【摘要】：目的初步探討高遷移率族蛋白1(high mobility group protein B1,HMGB1)和腫瘤壞死因子α誘導(dǎo)蛋白3(tumor necrosis factorα-induced protein-3,TNFAIP3)在狼瘡性腎炎(lupus nephritis,LN)患者和人類腎小球系膜細胞(human mesangial cell,HMC)增殖中的作用及機制。方法收集Ⅳ型LN患者的腎穿樣本,采用免疫熒光和免疫組化技術(shù)檢測腎小球細胞中HMGB1、TNFAIP3及IκBα蛋白表達水平;運用人重組HMGB1為刺激物刺激HMC,采用Brd U參入技術(shù)檢測細胞增殖水平,并用Western blot技術(shù)檢測TNFAIP3、IκBα及p65表達水平。結(jié)果與對照組相比,LN患者腎小球細胞中HMGB1及TNFAIP3蛋白表達升高,IκBα表達水平降低;體外實驗證實,人重組HMGB1刺激HMC后,細胞增殖水平明顯升高,同時在HMGB1刺激30 min后,與對照組相比,TNFAIP3的蛋白表達水平明顯升高(P0.05),而IκBα蛋白表達水平降低(P0.05),隨后p65蛋白表達明顯升高(P0.05)。結(jié)論 HMGB1和TNFAIP3可能通過活化NF-κB信號通路促進LN系膜細胞的過度增殖。
[Abstract]:Objective to investigate the role and mechanism of high mobility group protein 1 (high mobility group protein B1 (HMGB1) and tumor necrosis factor- 偽 inducible protein 3 (tumor necrosis factor 偽 -induced protein-3 (TNFAIP3) in the proliferation of glomerular Mesangial cells in patients with lupus nephritis (lupus nephritis LN) and in human glomerular Mesangial cells. Methods Renal puncture samples from patients with type 鈪，

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