 CircCD44-360與circCD44-594在膀胱癌組織的表達(dá)均上調(diào)。PCR結(jié)果顯示,與癌旁組織相比,circCD44-360與circCD44-594在膀胱癌組織中的表達(dá)水平顯著升高。說明circCD44-360與circCD44-594在膀胱癌組織中的表達(dá)水平均顯著上調(diào)。2 CD44mRNA在膀胱癌組織的表達(dá)上調(diào)。PCR結(jié)果顯示,與癌旁組織相比,CD44mRNA在膀胱癌組織中的表達(dá)水平顯著升高。說明CD44mRNA在膀胱癌組織中的表達(dá)水平均顯著上調(diào)。3 CircCD44-360與circCD44-594均同其親本基因CD44的表達(dá)呈線性正相關(guān)關(guān)系。利用PCR結(jié)果進(jìn)行統(tǒng)計學(xué)分析,circCD44-360與其親本基因CD44的Pearson's correlation線性相關(guān)關(guān)系,R=0.5702,P=0.0087,呈正相關(guān)。另一個環(huán)狀circCD44-594與其親本基因CD44的Pearson's correlation線性相關(guān)關(guān)系,R=0.4474,P=0.0420,呈正相關(guān)。結(jié)論:1 CircCD44-360在膀胱癌組織中表達(dá)上調(diào)。2 CircCD44-594在膀胱癌組織中表達(dá)上調(diào)。3在膀胱癌中,circCD44-360、circCD44-594和CD44的表達(dá)呈正相關(guān)關(guān)系,它們可能通過調(diào)節(jié)CD44基因的表達(dá)促進(jìn)膀胱癌的發(fā)生和發(fā)展。
[Abstract]:Objective: To study the expression of circCD44-360 (hsa_circ_0021727) and circCD44-594 (hsa_circ_0021728) in bladder cancer and para cancer tissues, and their influence on the expression of CD44 gene and the correlation analysis. The purpose of this study is to provide new directions for the diagnosis and target therapy of bladder cancer. Methods: to collect bladder cancer and para cancer tissue. After treating specimens, the total RNA of bladder cancer and para cancer tissue was extracted, RNA was obtained by reverse transcription, and the real-time quantitative PCR was used to detect the difference between circCD44-360 and circCD44-594, and the regulation mechanism of the RNA (messenger RNA, mRNA) with the parent gene CD44, respectively, was analyzed. The results were as follows: 1 CircCD44-360 and circC. The expression of D44-594 in bladder cancer tissues was up regulated by.PCR, and the expression level of circCD44-360 and circCD44-594 in bladder cancer tissue was significantly higher than that of para cancerous tissue. The expression level of circCD44-360 and circCD44-594 in bladder cancer tissues was significantly up-regulated and the expression of.2 CD44mRNA in bladder cancer tissue was up to.PCR results. The expression level of CD44mRNA in bladder cancer tissue was significantly higher than that of para cancerous tissue. It indicated that the expression level of CD44mRNA in bladder cancer tissues was significantly up-regulated and that the expression of.3 CircCD44-360 and circCD44-594 were linearly correlated with the expression of their parent gene CD44. Statistical analysis of PCR knot fruit, circCD44-360 and its parents The Pearson's correlation linear correlation of gene CD44, R=0.5702, P=0.0087, is positively correlated. The other circular circCD44-594 is correlated with the Pearson's correlation linear correlation of the parent gene CD44, R=0.4474, P=0.0420. Conclusion: 1 CircCD44-360 in bladder cancer tissues is up regulated in bladder cancer tissue. The expression of.3 in bladder cancer is positively correlated with the expression of circCD44-360, circCD44-594 and CD44. They may promote the development and development of bladder cancer by regulating the expression of CD44 gene.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.14

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