【摘要】：目的:不孕不育已成為一個全球性的問題,正受到越來越多的關注。在引起不孕不育的因素中,男女雙方各占50%。男性不育是一個復雜、多因素疾病,其中遺傳因素是重要病因之一。下丘腦-垂體-性腺軸中的黃體生成素以及抗氧化物通路中的酶[超氧化物歧化酶2(SOD2)、對氧磷酯酶1(PON1)、醌氧化物還原酶1(NQO1)]對精子的生成都起著不可替代的作用。黃體生成素與抗氧化物酶基因的多態(tài)性會影響黃體生成素以及抗氧化物酶的活性,影響其功能的正常發(fā)揮,進而造成生精障礙。雖然國內外對黃體生成素基因多態(tài)性以及抗氧化物通路中酶基因多態(tài)性與男性不育之間的相關性進行了研究,但是由于樣本量大小及種群選取范圍不同,導致研究結果也各不相同。為了更加清楚的認識黃體生成素基因多態(tài)性以及抗氧化物通路基因多態(tài)性與男性不育之間的關系。本研究分析了LHB(rs34349826和rs6521),SOD2 rs4880,PON1 rs662以及NQO1 rs1800566與男性不育發(fā)病風險的相關性。方法:利用病例-對照組的方法共收集829例樣本,包括405例不育男性(非梗阻性無精子癥145例、嚴重少精子癥173例以及輕度少精子癥87例)作為病例組,424例已正常生育的男性作為對照組,利用Mass ARRAY i PLEX GOLD分型檢測技術對LHB rs34349826、LHB rs6521、SOD2 rs4880、PON1 rs662以及NQO1rs1800566共4個基因5個位點進行分型,并利用SPSS20.0對分型結果進行l(wèi)ogistic回歸分析。結果:通過病例組與對照組比較,在年齡、雌二醇以及睪酮這3項水平上無統(tǒng)計學差異,黃體生成素、卵泡刺激素存在有顯著的差異(P0.05)。logistic回歸分析結果顯示LHB rs34349826以及LHB rs6521與男性不育之間無明顯相關性,而在單倍型分析中,A-G(LHB rs34349826-rs6521)單倍型可以降低少精子癥組男性不育的發(fā)病風險(P=0.004,OR=0.33,95%CI=0.15-0.72)。G-G單倍型基因對男性不育來說是一個保護因素(總病例組中:P=0.000,OR=0.52,95%CI=0.42-0.65,非梗阻性無精子癥組中:P=0.001,OR=0.54,95%CI=0.40-0.75,嚴重少精子癥組中:P=0.013,OR=0.70,95%CI=0.53-0.93,輕度少精子癥組中:P=0.000,OR=0.22,95%CI=0.13-0.37)。SOD2 rs4880、PON1 rs662、NQO1 rs1800566與男性不育之間無明顯相關性,并且基因-基因交互作用與男性不育之間也沒有相關性。結論:LHB rs34349826、rs6521與男性不育之間不存在相關性,但單倍型分析發(fā)現,G-G單倍型可降低男性不育的發(fā)病風險,對男性不育來說是一個保護因素。SOD2 rs4880、PON1 rs662、NQO1 rs1800566與男性不育之間無明顯相關性,并且基因-基因交互作用與男性不育之間也無明顯相關性。
[Abstract]:Objective: infertility has become a global problem and is receiving more and more attention. Among the factors causing infertility, 50 are male and female. Male infertility is a complex, multivariate disease, in which genetic factors are one of the important causes. Luteinizing hormone in hypothalamus-pituitary-gonad axis and enzymes in antioxidant pathway [superoxide dismutase 2 (SOD2), p-oxaphosphatase 1 (PON1), quinone oxide reductase 1 (NQO1)] play an irreplaceable role in spermatogenesis. The polymorphism of luteinizing hormone and antioxidant enzyme gene will affect the activity of luteinizing hormone and antioxidant enzyme, affect the function of luteinizing hormone and antioxidant enzyme, and cause spermatogenesis obstacle. Although the relationship between luteinizing hormone gene polymorphism and enzyme gene polymorphism in antioxidant pathway and male infertility has been studied at home and abroad, the sample size and population selection range are different. The results also varied. In order to understand the relationship between luteinizing hormone gene polymorphism, antioxidant pathway gene polymorphism and male infertility. The relationship between LHB (rs34349826 and rs6521) SOD2 rs4880 PON1 rs662 and NQO1 rs1800566 and the risk of male infertility was analyzed. Methods: a total of 829 samples were collected from a case-control group, including 405 infertile men (145 non-obstructive azoospermia). 173 cases of severe oligozoospermia and 87 cases of mild oligozoospermia) were used as control group. Four loci of LHB rs349826 LHB rs652rs4880 PON1 rs662 and NQO1rs1800566 were classified by Mass ARRAY i PLEX GOLD. The results of typing were analyzed by logistic regression with SPSS20.0. Results: there was no significant difference in age, estradiol and testosterone between the case group and the control group. There was significant difference in follicle stimulating hormone (P0.05). Logistic regression analysis showed that there was no significant correlation between LHB rs34349826 and LHB rs6521 and male infertility. In haplotype analysis, haplotype A G (LHB rs34349826-rs6521) can reduce the risk of male infertility in oligozoospermia group (P0. 004). G-G haplotype gene is a protective factor for male infertility. G-G haplotype gene is a protective factor for male infertility. In the heavy oligozoospermia group, there was no significant correlation between the rs1800566 of P0: P0. 013, ORT 0.70rs662NQO1 and male infertility, while in the mild oligozoospermia group, there was no significant correlation between the percentage of 0. 2295) and the male sterility, and there was no significant correlation between the ratio of P0. 0. 013 and 0. 37) .SOD2 rs4880 and PON1 rs662NQO1 rs1800566. And there is no correlation between gene-gene interaction and male infertility. Conclusion there is no correlation between male sterility and male sterility, but haplotype analysis shows that G-G haplotype can reduce the risk of male infertility, and it is a protective factor for male infertility. There is no significant correlation between G-G haplotype and male infertility. There was no significant correlation between gene-gene interaction and male infertility.
【學位授予單位】：江蘇大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R698.2

【參考文獻】

相關期刊論文 前2條

1 龔道元;李子萍;莊錫偉;張曉林;康梅;廖異平;;男性不育患者精漿對氧磷酶-1活性測定的臨床價值[J];南方醫(yī)科大學學報;2012年09期

2 商學軍,黃宇烽,徐建平,王詠梅;超氧化物歧化酶在男性不育癥檢測中的意義[J];男科學報;1998年03期

，

本文編號：2155762