發(fā)布時(shí)間：2018-06-15 16:09

  本文選題：慢性前列腺炎 + 精子��； 參考：《南華大學(xué)》2014年碩士論文

【摘要】：目的：探討不同原因的慢性前列腺炎患者是否合并精子DNA的損傷，進(jìn)一步了解不同原因的慢性前列腺炎所引起的精子DNA損傷有無(wú)不同。 方法：通過(guò)對(duì)160例納入患者按前列腺液常規(guī)的化驗(yàn)結(jié)果進(jìn)行慢性前列腺炎篩選和精液常規(guī)分析，按其前列腺液化驗(yàn)結(jié)果將其分為慢性前列腺炎合并細(xì)菌感染組（A組40例）、慢性前列腺炎合并支原體感染組（B組40例）、慢性前列腺炎合并其他原因組（C組40例）以及健康對(duì)照組（D組40例）。然后采用精子染色質(zhì)擴(kuò)散試驗(yàn)和彗星實(shí)驗(yàn)2種方法分別檢測(cè)患者精子DNA的完整性，其中精子染色質(zhì)擴(kuò)散實(shí)驗(yàn)以精子DNA斷裂指數(shù)表示其損傷程度，，即精子DNA損傷定性實(shí)驗(yàn)；彗星實(shí)驗(yàn)以彗星尾部DNA含量的百分比表示其損傷程度，對(duì)精子染色質(zhì)擴(kuò)散實(shí)驗(yàn)的結(jié)果進(jìn)行進(jìn)一步驗(yàn)證，即精子DNA損傷定量實(shí)驗(yàn)。最后通過(guò)統(tǒng)計(jì)分析得出A、B、C、D四組是否合并精子DNA損傷及組組之間精子DNA損傷的程度，進(jìn)一步了解不同原因慢性前列腺炎所引起的精子DNA損傷有無(wú)不同。 結(jié)果： 對(duì)160例病例行精子染色質(zhì)擴(kuò)散實(shí)驗(yàn)得出：細(xì)菌感染組、支原體感染組、其他原因組SCD損傷率高于健康對(duì)照組SCD損傷率，差異有統(tǒng)計(jì)學(xué)意義（p0.005）。細(xì)菌感染組SCD損傷率高于支原體感染組、其他原因組SCD損傷率，差異有統(tǒng)計(jì)學(xué)意義（p0.005）。支原體感染組SCD損傷率與其他原因組SCD損傷率比較無(wú)統(tǒng)計(jì)學(xué)意義（p0.05）。彗星實(shí)驗(yàn)得出：細(xì)菌感染組彗星尾長(zhǎng)百分比為47.44±25.80，支原體感染組彗星尾長(zhǎng)百分比為22.96±21.40，其他原因組彗星尾長(zhǎng)百分比為22.02±21.92，健康對(duì)照組彗星尾長(zhǎng)百分比為6.51±9.85。細(xì)菌感染組、支原體感染組、其他原因組彗星尾長(zhǎng)百分比高于健康對(duì)照組彗星尾長(zhǎng)百分比，差異有統(tǒng)計(jì)學(xué)意義（p0.005）。細(xì)菌感染組彗星尾長(zhǎng)百分比高于支原體感染組和其他原因組彗星尾長(zhǎng)百分比，差異有統(tǒng)計(jì)學(xué)意義（p0.005），支原體感染組彗星尾長(zhǎng)百分比與其他原因組彗星尾長(zhǎng)百分比比較無(wú)統(tǒng)計(jì)學(xué)意義（p0.05）�？烧J(rèn)為：各實(shí)驗(yàn)組與對(duì)照組之間精子DNA損傷有差別，各實(shí)驗(yàn)組精子DNA損傷率高于健康對(duì)照組。A組的精子DNA損傷程度高于B組、C組的精子DNA損傷。但還不能認(rèn)為B組與C組的精子DNA損傷率有差別。 結(jié)論： 1.慢性前列腺炎可以引起不同程度的精子DNA損傷。 2.引起慢性前列腺炎的原因不同，精子DNA損傷程度有所不同，其中以細(xì)菌感染所致精子DNA損傷程度最嚴(yán)重，支原體感染和其他原因所致的慢性前列腺炎精子DNA也有不同程度的損傷。 3.精子DNA損傷的檢測(cè)可能成為臨床上研究男性不育的一個(gè)重要的指標(biāo)。
[Abstract]:Objective: to investigate whether the patients with chronic prostatitis have different sperm DNA damage, and to find out if there are differences in sperm DNA damage caused by chronic prostatitis. Methods: chronic prostatitis screening and semen routine analysis were carried out in 160 patients with prostatic fluid. According to the test results of prostatic fluid, it was divided into four groups: chronic prostatitis with bacterial infection group (n = 40), chronic prostatitis with mycoplasma infection group (n = 40), chronic prostatitis with other causes group (n = 40) and chronic prostatitis with mycoplasma infection group (n = 40). And healthy control group (n = 40). Then sperm chromatin diffusion test and comet assay were used to detect the integrity of sperm DNA respectively. The sperm chromatin diffusion test showed the degree of damage by sperm DNA break index, that is, the qualitative test of sperm DNA damage. The percentage of DNA content in comet tail indicated the degree of damage. The result of sperm chromatin diffusion test was further verified, that is, the quantitative assay of sperm DNA damage. Finally, the statistical analysis was conducted to find out whether the four groups were combined with sperm DNA damage and the degree of sperm DNA damage between groups, and to find out whether there were differences in sperm DNA damage caused by chronic prostatitis. Results: the sperm chromatin diffusion test in 160 cases showed that the SCD damage rate in bacterial infection group, mycoplasma infection group and other cause group was higher than that in healthy control group, and the difference was statistically significant (P 0.005). The SCD damage rate of bacterial infection group was higher than that of mycoplasma infection group, and the difference of SCD damage rate in other cause group was statistically significant (P 0.005). The SCD damage rate of mycoplasma infection group was not significantly higher than that of other causes group (P 0.05). Comet assay showed that the percentage of comet tail length was 47.44 鹵25.80 in bacterial infection group, 22.96 鹵21.40 in mycoplasma infection group, 22.02 鹵21.92 in other cause groups and 6.51 鹵9.85 in healthy control group. The percentage of comet tail length in bacterial infection group, mycoplasma infection group and other cause group was higher than that in healthy control group (P 0.005). The percentage of comet tail length in bacterial infection group was higher than that in mycoplasma infection group and other cause group. The percentage of comet tail length in mycoplasma infected group was not significantly higher than that in other cause groups (p 0.05). It can be concluded that the sperm DNA damage rate of each experimental group is higher than that of healthy control group. The degree of sperm DNA damage in group A is higher than that in group B and C. But it can not be concluded that there is difference between group B and group C in sperm DNA damage rate. Conclusion: 1. Chronic prostatitis can cause different degrees of sperm DNA damage. 2. The causes of chronic prostatitis are different, and the degree of sperm DNA damage is different, among which the degree of sperm DNA damage caused by bacterial infection is the most serious. Mycoplasma infection and other causes of chronic prostatitis caused by sperm DNA damage to varying degrees. The detection of sperm DNA damage may be an important marker for the clinical study of male infertility.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R697.33

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