本文選題：不育癥 + DNA損傷��； 參考：《安徽醫(yī)科大學》2014年碩士論文

【摘要】：目的：運用精子染色質擴散實驗(SCD)分析精子DNA損傷率和熒光原位雜交實驗(FISH)分析精子染色體（18、X、Y）非整倍體率。探討并分析男性不育患者精子DNA完整性和染色體畸變的發(fā)生率及臨床意義。 方法：精液標本來自正常已育的男性及不育男性患者，依據WHO標準行精液分析，根據精液分析結果及不育史分為對照組、少精癥組、弱精癥組、少弱精癥組。精子染色質擴散實驗（SCD）分析精子DNA損傷率和熒光原位雜交實驗（FISH）分析精子染色體（18、X、Y）非整倍體率。 結果：經統(tǒng)計學分析表明少精癥組、弱精癥組和少弱精癥組患者精子DNA損傷率對照組相比差異有顯著統(tǒng)計學意義（P＜0.01）；不育組中的精子染色體（18、X、Y）非整倍體率與對照組比較差有統(tǒng)計學意義（P＜0.05）；精子DNA損傷率與染色體非整倍體率之間呈顯著正相關（r=0.453，P=0.008，n=33）。 結論：不育患者精子DNA損傷率和染色體非整倍體率較正常對照組明顯升高且具有顯著的正相關性。在生殖細胞減數分裂過程中精子DNA損傷可能是導致非整倍體精子形成的重要原因之一。
[Abstract]:Objective: to analyze the rate of DNA damage in sperm by sperm chromatin diffusion assay (SCD) and the rate of aneuploidy by fluorescence in situ hybridization (fish). To investigate and analyze the incidence and clinical significance of sperm DNA integrity and chromosome aberration in infertile men. Methods: semen samples were collected from normal fertile men and infertile men. Semen analysis was performed according to WHO standard. According to the results of semen analysis and history of infertility, semen samples were divided into control group, oligozoospermia group and oligozoospermia group. Sperm chromatin diffusion assay (SCD) and fluorescence in situ hybridization (fish) were used to analyze the rate of DNA damage and aneuploidy. Results: the difference of sperm DNA damage rate between oligozoospermia group, asthenospermia group and oligozoospermia group was significant (P < 0.01). The rate of aneuploidy in infertile group was significantly lower than that in control group (P < 0.05), and there was a significant positive correlation between DNA damage rate and chromosome aneuploidy rate (P < 0.05), and there was a significant positive correlation between sperm DNA damage rate and chromosome aneuploidy rate (P < 0.05), and there was a significant positive correlation between sperm DNA damage rate and chromosome aneuploidy rate (P < 0.05). Conclusion: sperm DNA damage rate and chromosome aneuploid rate in infertile patients were significantly higher than those in normal controls. DNA damage of sperm during meiosis may be one of the important reasons for aneuploidy spermatogenesis.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R698.2

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本文編號：2020687