【摘要】：目的:探討辛伐他汀對前列腺上皮細胞RWPE-1增殖及凋亡的影響。方法:設(shè)定不同濃度的辛伐他汀(0、10、20、40μmol/L)分別作用于體外培養(yǎng)的RWPE-1細胞,利用MTT法檢測細胞增殖情況,流式細胞術(shù)檢測細胞的凋亡情況。熒光定量RT-PCR檢測RWPE-1細胞的Bcl-2、Bax、Cx43 mRNA的表達,Western印跡檢測Bcl-2、Bax、Cx43蛋白的表達。結(jié)果:MTT法檢測辛伐他汀(10、20、40μmol/L)作用于RWPE-1細胞72 h后,對RWPE-1細胞的抑制率分別為(21.07±6.41)%、(34.87±9.65)%和(47.18±10.88)%,與對照組[(1.21±0.54)%]比較有顯著差異(P0.05),并且呈明顯的劑量依賴關(guān)系(P0.05);處理72 h后各組的凋亡指數(shù)分別為:10μmol/L組(0.066±0.016)%,20μmol/L組(0.126±0.023)%,40μmol/L組(0.192±0.025)%,與對照組[(0.015±0.005)%]相比差異顯著(P0.01),且呈劑量依賴關(guān)系(P0.05)。熒光定量PCR檢測顯示隨著辛伐他汀濃度升高Bcl-2基因表達逐漸下調(diào)(P0.05),Bax和Cx43基因表達逐漸上調(diào)(P0.05),并且呈劑量依賴關(guān)系(P0.05)。Western印跡檢測顯示RWPE-1細胞內(nèi)Bcl-2蛋白的表達隨辛伐他汀濃度的增高逐漸下調(diào)(P0.05),Bax蛋白逐漸上調(diào)(P0.05),Cx43蛋白逐漸上調(diào)(P0.01),并且皆呈劑量依賴關(guān)系(P0.05)。Cx43的表達與Bcl-2的表達呈負相關(guān),與Bax的表達呈正相關(guān)。結(jié)論:辛伐他汀可能通過影響細胞間隙連接通訊來抑制前列腺上皮細胞增殖并誘導其凋亡。
[Abstract]:Aim: to investigate the effect of simvastatin on RWPE-1 proliferation and apoptosis in prostatic epithelial cells. Methods: different concentrations of simvastatin (0 10 ~ 2040 渭 mol/L) were used to treat RWPE-1 cells in vitro. Cell proliferation was detected by MTT assay and apoptosis was detected by flow cytometry. The expression of Bcl-2AXCx43 mRNA in RWPE-1 cells was detected by fluorescence quantitative RT-PCR. Western blot was used to detect the expression of Bcl-2OBaxCx43 protein. Results Simvastatin (10 ~ 20 渭 mol/L) was applied to RWPE-1 cells for 72 h. The inhibition rates of RWPE-1 cells were (21.07 鹵6.41), (34.87 鹵9.65)% and (47.18 鹵10.88), respectively, which were significantly different from those of the control group [(1.21 鹵0.54)%] (P0.05), and the apoptotic index of the control group was (0.066 鹵0.016) 渭 mol/L group (0.066 鹵0.016), (0.126 鹵0.023) 渭 mol/L group (0.192 鹵0.025) and (0.015 鹵0.005)% compared with the control group [(0.015 鹵0.005)%]. The difference was significant (P0.01), and showed a dose-dependent relationship (P0.05). Fluorescence quantitative PCR analysis showed that the expression of Bcl-2 gene decreased gradually (P0.05) and the expression of Cx43 gene increased gradually with the increase of simvastatin concentration (P0.05), and showed a dose-dependent relationship (P0.05) .Western blotting showed that the expression of Bcl-2 protein in RWPE-1 cells was increased with simvastatin. The increase of Tin-level was down-regulated (P0.05). (P0.05) the protein of Cx43 was up-regulated (P0.01), and the expression of Cx43 was negatively correlated with the expression of Bcl-2 in a dose-dependent manner (P0.05). There was a positive correlation with the expression of Bax. Conclusion: simvastatin may inhibit the proliferation and induce apoptosis of prostatic epithelial cells by affecting gap junctional communication.
【作者單位】： 福建醫(yī)科大學附屬漳州市醫(yī)院泌尿外科;
【基金】：福建省自然科學基金(2012J05164)~~
【分類號】：R697.3
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本文編號：2141345